Biology investigation: Liver and hydrogen peroxide By Cuong Tran Abstract: The purpose of this investigation was to determine the optimum temperature for enzyme activity. The stated hypothesis was that 37oC would be the optimum temperature for liver enzymes to break down hydrogen peroxide into water and oxygen. The dependent variable was to measure the amount of foam being produced‚ while testing 4 different temperatures with 3 repetitions each. Out of the 12 trials only 2 outliers were found
Premium Oxygen Hydrogen peroxide Enzyme
What effect does temperature have on the oxygen production of enzymes in an animal’s liver? A temperature which is too high would be able to produce little to no oxygen because of the extreme temperature. Just like a temperature too high‚ a temperature which is too low would also produce little to no oxygen for the same reason. Animals that have livers are considered warm-blooded‚ with very warm temperatures; therefore it would make sense for a warm temperature to produce the most oxygen; but one
Premium Gas Measurement Enzyme
Aim: To find the effect of temperature on enzymes‚ using a potato as a catalyst. The source of catalase is in the potato cells. 2H2O2 → O2 + H2O Planning: Introduction: An Enzyme is any one of many specialised organic substances‚ composed of polymers of amino acids‚ that act as catalysts to regulate the speed of the many chemical reactions involved in the metabolism of living organisms Enzymes are classified into several broad categories‚ such as hydrolytic‚ oxidising‚ and reducing‚ depending
Free Chemical reaction Enzyme Catalase
Analyzing a Hydrogen Peroxide Solution Purpose: The technological purpose of this investigation is to test and evaluate the percent concentration of consumer solution of hydrogen peroxide. Problem: What is the percent concentration of hydrogen peroxide in a consumer product? Design: An acidic solution of primary standard‚ iron (II) ammonium sulfate-water (1/6)‚ is prepared and the potassium permanganate standard. A 25.0 mL sample of a consumer solution of hydrogen peroxide is diluted to 1
Premium Hydrogen peroxide Titration Potassium permanganate
investigation to compare the reaction rates between potato and hydrogen peroxide against liver and hydrogen peroxide through loss in mass. Background information: Catalase is an enzyme that is found in all cells. This means that it is an intracellular enzyme. And enzyme is a biological catalyst. A catalyst is some thing that speeds up a reaction without being changed itself. Because of this enzymes and catalysts can be used again and again. Enzymes are protein chains that have a primary‚ secondary and tertiary
Premium Hydrogen peroxide Protein Potato
Effect of Temperature ( C ͦ) on Enzyme Catalase Activity in potato Aim: To investigate the Effect of temperature (10‚ 37‚ 60) Celsius (C ͦ) on enzyme catalase activity in potato using 2% of hydrogen peroxide (H202) as the substrate measuring the height (cm) of oxygen gas (bubbles) and calculating the volume of oxygen bubbles produced (cm3) Introduction: Enzymes are biological catalysts that speed up metabolic reactions without being affected. They lower the activation energy needed to start
Premium Catalase Enzyme Gas
To investigate the effect of temperature on an enzyme controlled reaction Introduction and planning For the investigation of enzymes‚ I am going to conduct an experiment to see how temperature can affect the rate of reaction of enzymes by testing it with starch. The enzyme that we are going to use is called amylase. We are going to test this enzyme with starch. By mixing amylase and starch solutions together under different temperature conditions‚ we can record the rate of reaction by taking a
Premium Enzyme
An investigation into the effects of temperature on enzyme action An enzyme is a biological catalyst that speeds up the rate of reaction in certain biological functions. They play a vital role in many aspects of human physiology and are necessary for the functioning of a number of systems‚ for example in the digestive system to help to break down food. All enzymes have a unique active site that can fit on to a particular molecular arrangement on a target substrate; a substance e.g. carbohydrate
Premium Temperature Chemical reaction Chemistry
when combined with hydrogen peroxide? Aim: To see find out how the amount of catalase correlates with the amount of gas created when in combination with hydrogen peroxide. Materials: 15g of liver 25 ml of hydrogen peroxide 1 scale 1 bucket 1 100ml graduated cylinder 1 250ml flask with bung 1 50cm tube (able to fix onto top of flask) 1 stopwatch 1 100ml beaker 1 funnel 1 pipette 1 microscopy kit Variables: Independent: This is the amount of liver added to the hydrogen peroxide. The initial weight
Premium Oxygen Hydrogen peroxide Enzyme
Objective: To investigates the enzymatic effect of various materials in the hydrogen peroxide solution. Apparatus & Equipment: Beaker Test tubes Either: water bath (95oC) or Bunsen burner Materials: Fresh Liver Potato cubes Manganese dioxide Hydrogen peroxide** Wood splints **Caution: Hydrogen peroxide is formed continuously as a by-product of chemical reactions in living cells; it is a very toxic (poisonous) substance. [Note: using boiling tubes may provide
Premium Oxygen Water Chlorine