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    Ap Bio Enzyme Lab

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    Introduction Enzymes are catalytic proteins. The purpose of a catalyst is to speed up metabolic reactions by lowering the free energy of activation or activation energy. Activation energy is known as the amount of energy needed to push the reactants over an energy barrier‚ so that the downhill part of the reaction can begin (Campbell 151). In an enzyme catalyzed reaction‚ the enzyme binds to its substrate‚ which is the reactant an enzyme acts on. In the reactions‚ the enzymes are very specific

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    Part one 1. a) When hydrogen peroxide was added to manganese‚ tiny bubbles formed in the solution- intensity of (1). b)The solution did not change in temperature. c) The bubbles stopped forming after about 18 minutes. 2. After we added 2 more mL of hydrogen peroxide‚ the reaction occurred again. Again‚ small bubbles formed; intensity of (1). The solution did not change in temperature. The reaction lasted about 15 minutes. 3. After adding more manganese dioxide‚ the reaction occurred again. This

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    Enzyme Catalysis Lab

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    Purpose: The purpose of this lab was to observe and understand the effects of changes in temperature‚ pH‚ enzyme concentration‚ and substrate concentration on the reaction rate of an enzyme-catalyzed reaction. Another purpose of the lab was to explain how environmental factors affect the rate of enzyme-catalyzed reactions. Hypothesis: I believe that if there is an increase in enzyme concentration‚ an increase in temperature‚ or an increase in pH‚ then the intensity of the reaction will increase

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    Protein and Hydrogen Bonds

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    at pH = 7 would be:  A. +2 B. +1 C. 0 D. -1 E. -2   5. The sequence of monomers in any polymer is this type of structure:  A. primary structure B. secondary structure C. tertiary structure D. quaternary structure E. All of these   6. Hydrogen bonds are most important in this type of structure in proteins:  A. primary structure B. secondary structure C. tertiary structure D. quaternary structure E. All of these   7. The overall folding of a single protein subunit is called:  A

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    The Effect of catalase reaction on temperature Table of Contents: Bibliography ………………………………………………………………………………………………….. Page 1 Introduction……………………………………………………………………………………………………Page 2-3 Methods/ Procedures……………………………………………………………………………………..Page 4-5 Results ………………………………………………………………………………………………………….. Page 6 Analysis of Data ……………………………………………………………………………………………. Page 6 Graph …………………………………………………………………………………………………………... Page 7 Conclusion……………………………………………………………………………………………………

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    Effect of Osmotic Stress and Temperature on Microbial Growth BIO 3400-002L – Microbiology Lab 1 Effect of Osmotic Stress and Temperature on Microbial Growth Luiz Felipe Isidoro ABSTRACT Evolution allowed primitive forms of life to develop proteins and enzymes that made it possible for life to evolve under environments with hostile conditions‚ such as high salt and heat. More specifically‚ some bacteria selected genes that code for peptides with stronger intermolecular forces‚ coping

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    Investigating the Effect of Concentration on the Rate of Enzyme Catalysed Reaction  To investigate the effect of concentration on the rate of enzyme  catalysed reaction I must know all the factors that affect it in order  to investigate in this.  Here are all the factors:  Temperature- Enzyme like it warm but not too hot. Enzymes are  biological catalysts‚ which speed up chemical reactions. They work  most efficiently at the optimum point (37°C). If they are below 37°C  they will work

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    Biochemistry Lab - Enzymes

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    Milk. Aims: 1. To determine the effect of ascorbic acid on Polyphenol Oxidase (Phenolase). 2. To determine the level of specificity of Phenolase using the following substrates: Caffeic Acid‚ Catecol‚ Guaicol‚ Pyragallol and Tyrosine. 3. To determine the effect of ascorbic acid on Peroxidase. 4. To determine the level of specificity of Peroxidase using the following substrates: Caffeic Acid‚ Catecol‚ Guaicol‚ Pyragallol and Tyrosine. 5. To calculate the effect of the substrate acetaldehyde concentration

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    Enzyme Activity

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    My lab group studied the effect of PH on reaction rate/ enzyme activity measured by foam height. PH is the measure of the concentration of hydrogen ions in a solution. The higher the hydrogen ion concentration‚ the lower the pH. Every enzyme has an optimal PH‚ meaning they have a very small window in which they are most active. Our enzyme (potato smoothie) had an optimal PH of 7.0-7.5. We know this because we measured the enzyme’s reaction rate by measuring foam height. The largest foam height we

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    8 1 EXPERIMENTAL DATA Table 1: Coordinate of Pressure Tapping Tapping No. 1 2 3 4 5 6 7 8 9 10 11 Note: Table 2: Pressure Readings Manometer inclination: Pressure Readings Pitot Pressure Static Pressure Atmospheric Pressure Atmospheric Temperature Stall angle: At the end of the experiment 474 mm 497 mm 500 mm 29°C (mm) 0.0 2.5 5.0 10 20 30 40 50 60 70 80 (mm) 0.000 3.268 4.443 5.853 7.172 7.502 7.254 6.617 5.704 4.580 3.279 0 0.025 0.049 0.098 0.197 0.295

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