affecting the rate of catalase activity The extent at which environmental factors affect the rate of catalase activity was discovered in this lab. The assay system‚ in which a filter paper disc was dipped into the enzyme and submerged using a stirring rod in a test tube filled with 20mL of hydrogen peroxide‚ was used to test several enzyme factors. As the saturation of hydrogen peroxide increased the rate of reaction increased as well. When the enzyme concentration increased the rate of catalase
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error‚ he developed a process he called Appertizing. Fruits‚ vegetables‚ fish‚ or meat was sealed in stoppered bottles and the filled bottles were immersed in boiling water; the heat sterilized the bottles and food alike. Appert knew nothing of enzymes or bacteria‚ nor did anyone else in his day. He did observe that when food was heated in the sealed bottles‚ the food remained good as long as the seal was not broken. The food remained edible until the bottles were opened. The process won him
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Abstract: Enzymes are specific-type proteins that act as a catalyst by lowering the activation energy of a reaction. Each enzyme binds closely to the substrate; this greatly increases the reaction rate of the bounded substrate. Amylase enzyme‚ just like any other enzyme‚ has an optimum PH and temperature range in which it is most active‚ and in which the substrate binds most easily. The purpose of this experiment was to determine (1) the reaction rate of an amylase enzyme in starch and (2)
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As the temperature increased‚ the enzyme activity also increased‚ however‚ only to an optimal level which was at temperature 32oC. Once the temperature was beyond the optimal level‚ the rate began to decline and this was represented by the concave curve on figure 5 and 7. This was because as the temperature increases‚ the frequency of collisions between the enzyme and the substrate also increased hence faster reaction rate. Whereas‚ the enzymes operated slowly at low temperature as there wasn’t sufficient
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Laboratory 2: Scientific Methodology & Enzyme Activity Objective: The purpose of this experiment was to simply measure oxygen production rates released from decomposed hydrogen peroxide under different conditions (concentration of enzymes‚ temperature‚ and PH level). Hypothesis: Part a: If different amounts of enzyme solution are added to the hydrogen peroxide‚ then the highest amount of enzymes will have the greatest reaction rate because enzymes catalyze reactions‚ meaning more oxygen
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Temperature effects on the growth of microorganisms Introduction: The purpose of this experiment was to determine the effects that temperature has on three different organisms. Temperature is one of the most important environmental factors affecting growth and survival of microorganisms². The three organisms used where Escherichia coli‚ Pseudomonas fluorescens‚ and Bacillus stearothermophilis. Most bacteria grow within a particular temperature range. The minimum growth temperature is
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Effect of changes in substrate concentration on the reaction rate of an enzyme IB biology Internal Assessment 3/23/12 Research Question: Effect of changes in substrate concentration amount on the reaction rate of an enzyme Introduction: In this experiment‚ the substrate is hydrogen peroxide. The purpose of this investigation is to find out the relationship between the substrate concentration and the rate of reaction. Substrates are molecules that are acted upon by enzymes. For instance
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release an enzyme called amylase. Amylase is the enzyme found in your saliva. The purpose of this enzyme is to help in the digestion process (McD‚ 2002). Enzymes are biological catalyst that help speed up the reaction by lowering the activation energy. These enzymes allow your food to be digested at a faster rate (National Institute of Asian Medicine‚ 2007). There are many factors that can affect the efficiency of enzymes which include pH levels‚ the amount of substrate‚ inhibitors‚ and temperature (Your
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aim of this experiment was to determine the effect of caffeine on resting body temperature. This has to do with homeostasis‚ which is the maintenance of equilibrium. In other words‚ it is a stable body state. Homeostasis refers to the process of keeping the internal body environment in a steady state‚ when the external environment is changed (Homeostasis 2). Humans are warm-blooded creatures that generate body heat internally and maintain body temperatures at a fairly constant level – about 98.6oF
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carefully‚ as safety will be an issue throughout the whole experiment. We will wear goggles and an apron or lab coat to protect our eyes and clothes. As we are using enzymes and Hydrogen Peroxide we need to be extra careful‚ ensuring they don’t come into contact with our eyes‚ skin or clothes. Catalyse is an enzyme found in all living cells. It makes Hydrogen Peroxide decompose into water and Oxygen. We will be measuring the amount of Oxygen released from the Hydrogen Peroxide. In
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