Investigating the effect of changing substrate concentration on the activity of the enzyme catalase The aim of this experiment is to examine how the concentration of a substrate (hydrogen peroxide) affects the rate of reaction of an enzyme catalyse (found in liver cells) Research Question: how does changing the concentration of the substrate affect the rate of reaction of the enzyme catalyse? Hypothesis: As the concentration of the substrate increases‚ so does the rate of reaction until the reaction
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Enzyme Activity Lab Hypothesis: 1] Are enzymes reusable? Yes‚ enzymes are reusable. 2] Which of the following do you think contain catalase? [Manganese dioxide‚ chicken meat‚ apple‚ carrot‚ potato‚ or chicken liver] I think that the manganese dioxide‚ apple‚ and carrot contain the catalase. 3] Would grinding the materials affect enzyme activity? I think that grinding the materials will increase enzyme activity. 4] Would temperature affect enzyme activity? I think that
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I am choosing to study how the PSI (pounds per square inch) of a football is affected by the temperature. I chose to study this because I like football and I always pondered this question. I believe that this is a worthwhile question to study because these studies can help improve the game as the weather gets colder. As air cools‚ it expands‚ so when a football gets colder‚ the air inside it expands. This expansion causes the air pressure to decrease. The air pressure decreases because the molecules
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The effect on rate of an enzyme catalysed reaction by different objectives which include effect of substrate‚ temperature‚ ph and effect of a competitive inhibitor phosphate ions. This is determined by the reaction of hydrolysis by p-nitrophenylphosphate (PNP) as a substrate by the enzyme phosphatase. Abstract The hydrolysis of p-nitrophenyl phosphate has been studied in human red blood cells. To see if hydrolysis was related to the functioning of the sodium pump. Acid phosphatase catalysis’s
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Study of the first element – Hydrogen. Answer the following questions:- 1. Give a reason why hydrogen can be placed in group 1[IA] and group 17[vIIA] of the periodic table. 2. What similarities does it show with group 1[IA] and group 17[VIIA]. With special reference to valency electrons and ion formaton and examples. 3. How does hydrogen occur in the free and combined state? 4. Which metals react with cold‚steam and boiling water to form their respected oxides and hydroxides ? Give examples and
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Investigate the effect of temperature on amylase activity Introduction Amylase is an enzyme that catalyses the breakdown of starch into sugars. Amylases are found in almost all plants‚ animals and microorganisms. Large amounts of amylase occur in germinating cereals‚ and in the pancreas and saliva of higher animals. Aim The aim of this experiment is to find out the rate of reaction between amylase and starch in a range of different reaction temperatures. Hypothesis As the reaction
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Lab Report (Effect of concentration on enzyme activity) Biology Noor Alawadhi 11- KC Introduction: An Enzyme is a protein‚ which is capable of starting a chemical reaction‚ which involves the formation or breakage of chemical bonds. A substrate is the surface or material on or from which an organism lives‚ grows‚ or obtains its nourishment. In this case it is hydrogen peroxide. This lab report will be explaining the experiment held to understand the effects of the changes in the amount of
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PROCEDURE: Part A (Effect of temperature on growth) 1) 15 tubes of glucose broth are provided and one set of 3 tubes are inoculated with each of the following cultures; Escherichia coli‚ Pseudomonas fluorescens‚ Micrococcus luteus and Saccharomyces cerevisiae. The last served as control. 2) One of the three tube of each culture is incubated at each of the following temperature: * 4°C * 37°C * 55°C 3) All the tubes are incubated within 5 minutes after inoculating. The turbidity
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15 April 2013 9:01 AM - DNA = Deoxyribonucleic acid DNA is a double-helix: it has two strands that twist around each other Each strand is made of single units call nucleotides It has a sugar-phosphate backbone Bases join the two strands by hydrogen bonds ○ These bases are cytosine‚ guanine‚ adenine and thymine. - Complementary base pairing is a key idea in genetics: C pairs with G‚ and T pairs with A. - Each strand of DNA can be millions of base pairs in length and is coiled up to make chromosomes
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– The effect of substrate concentration on the rate of enzyme activity of Catalase Aim To investigate the effect of substrate concentration (manipulated by increasing concentration of hydrogen peroxide) on the rate of enzyme activity of catalase‚ produced by liver cells‚ on the decomposition of hydrogen peroxide. Introduction Enzymes are biological catalysts that increase the rates of reactions. In an enzyme-catalyzed reaction‚ the substrate binds to the active site and forms enzyme-substrate
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