Investigating the effects of temperature on cell membranes Independent variable: Temperature of beetroot Dependent variable: Absorbency of light Hypothesis: As temperature rises to its optimum temperature‚ the absorbency of light will increase because protein is an enzyme and will therefore be affected by temperature. This is because more beetroot dye will pass through the cell membrane and then into the distilled water. The deeper the colour of the beetroot dye‚ the higher the absorbency
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Table of Contents Abstract Introduction Aim Hypothesis Material Method Results Discussion Conclusion Abstract The aim of this experiment was to see whether different temperatures will affect the cell membrane‚ thus would then releases the purple pigments out of the vacuole which causes the leakage of the purplish liquid. Background Information The outermost layer is the cell wall‚ which is present only in plant cells and is made up of a carbohydrate called cellulose and also has
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Enzyme Activity Over Different Concentrations and Effects The goals of this experiment were to examine the effectively of enzymes on samples of different enzyme concentrations and substrate concentrations. In addition‚ the experiment tested how effective enzymes are on samples of pH levels and temperature levels. A. Effect of Enzyme Concentration Hypothesis: With half as much enzyme concentration then the reaction rate will be half as much than when the enzyme concentration is equal
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Investigation on Effects of Different pH on Enzyme Activity How does the different pH buffers affect activity of potato enzyme/extract? Introduction: Proteins are polymers that are made up of smaller units/monomers called amino acids. There are 20 different types of amino acids‚ thus make up many different combinations in types‚ numbers of amino acids as well as their orders – an explanantion for why there are so many proteins. Every protein‚ due to various reactions of amino acids to each
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College General Biology I Enzymes‚ test for effect of pH on catalase activity Purpose The main purpose of this experiment was to learn about enzymes and how to test for the effect of pH on catalase activity and to be able to tell if a reaction is an exergonic or endergonic process. Introduction Enzymes are made from amino acids‚ which are made from proteins. In order to make an enzyme‚ hundreds of amino acids are strung together in a very specific and unique order and eventually is folded
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prepared for the experiment‚ one at room temperature and the other two at 15°C and at 5°C. The room temperature water bath was prepared by filling a basin with tap water about ¾ full so that when the subject’s face is submerged‚ the water will not spill. A thermometer was used in order to obtain the temperature of the water. The temperature obtained was at 26°C. The water baths having temperatures of 15°C and 5°C were also prepared the same way as the room temperature water bath only with the addition of
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Abstract: This experimentation was to evaluate absorbance and the reaction rate of an enzyme‚ ’-amylase in starch-iodine solution. We will be testing the relationship between enzymatic reaction affected by temperature and pH. Through the testing the enzyme at different temperatures‚ and different pH levels; it would determine at which temperature and pH level the enzyme worked the most efficiently. Analyzing absorbance of the solutions with spectrophotometery will determine the reaction rate. To
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worn before starting the experiment. The initial room temperature should be calculated using a thermometer and a marker is used to name beakers 1 and 2. Divide the 10 test tubes into two groups and name them 1-5 in both groups. 5 different stopwatches are used to record the time for the disappearance of the blue-black color in test tubes‚ name them 1-5 also. Stopwatch 1 corresponds to test tube 1 in beaker 1 i.e. 0°C and the room temperature (that should be around 20-25°C)‚ and stopwatch 5 corresponds
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An investigation into the effects of sugar concentration on yeast activity Introduction: Yeasts are eukaryotic micro organisms belonging to the kingdom fungi. Yeasts live on sugars and produce ethanol and carbon dioxide as by-products. [James Mallory‚ 1984]When Yeasts are given water and sucrose they convert the sucrose into glucose then convert the glucose into carbon dioxide and ethanol following the following reaction: C₆H₁₂O₆ ( 2(C₂H₅OH + CO₂ [Brady Burkhart‚ Terrell Grayson and Eric Kimler
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Enzymes activity depends on the concentration of the substrate‚ temperature and the pH. The more concentrated the substrate is the more reactive the enzyme is. The optimal pH for an enzyme is 7.5 and the optimal temperature for an enzyme is 53 Celsius. Extremes in the temperature and the pH of an enzyme can denature therefore destroy it. The enzyme that is in this experiment is Amylase. Amylase is found in germinating seeds. These seeds contain starch in their endosperm. With the help of Amylase enzyme
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