EFFECT OF TEMPERATURE‚PH AND SUBSTRATE CONCENTRATION ON ENZYME ACTIVITY | Aim To investigate the effect of Temperature‚Ph and substrate concentration on the rate of enzyme activity. Hypothesis
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Decomposition of Hydrogen Peroxide by Potassium Iodide Brief Description: Two solutions are mixed resulting in an eruption of foam resembling a huge stream of toothpaste. This is the classic “Elephant Toothpaste” reaction. Purpose/Goal: Procedure presents an example of a catalyzed reaction. Explanation of Experiment: The rapid evolution of oxygen gas is produced by the following reaction: 2 H2O2 (aq) = 2 H2O (l) + O2 (g) + heat The decomposition of hydrogen peroxide in the presence
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Title: “The Effect of Substrate Concentration‚ Enzyme Concentration‚ pH and Temperature on Enzyme Activity” Abstract: In the following experiments we will measure precise amounts of potato extract as well as Phenylthiourea‚ combined with or without deionized water and in some instances change the temperature and observe and record the reaction. We will also investigate the different levels of prepared pH on varying samples of the potato extract and the Phenylthiourea and record the results.
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Kinetics of the Decomposition of Hydrogen Peroxide Lab Introduction: In this week’s lab experiment‚ the rate of decomposition of hydrogen peroxide forming oxygen gas will be observed and studied. Since the rate of a chemical reaction is dependent on two things; the concentrations of the reactants and the temperature at which the process is performed‚ the rate can be measured at which a reactant disappears or at which a product appears. When measuring the rate‚ the rate law will be applied. The
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substrate (H2O2 (hydrogen peroxide)) have an effect on the activity of the enzyme (catalase)? Theory The higher the substrate concentration the more quickly product is produced (rate of reaction increases) until enzyme saturation is reached at which time more substrate has no further effect. Enzymes such as Catalase are protein molecules which are found in living cells. They are used to speed up specific reactions in the cells. They are all very specific as each enzyme just performs one
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Introduction: The purpose of this experiment is to measure the effects of changes in temperatures and pH on enzyme activity in skeletal muscle‚ particularly the activity of lactate dehydrogenase (LDH). LDH is a glycolytic enzyme which converts pyruvate to lactate in the following equation: LDH Pyruvate+ NADH ------------ Lactate + NAD The reaction above can move in both directions‚
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For many years Hydrogen peroxide was widely used for disinfecting‚ sterilization and its antiseptic properties‚ it is cheap and easily available OTC at any drug store or grocery store. “This readily available oxidant is rapidly converted to the highly reactive hydroxyl radical that damages an array of cellular components” (Atiyeh‚ Dibo‚ and Hayek‚ 2009). “The Medicines and Healthcare Products Regulatory Agency (MHRA‚ 2014). Have issued a warning regarding its use in surgery and in deep and large
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inside of the amylose coil.The amount of blue complex that starch gives with iodine can be measured by using a spectrophotometer. α-amylases are found in saliva‚ pancreatic juice‚ human breast milk‚ serum and certain tissues such as the liver. This enzyme catalyzes the hydrolysis of α (1-4) linkages in starch by breaking it down to maltose and some glucose. As the starch is broken down‚ the coiled structure of α-amylase is unfolded. Therefore‚ iodine will no longer be able to form the blue complex
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Enzymes are biological catalysts and each individual enzyme can only catalyse to one type of reaction – due to its specific shape. Each individual enzyme has its own specific shape which is determined by the amino acid sequence that it is made up of – each enzyme’s active site matches to its unique substrate molecule. For the sake of our experiment – enzymes catalyse reactions because they become an active site for reactions to take place. This lowers the energy that is needed for the reaction but
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The effect of enzyme concentration‚ substrate concentration‚ pH‚ and temperature on the enzyme catalase. Introduction: Enzymes are biological catalysts; proteins and RNA. They are required for most biological reactions and they are highly specific. Each enzyme has an active site. The active site is the spot on the enzyme where a substrate fits in. Substrates binds with enzymes through the active site. Enzymes‚ being highly specific‚ only fit with one certain substrate. Enzymes and substrates
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