HYDROGEN BONDING: Hydrogen bonding is a bonding type consisting of dipole and dispersion forces. A hydrogen bond is the attractive force between a hydrogen atom attached to a molecule and an atom of a different molecule. According to the Pauling scale of electro-negativities of the elements‚ it can be viewed that the three most electronegative elements in the periodical table are nitrogen‚ oxygen and fluorine. These are also called heteroatoms. The heteroatoms have a partial negative charge while
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The Effect of pH on the Rate of Enzyme Catalysis of Catalase Objectives: The objective of this lab was to develop a protocol to investigate the effect of an environmental variable on the catalytic function of an enzyme. More specifically‚ the objective was to perform an experiment in order to test the effect of pH on the function of the enzyme catalase. Introduction: Enzymes are proteins that act as catalysts for reactions. This simply means that enzymes lower the activation energy required
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MEASURING pH Date of Lab: 02/09/13 Date Report submitted: 02/26/13 Purpose of the experiment By doing this experiment‚ we were trying to learn how to measure a pH with a pH meter to determine whether a solution is weakly‚ moderately or strongly acidic or basic; but also to compare the different data founded to see how the addition of certain liquid to a solution may affect the pH of the initial solution. Materials We used: * Tap water‚ distilled water‚ Soda and milk as the main
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Effect of changes in substrate concentration on the reaction rate of an enzyme IB biology Internal Assessment 3/23/12 Research Question: Effect of changes in substrate concentration amount on the reaction rate of an enzyme Introduction: In this experiment‚ the substrate is hydrogen peroxide. The purpose of this investigation is to find out the relationship between the substrate concentration and the rate of reaction. Substrates are molecules that are acted upon by enzymes. For instance
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Stallings Period 2 January 26‚ 2013 Enzyme Catalysis Lab Report Background: Enzymes are catalyst‚ which affect the rate of a chemical reaction. One consequence includes the cell to carry out complex chemical activities at relatively low temperatures. In these reactions the substrate binds reversibly to the active site. The cause of this is a decrease in the energy needed to activate the reaction of the substrate molecule to from products. Every enzyme is particular for a reaction for the reason
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____________________ Immobilizing an enzyme provides various analytical benefits‚ and can be done in a myriad of ways‚ with the most common being entrapment. For this study peroxidase (from horseradish)‚ an enzyme that catalyzes the cleavage of hydrogen peroxide into water‚ was entrapped within a polyacrylamide gel matrix. The gel matrix was formed by the addition of methylene bis-acrylamide (a cross linking agent) to acrylamide. The immobilized enzyme was then tested via spectrophotometric assay
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Title: Catalase enzyme detection Objective: To understand the function of catalase in cells that produce the enzyme‚ interpret the results of a catalase test and know their value in differentiating bacteria. Materials: 1 clean microscopic slide‚ 3% H2O2 solution‚ swabs. Micrococcus luteus‚ Enterococcus faecalis‚ patient G Procedure: 1) Scrape some cells off from each bateria to the slant and place them on glass slide. 2) Place one or two drops of H2O2. Watch for bubbling as an indication of
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Title: The Effect of Varying Amounts of Substrate and Enzyme on a Reaction Rate Abstract In living organisms‚ certain reactions must take place rapidly to assist life. This occurs because of enzymes‚ because all reactions would take place too slowly to sustain life (Jacklet‚ 237). Enzymes are large protein molecules that catalyze specific chemical reactions without being used up in the process. Each enzyme has a region on its surface‚ called the active site‚ which recognizes a specific
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Title: Amount of Oxygen‚ Mung Beans (Vigna Radiata) Consume during Cellular Respiration Introduction: Cellular respiration is a catabolic reaction that refers to the process of converting chemical energy of organic molecules into a simplify form so it can be used immediately by organism. Glucose may be oxidized completely if sufficient oxygen is available‚ by the following equation: C6H12O6 + 36 ADP + 36Pi + 6O2(g) 6 H2O + 6 CO2(g) + 38 ATP + heat All organisms‚ including plants and animals
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(3) Answer the questions related to each chemical formula. O2 CO2 5 H2 What element does the O represent? ___________________ How many atoms of each element are in the formula shown? C = ______ O = ______ How many atoms of Hydrogen are in this formula as shown? ______ 2C2H6 2Na2SO4 How many atoms each element are in the formula shown? C = ______ H = ______ How many atoms each element are in the formula shown? Na = ______ S = ______ O = ______ Part B: Label
Free Oxygen Nitrogen Methane