Science investigation Aim: To try to see if the temperature affects the rate in which Amylase breaks down starch into maltose. In this reaction starch is the substrate and maltose is the product. Amylase is an enzyme‚ Enzymes‚ also called catalysts‚ are in living things and there are thousand of them. Enzymes break down food by the active site on the Enzyme forming a chemical bond with a substrate and then water attacks the substrate until it is hydrolysed (split in 2). Equipment: Boiling
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…………………KEY……………………… Matter & Energy Period …………. Skills 1. classifying types of matter 2. interpreting particle diagrams 3. identifying physical & chemical properties of matter 4. separating Mixtures 5. converting Temperatures 6. identifying physical & chemical changes in matter Skill #1: Classifying types of matter - refer to your notes and RB p. 1-2 Classify each of the following with the combination of terms listed below. pure substance – element mixture
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The effect of Lead ions on amylase activity Aim What is the effect of Lead ions on the enzyme Amylase. And does it have an inhibitory effect‚ which causes the substrate‚ in this case starch to be blocked from the reaction process in the enzyme catalyst. Also is the effect reversible or irreversible‚ which is put on the amylase. Method Apparatus and substances required Test tube holder 2% starch solution 6 boiling tubes labelled 1 to 6 1% lead nitrate solution 6 test tubes labelled A to E‚
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I predict that as I increase the temperature‚ the distance moved by the meniscus will also increase. I believe this will happen as aerobic respiration is taking place. This is respiration involving the consumption of oxygen gas‚ producing carbon dioxide and water‚ as shown below. C6H12O6 + 6O2 --> 6CO2 + 6H2O + 36ATP This process allows energy to be produced in the form of ATP‚ Adenosine Triose Phosphate. This occurs by converting glucose into pyruvate via glycolysis. Pyruvate is then transported
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8 1 EXPERIMENTAL DATA Table 1: Coordinate of Pressure Tapping Tapping No. 1 2 3 4 5 6 7 8 9 10 11 Note: Table 2: Pressure Readings Manometer inclination: Pressure Readings Pitot Pressure Static Pressure Atmospheric Pressure Atmospheric Temperature Stall angle: At the end of the experiment 474 mm 497 mm 500 mm 29°C (mm) 0.0 2.5 5.0 10 20 30 40 50 60 70 80 (mm) 0.000 3.268 4.443 5.853 7.172 7.502 7.254 6.617 5.704 4.580 3.279 0 0.025 0.049 0.098 0.197 0.295
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PLANNING COURSEWORK- STARCH AND AMYLASE AIM The aim of this coursework is to investigate the effect of temperature change‚ on the rate of hydrolysis of starch catalysed by amylase. PREDICTION I think that as the temperature increases‚ the rate of reaction also increases‚ to a point when it dramatically decreases. On graph 1‚ you will see a sketch of the graph which I expect to be the result of the experiment. SCIENCE REASONING I think my prediction is correct because the rate of
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10 mmol/L | Albumin | 30 g/L | Glucose | 12 mmol/L | Amylase | 5000 U/L --- Normal Range: 60-180 U/L | Serum: Comment: The diagnosis of acute pancreatitis is based on the clinical history‚ evidence of inflammation is known usually by computerized tomography (CT scan) and the finding of a high serum (or sometimes urinary) amylase activity. It is effectively a diagnosis of exclusion: the finding of a very high serum amylase activity is very suggestive but is not on its own diagnostic
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Enzymatic Activity of Salivary Amylase Abstract: Salivary amylase is an enzyme that can digest starch molecules and break them down to sugar molecules. In this experiment‚ the enzymatic activity and specificity of salivary amylase was examined depending on the changes in pH and temperature. In the first part of the experiment‚ the effect of temperature was determined‚ using constant temperature bath (4‚ room temp‚ 37‚ 50‚ 60‚ and 70°C). Having the room temp and 50°C as the highest and 37°C as infinite
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How Temperature Affects Amylase Activity Abstract This study of the effect of temperature on the enzyme amylase was performed to determine the relationship between the enzyme amylase and temperature. The rate of reaction was found to increase as the temperature of the environment was raised. As the temperature was raised from 5°C‚ 20°C‚ 35°C and finally to 80°C the rate of reaction followed this trend and also increased. However as predicted in the hypothesis of this experiment when the temperature
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Enzymatic Activity of Salivary Amylase Ong‚ Janela Rose I.; Paguia‚ Maria Tricia C.; Placente‚ Dax Daven A.; Posadas‚ Grace Catherine A. 3Bio3-Group 8 Department of Biological Sciences‚ College of Science University of Santo Tomas‚ España‚ Manila 1008 Abstract This experiment aims to examine the enzymatic activity and specificity of salivary amylase depending on the changes in pH and temperature; and determine the optimum temperature and pH of the amylase. EXPERIMENTAL In the
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