Enzymes INTRODUCTION Enzymes are protein cells and they are found in the gut. They help to break down the food you eat. Enzymes are produced by living cells and they speed up reactions because they are biological catalysts. If we did not have enzymes‚ the reactions in our cells would be too slow to keep us alive. This means that they are extremely important for our survival. The properties of enzymes are: ⋆ They can be used many times‚ because they can speed up reactions without
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Table of Contents Abstract Introduction Aim Hypothesis Material Method Results Discussion Conclusion Abstract The aim of this experiment was to see whether different temperatures will affect the cell membrane‚ thus would then releases the purple pigments out of the vacuole which causes the leakage of the purplish liquid. Background Information The outermost layer is the cell wall‚ which is present only in plant cells and is made up of a carbohydrate called cellulose and also has
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Biofuel Enzyme Kit Katie Adamson Biochemistry Laboratory‚ BIO124L 1/29/15 Abstract The objective in this lab was to determine the effects different conditions had on the enzyme cellobiase. We examined reaction rates in the presence or absence of an enzyme‚ the effects temperature and pH changes on the enzyme and the effects enzyme concentration and substrate concentration had on the enzyme. As expected results showed us that cellobiase works optimally when conditions are favorable. We see this
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Explain the technique by which copper can be purified. What is a use of pure copper? 19) Explain the processes of phytomining and bioleaching. 20) Why is recycling beneficial to the environment? 21) What % of iron comes from the blast furnace? What effect does this have on its properties? 22) Give some properties of low-carbon‚ high-carbon and stainless steels. 23) Why might an alloy of aluminium be preferable to the pure metal in aeroplane manufacture? 24) What name is given to the metals in
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the R Lab 1. One mole of hydrogen gas has a mass of 2.02 g. Use your value of molar volume to calculate the mass of one Liter of hydrogen gas at STP. The density is in g/L. Compare your value to a literature value? (Consult a hand book or text book) 2. In setting up this experiment‚ a student noticed that a bubble of air leaked into the gas tube when it was inverted in the water bath. What effect would this have on the measured volume of hydrogen gas? Would the calculate value
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Laboratory 2: Scientific Methodology & Enzyme Activity Objective: The purpose of this experiment was to simply measure oxygen production rates released from decomposed hydrogen peroxide under different conditions (concentration of enzymes‚ temperature‚ and PH level). Hypothesis: Part a: If different amounts of enzyme solution are added to the hydrogen peroxide‚ then the highest amount of enzymes will have the greatest reaction rate because enzymes catalyze reactions‚ meaning more oxygen
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and measure the enzyme activity of β-galactosidase in the different concentrations of o-Nitrophenylgalactoside (ONPG) using a spectrophotometer. The spectrophotometer was also set at 420nm‚ a wavelength which is best for recording the absorbance values for the experiment. From the results‚ 0.9mM ONPG solution has the highest absorbance and 0.1mM ONPG solution has the least. Also‚ 0.5mM ONPG solution has the highest rate of enzyme activity and it is the most efficient as the enzyme activity of the
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HINTS ON WRITING YOUR REPORT Title: Enzyme activity Objective: To investigate the effect of temperature on amylase activity Design principle Background: Amylase activity products? (show the equation) Which factors will affect enzyme activity? How to study the rate of reaction? (e.g. rate of disappearance of substrates or rate of formation of products) Independent variable: temperature of reaction mixture or at which the enzymatic reaction occurs. It can be varied by setting water bath at
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however‚ without enzymes‚ these reactions could possibly take several years to complete. An enzyme is a macromolecule‚ generally a protein‚ that lowers the activation energy of a reaction without being changed by the reaction‚ and this causes the reaction to occur much faster than usual (Campbell et al.‚ 2014). The act of speeding of a chemical reaction is called catalysis‚ and molecules that perform this are called catalyst. Enzymes act as catalysts‚ and there a many types of enzymes that perform many
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Enzymes and ATP Enzymes act as protein catalysts in biochemical processes Enzymes bind to a substrate and forms the enzyme substrate complex. Enzymes work by lowering the energy of activation. Activation energy must be supplied for the reaction to begin‚ once supplied‚ the reaction can proceed on its own. Enzymes can speed up events. They are not used by during the reaction because the enzyme stays the same‚ it does not change during the reaction. (Hudon-Miller‚ Enzymes‚ 2013) Enzymes act as
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