Paperose Lab- Introduction to Enzymes Ms. Kim/Honors Biology Purpose To Determine the rate of an enzyme reaction using a “modeled” enzyme and sugar‚ paperase and paperose. Introduction In this lab‚ your hands are the enzyme‚ paperase. This enzyme split the sugar‚ paperose‚ into subunit A and subunit B. You will split this molecule by ripping the paper model down the middle. Materials Paperose models‚ scissors‚ plastic bag‚ container (large plastic cup)‚ stopwatch‚ Procedure 1. Each
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PROCEDURE: Part A (Effect of temperature on growth) 1) 15 tubes of glucose broth are provided and one set of 3 tubes are inoculated with each of the following cultures; Escherichia coli‚ Pseudomonas fluorescens‚ Micrococcus luteus and Saccharomyces cerevisiae. The last served as control. 2) One of the three tube of each culture is incubated at each of the following temperature: * 4°C * 37°C * 55°C 3) All the tubes are incubated within 5 minutes after inoculating. The turbidity
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The effect temperature has on the activity of the enzyme α- amylase. Introduction Enzymes are a biological catalysts‚ which means that they speed up the chemical reactions in living organisms. Almost all of enzymes are energized protein molecules that catalyse and regulate nearly all biochemical reactions that occur within the human body. The reason in which enzymes are sensitive to heat‚ pH and heavy metal ions is because they are made up of proteins. The food we eat is turned into energy by enzymes
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The purpose of this experiment is to determine if the angle of impact affects the diameter and depth of a crater. The hypothesis was correct‚ stating that if the angle of impact changes than the depth and diameter will change due to the amount of speed and force is being put in the marble (meteor)‚ and the most effective angle for the diameter is at a 20 degree angle‚ and for depth is at a 90 degree angle. The hypothesis turned out to be true because it all depends to the angle the meteor or in this
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Temperature effects on the growth of microorganisms Introduction: The purpose of this experiment was to determine the effects that temperature has on three different organisms. Temperature is one of the most important environmental factors affecting growth and survival of microorganisms². The three organisms used where Escherichia coli‚ Pseudomonas fluorescens‚ and Bacillus stearothermophilis. Most bacteria grow within a particular temperature range. The minimum growth temperature is
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Enzyme Controlled Reactions 1) Describe the relationship between substrate concentration and the initial reaction rate of an enzyme-catalyzed reaction. Is this a linear relationship? What happens to the initial reaction rate as substrate concentration increases? A) The relationship between the substrate concentration and the initial reaction of an enzyme-catalyzed reaction is very productive‚ but is dramatically affected by the pH level of the given solution. The most productive pH level is
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I am choosing to study how the PSI (pounds per square inch) of a football is affected by the temperature. I chose to study this because I like football and I always pondered this question. I believe that this is a worthwhile question to study because these studies can help improve the game as the weather gets colder. As air cools‚ it expands‚ so when a football gets colder‚ the air inside it expands. This expansion causes the air pressure to decrease. The air pressure decreases because the molecules
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Introduction. The ’Stroop Effect ’ was first introduced in 1935. It originated from the theory of automatic processes. It is clear that some processing activities become automatic as a result of prolonged practice eg. Typing‚ driving‚ etc. Automatic processes therefore are fast‚ require no attention and are unavoidable. Stroop believed that there was some evidence that word identification may be a form of an automatic process. In the experiment participants had to name the colours in which the
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Practical: The effect of pH on enzyme activity Rationale Amylase is an enzyme involved in the digestion system which catalyses the breakdown of starch into sugars. It is not only present in human saliva but also in the pancreas‚ where it hydrolyses dietary starch into disaccharides and trisaccharides which are converted by other enzymes to glucose to supply the body with energy. pH has an effect on the activity of all enzymes‚ including amylase. As the pH level increases‚ the enzyme activity increases
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To prepare a temporary mount of aleaf peel to show stomata. B) Materials required:- Fresh leaves of plant‚ compound microscope‚ glass slides‚ cover slips‚ water‚ glycerine‚ safranine‚ blotting paper‚ needles‚ brush etc. C) Theory :- i) Stomata are minutepore present on the surface of the leaves. ii) Though they are found on both the upper and lower epidermis of the leaf‚ they are more in number on the lower epidermis. iii) Each stoma has two bean shaped
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