Studying the Rate of Reaction of catechol oxidase and how it affects pH levels Introduction: In this lab‚ we studied the activity of an enzyme that is found in fruits and vegetables called catecholase or catechol oxidase. An enzyme is a protein molecule that is a catalyst. Catechol oxidase is the enzyme in fruits and vegetables that turns them that undesirable brownish color; also commonly referred to as bruising or bruised. When walking through your regular grocery store and you find yourself in
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Why is your study significant‚ i.e.‚ how is your objective relevant/applicable to the larger world and why would the information you seek be important/valuable to others? (either in terms of using catechol oxidase as a model to study enzyme function or considering the specific activity of catechol oxidase) 3) State your hypothesis (an educated prediction): Materials and Methods 1) List specific materials used in your experiments (and sources of these materials). Also list any specialized
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Quinones are produced by the oxidation of phenolic compound of catechol. Enzymes are used to speed up chemical reactions in cells (Danyk‚ H‚ 2013). The enzyme catechol oxidase is used to speed up the production of benzoquinone which is to help in infection prevention. In this study catechol oxidase was combined with potato juice extract and water. To provide more results of the enzymes productivity patterns the solutions; temperatures were changed‚ alterations were made in pH‚ and the amount
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polyphenol oxidase serve as catalysts in the oxidation of several phenolic compounds. In this experiment however‚ the only reaction being tested is the oxidation of catechol (a diphenol) by the enzyme catalyst catechol oxidase or polyphenol oxidase‚ which initiates a chain of reactions and eventually triggers the formation of brown pigments known as catechol-melanins. Catechol‚ also called 1‚2-dihydroxybenzene is a phenolic compound with the chemical formula C6H4(OH)2. The catechol molecule consists
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Respectively‚ 1‚ 2‚ 4‚ 8‚ 16‚ and 24 drops of catechol were added to each of the tubes. To equal out the amount of solution in each of the test tubes‚ respectively 23‚ 22‚ 20‚ 16‚ 8 and 0 drops of pH 7 buffer were added. 30 drops of potato juice containing the catechol oxidase enzyme were then added to the solution‚ and then timed for 5 minutes at room temperature. The pH treatment only required 5 test tubes‚ with 3mL of matching buffer (pH4‚ pH6‚ pH7‚
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The Effect of Different Concentrations of the Enzyme Catechol Oxidase on the Rate of Benzoquinone Production When Mixed with Pure Catechol Carson Levine November 4th‚ 2013 Abstract Catechol oxidase is an enzyme that speeds up the oxidation reaction when catechol is exposed to oxygen. When the reaction occurs‚ benzoquinone is produced turning the oxidized substance brown. It was hypothesized that the higher the concentration of catechol oxidase‚ the browner the
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Lab: Inhibiting the Action of Catechol Oxidase By: Kimberly G. Introduction: In this lab‚ Mr. Greene ’s sixth period AP Biology class split into groups "to investigate inhibition of enzyme activity by specific chemicals called inhibitors" (1). Group three pondered this lab ’s inhibitor‚ phenylthiourea (PTU). Is it a competitive inhibitor? That is an inhibitor that literally "competes" with the substrate by mimicking it‚ and thus "wins" the position at the active site of the enzyme. The blocked
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Part B: Practical Report The Effect of Temperature on Enzyme Activity Aim: To investigate how temperature effects the enzyme catalase. Hypothesis: If the temperature of water is increased then the enzyme will react quicker to form oxygen and water‚ when compared to cold water. Purpose: To design and conduct a plan of a practical about the effects of temperature on enzymatic activity with a partner. Introduction: An enzyme is a protein‚ which speeds up a specific chemical reaction without altering
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extract in phosphate buffer pH6 containing the enzyme: polyphenol-oxidase‚ was used as the variable being tested in this experiment. You may refer to the Enzymatic Reactions Biology 21 Lab Manual. Santa Monica College by Logan‚ R. 2003 to see how the potatoes extract in phosphate buffer was made. We began this experiment by measuring seven constant amounts of 1ml of 0.1% catechol using a 1 mL pipet into each seven cuvettes. The catechol is our substrate solution. Next‚ different amounts of phosphate
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Investigating the Effect of Temperature on Enzyme Activity Almost all chemical reactions that occur in living organisms are catalyzed by enzymes. Many factors in a cell’s environment affect the action of an enzyme. In this investigation‚ you will design an experiment to determine the effect of temperature on an enzyme-catalyzed reaction. You will complete an entire lab write-up using the CHS LAB REPORT FORMAT. Fundamental Question How does temperature affect the rate of an enzyme-catalyzed
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