The effect temperature has on the activity of the enzyme α- amylase. Introduction Enzymes are a biological catalysts‚ which means that they speed up the chemical reactions in living organisms. Almost all of enzymes are energized protein molecules that catalyse and regulate nearly all biochemical reactions that occur within the human body. The reason in which enzymes are sensitive to heat‚ pH and heavy metal ions is because they are made up of proteins. The food we eat is turned into energy by enzymes
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Investigate the effect of temperature on amylase activity Introduction Amylase is an enzyme that catalyses the breakdown of starch into sugars. Amylases are found in almost all plants‚ animals and microorganisms. Large amounts of amylase occur in germinating cereals‚ and in the pancreas and saliva of higher animals. Aim The aim of this experiment is to find out the rate of reaction between amylase and starch in a range of different reaction temperatures. Hypothesis As the reaction
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tested the effects of temperature on fungal amylase and bacterial amylase (Aspergillus oryzae and Bascillus Licheniformis). We used 4 different temperatures in Celsius 0‚ 23‚ 58‚ and 89 for both fungal and amylase. For 10 minutes‚ every 2 minutes we would use 3 drops of each amylase and mix it with iodine to observe the presence of starch at each temperature. We conducted this experiment for both bacterial and fungal amylase. Results were reached based on the color of the iodine and amylase mixture
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experiment to find the optimum temperature and pH for starch on amylase. The experiment was carried out in one day. The researcher and a partner did the experiment based on a lab manuel from class. Data was collected from the experiment and to be displayed on graphs. Then the optimum pH and temperatures were to be calculated based on the findings. The hypothesis was disproved due to the optimum pH of 5 but the other findings supported the hypothesis of the optimal temperature being 45 degrees Celsius.
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This study focuses on the effect that temperature has on the enzyme amylase. Enzymes are composed of proteins and acts as a catalyst to speed up the rate of a specific chemical reaction by lowering the activation energy. Reactions do not need enzymes to occur‚ but the human body and other living organisms depend on the use of enzymes in order for biochemical reactions to happen in milliseconds. Each enzyme contains a distinct three dimensional structure which is related to its particular function
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iodine can be measured by using a spectrophotometer. α-amylases are found in saliva‚ pancreatic juice‚ human breast milk‚ serum and certain tissues such as the liver. This enzyme catalyzes the hydrolysis of α (1-4) linkages in starch by breaking it down to maltose and some glucose. As the starch is broken down‚ the coiled structure of α-amylase is unfolded. Therefore‚ iodine will no longer be able to form the blue complex with the α-amylase. It can be assumed that the decrease in color (absorbance)
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Lab Report DIFFUSION AND THE EFFECT OF AMYLASE. Aim: We will interpret and explain the activity of an enzyme added to a solution. We will analyze reactions in different pH ranges. The starch solution will appear blue when mixed with iodine. But after mixing the enzyme amylase to the starch‚ the starches are broken down into simple sugars. Now‚ when the iodine is added‚ no color change exist. Instruments and materials used: 1.) Dialysis bags x2 2.)
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Abstract This experiment analyzes the effects of how temperature affects bacterial and fungus amylase and also discovers the optimum temperature for these enzymes. The amylase was mixed with starch at temperatures of 0℃‚ 37℃‚ 57℃‚ and 90℃. Iodine was added to each mixture and colour changes in each case. Bacteria amylase was found to be effective at 55 0C as the temperature dropped drastically from 4.58℃ to 2.33℃. This shows that the amylase catabolized a lot of starch hence little is left which
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enzyme called amylase. Amylase is the enzyme found in your saliva. The purpose of this enzyme is to help in the digestion process (McD‚ 2002). Enzymes are biological catalyst that help speed up the reaction by lowering the activation energy. These enzymes allow your food to be digested at a faster rate (National Institute of Asian Medicine‚ 2007). There are many factors that can affect the efficiency of enzymes which include pH levels‚ the amount of substrate‚ inhibitors‚ and temperature (Your Mom‚
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Determining the Optimal Temperature and PH of Barley Amylase Abstract The purpose of this experiment was to find the optimal temperature and pH of barley alpha-amylase. I hypothesize that the optimal temperature would be 55 degrees Celsius and the optimal pH would be 5.5. In this experiment‚ the starch is used as a substrate to examine the optimum temperature and pH for the reaction of alpha amylase. It is known that the measuring of disappearance (absorbance) of the substrate starch with iodine
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