THE EFFECT OF TEMPERATURE ON CATALASE ACTIVITY AIM The aim of this experiment is to find out the effect of temperature on catalase or hydrogen peroxide. This will enable us to tell at what temperature hydrogen peroxide is most efficient. This (degradation) reaction will help us determine some of the catalase’s different attributes. HYPOTHESIS In this experiment it would be safe to hypothesise that no activity would take place at 1 to 20 degrees. It would be probable that a little activity would
Premium Hydrogen peroxide Oxygen Temperature
Abstract In this lab‚ we tested the effects of temperature on fungal amylase and bacterial amylase (Aspergillus oryzae and Bascillus Licheniformis). We used 4 different temperatures in Celsius 0‚ 23‚ 58‚ and 89 for both fungal and amylase. For 10 minutes‚ every 2 minutes we would use 3 drops of each amylase and mix it with iodine to observe the presence of starch at each temperature. We conducted this experiment for both bacterial and fungal amylase. Results were reached based on the color of the
Premium Enzyme Starch Amylase
Investigating the effect of pH on amylase activity Aim The aim of the experiment is to determine the effects of different pH and the rate of reaction on fungal amylase and starch. Introduction The enzyme amylase is found in the human body‚ it catalyses the hydrolosis of internal glycosidic bonds in polysaccharides‚ the breakdown of starch into sugars. Amylase is present in human saliva‚ where it initiates the chemical process of digestion. Enzymes work best at an optimum pH of 7 which
Free Enzyme Starch Glucose
Introduction: Temperature - Is a measure of the motion of particles‚ increasing the temperature will cause the particles to move faster because the reactant particles have more thermal energy from the heat. When particles move faster‚ more frequent collisions will occur and the collisions are more violent. This should increase the reaction rate. This will result in the particles colliding more frequently and increasing the speed of the reaction. Also when they have more energy there will be more
Free Chemical reaction Chlorine Carbon dioxide
project is to demonstrate how water temperature affects the dissolving of sugar in liquid. Everything in our universe is made up of particles which are in constant motion. In a solid state particles move the slowest while in a liquid state particles move the fastest. Under the right conditions‚ solid particles (the solute) when mixed in liquid (the solvent) can form a solution. This occurrence is called dissolving. I wanted to answer the question; does the temperature of water affect the speed at which
Premium Sugar Solubility Temperature
clear. From this experiment I learnt that the bigger the size of the jelly is the more time its going to take for the colour change to take place‚ the colour change represents the rate of diffusion‚ the smaller the block of jelly is the less time its going to take for the colour change to take place which means the rate of diffusion
Premium Chemistry Ethanol Water
Lab #6: pH Effects and Enzyme Concentration on Catecholase Activity Abstract: This experiment examined the effect on how the changes in pH balance of potato juice affected the enzyme activity‚ structural stability and solubility. Introduction: Enzymes are macromolecules that allow chemical reactions to occur. They function as biological catalysts. A catalyst is a substance that is involved in‚ but not changed by or consumed in‚ a chemical reaction. The amount of energy required for a reaction
Premium Enzyme PH Chemical reaction
__________________________________ AP Biology LAB 2. ENZYME CATALYSIS ESSAY 2000 The effects of pH and temperature were studied for an enzyme-catalyzed reaction. The following results were obtained. a. How do (1) temperature and (2) pH affect the activity of this enzyme? In your answer‚ include a discussion of the relationship between the structure and the function of this enzyme‚ as well as a discussion of how structure and function of enzymes are affected by temperature and pH. b. Describe a controlled
Premium Allele DNA Experiment
LABORATORY REPORT Activity: Enzyme Activity Name: Angela Collins Instructor: Catherine Rice Date: 07.09.2014 Predictions Sucrase will have the greatest activity at pH 5 Sucrase will have the greatest activity at 70 °C (158 °F) Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity Dependent Variable amount of product (glucose and fructose) produced Independent Variable pH Controlled Variables temperature‚ amount of substrate
Premium Enzyme PH Temperature
EFFECT OF pH LEVEL ON THE ENZYME ACTIVITY OF INVERTASE John Dale Cruz‚ Michael Ray Dela Cruz‚ Karen Angelica Domalaon‚ Abegail Dulay‚ Shirley Ann Felipe Group 5 2C Medical Technology Biochemistry Laboratory ABSTRACT Invertase is a type of enzyme‚ a natural catalytic agent for biochemical reactions‚ can be obtained in Baker’s Yeast. Determination of the effect of pH on invertase activity is the primary objective of the experiment. Dinitrosalicyclic acid (DNS) Assay method is utilized to monitor
Free PH Buffer solution Enzyme