Introduction: The purpose of this experiment is to measure the effects of changes in temperatures and pH on enzyme activity in skeletal muscle‚ particularly the activity of lactate dehydrogenase (LDH). LDH is a glycolytic enzyme which converts pyruvate to lactate in the following equation: LDH Pyruvate+ NADH ------------ Lactate + NAD The reaction above can move in both directions‚
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the bounded substrate. Amylase enzyme‚ just like any other enzyme‚ has an optimum PH and temperature range in which it is most active‚ and in which the substrate binds most easily. The purpose of this experiment was to determine (1) the reaction rate of an amylase enzyme in starch and (2) the environmental factors that can affect the enzymatic activity. The hypothesis‚ in relation to the enzymatic activity by variables such as the substrate concentrations‚ temperature‚ PH and chemical interactions
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pH Affecting on Amylase Enzyme Is there a difference in the concentration of pH in the amylase enzyme activity? The null hypothesis would be that there will be no difference in the concentration of pH and alternative hypothesis would be that there will be a difference in the concentration of pH. The predication if that there will be a difference in the concentration of pH. The pH affects on enzyme activities‚ one of the enzyme that was used in the process was amylase. When a serial dilution was
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Activity of Salivary Amylase Domingo‚ Guray‚ Hugo‚ Lorenzo‚ Mohammad Isa Intro Because everything has a start Catalysis The process of increasing the rate of reaction with the use of a catalyst. Catalyst – any substance that increases rate of reaction upon addition to a certain reaction Page 3 Enzymes Act on substrates in a reaction Highly specific Breaks down complex macromolecules‚ synthesizes compounds essential for the cell Active site Enzyme-substrate
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The Effect of Temperature on Animal and Fungal Amylase’s Ability to Breakdown Starch. Abstract This experiment was designed to test the reaction of the enzyme amylase at various temperatures. There were two different kinds of amylase being tested‚ one was fungal amylase also known as aspergillus oryzae and human amylase. The changes in temperature effect the rate at which an enzyme and a substrate collide. When the temperature is too high the active site changes shape or denatures‚ once this
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Lab Report The effect of temperature on the reaction between Catalase and H2O2 Sarah AlShemesi In this experiment we’ll be exploring the effects of temperature on the reaction between Catalase and H2O2.We’ll be using five different temperatures to test this. The five different temperatures will be 10‚ 30‚ 50‚ 70 and 90 oC. We will use the liver as a source of Catalase. A 1 gram piece of liver will be inserted into a test tube with 2 cm3 of water‚ then 2 cm3 of H2O2 will be added. The Catalase
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enzymes are found in living cells. In addition to making life possible‚ many enzymes have numerous applications that affect our daily lives in other ways such as food processing‚ clinical diagnoses‚ sewage treatment‚ and the textile industry. In this activity you will investigate how a particular factor influences the action of an enzyme. Conditions: Students are NOT required to design an experiment. Experimental design has been provided for you to use instead. You are however required to submit
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Temperature affecting enzyme activity Aim: To see how temperature can affect enzyme activity Prediction:-I think the one that will work best will be the rennin placed in milk at 37oC My independent variable will be the temperature My dependant variable will be how thick the milk becomes The equipment I need is milk‚ 5 test tubes‚ rennin‚ water bath‚ boiled rennin‚ a glass rod and a stopwatch Introduction: Rennin is an enzyme found in the stomach‚ its function is to solidify milk. We
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December 2012 1. Title and Author 1. The Effect of Extreme Temperatures on the Rate of Photosynthesis 2. Jeffrey Xia 2. Abstract A previous lab in which we conducted‚ tested whether or not the light intensity had an effect on the rate of photosynthesis. We concluded that light intensity did possess an effect on the rate of photosynthesis – the closer the experimental plant units were to the light source‚ the more experimental plant units exhibited the effect of gas exchange in photosynthesis.
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process can be affected by several variables; one of the most notable variables is the temperature of the plant’s environment. Previous study has led me to conclude that when the temperature of the plants’ environment is low‚ the rate of photosynthesis is also low because the enzymes responsible for photosynthesis are not able to absorb as much heat energy and in turn will move slower. In contrast‚ if the temperature of the plant’s environment increases‚ the rate of photosynthesis will also increase
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