Investigate the effect of temperature on amylase activity Introduction Amylase is an enzyme that catalyses the breakdown of starch into sugars. Amylases are found in almost all plants‚ animals and microorganisms. Large amounts of amylase occur in germinating cereals‚ and in the pancreas and saliva of higher animals. Aim The aim of this experiment is to find out the rate of reaction between amylase and starch in a range of different reaction temperatures. Hypothesis As the reaction
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Osmosis is the spontaneous net movement of solvent molecules through a partially permeable membrane into a region of higher solute concentration‚ in the direction that tends to equalize the solute concentrations on the two sides. It may also be used to describe a physical process in which any solvent moves‚ without input of energy‚ across a semipermeable membrane separating two solutions of different concentrations. Although osmosis does not require input of energy‚ it does use kinetic energy and
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Hypothesis The optimum temperatures of Alcalase and Savinase will be different. Above and below their optimum temperatures activity will decrease. Biological explanation This investigation is designed to look at the effect of temperature on the activity of the proteases Alcalase and Savinase. By the end of it I hope to know the optimum temperature of both proteases. The substrate I am going to use during the experiments is the protein gelatin‚ which is a translucent‚ colourless‚ brittle solid
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Osmosis Osmosis is a special example of diffusion. It is the diffusion of water through a partially permeable membrane from a more dilute solution to a more concentrated solution – down the water potential gradient) Note: diffusion and osmosis are both passive‚ i.e. energy from ATP is not used. A partially permeable membrane is a barrier that permits the passage of some substances but not others; it allows the passage of the solvent molecules but not some of the larger solute molecules. Cell
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To investigate the effect of temperature on an enzyme controlled reaction Introduction and planning For the investigation of enzymes‚ I am going to conduct an experiment to see how temperature can affect the rate of reaction of enzymes by testing it with starch. The enzyme that we are going to use is called amylase. We are going to test this enzyme with starch. By mixing amylase and starch solutions together under different temperature conditions‚ we can record the rate of reaction by taking a
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experiment to find the optimum temperature and pH for starch on amylase. The experiment was carried out in one day. The researcher and a partner did the experiment based on a lab manuel from class. Data was collected from the experiment and to be displayed on graphs. Then the optimum pH and temperatures were to be calculated based on the findings. The hypothesis was disproved due to the optimum pH of 5 but the other findings supported the hypothesis of the optimal temperature being 45 degrees Celsius.
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The effect of different temperature on the cell respiration. Introduction： Cell respiration is the controlled release of energy from organic compounds in cells to form ATP‚and then release waste products. The process of cell respiration can take place in the presence or absence of oxygen. In either case‚the first stage of cell respiration is glycolysis.The effect of temperature on cell respiration mainly depends on the effect of temperature on of enzyme activity.Generally speaking in a certain
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An investigation into the effects of temperature on enzyme action An enzyme is a biological catalyst that speeds up the rate of reaction in certain biological functions. They play a vital role in many aspects of human physiology and are necessary for the functioning of a number of systems‚ for example in the digestive system to help to break down food. All enzymes have a unique active site that can fit on to a particular molecular arrangement on a target substrate; a substance e.g. carbohydrate
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INVESTIGATING THE EFFECT OF TEMPERATURE ON THE RATE OF ENZYME ACTIVITY. To investigate the effect that temperature has on enzyme activity I am going to use the enzyme amylase‚ which is used as a biological catalyst to break down starch‚ which cannot pass through the gut wall due to the size of the molecules‚ into smaller ones. Amylase is a carbohydrase‚ which converts starch to simple sugars in the Salivary Glands. Three features of all enzymes are: They are always proteins. They are specific
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Abstract In this lab‚ we tested the effects of temperature on fungal amylase and bacterial amylase (Aspergillus oryzae and Bascillus Licheniformis). We used 4 different temperatures in Celsius 0‚ 23‚ 58‚ and 89 for both fungal and amylase. For 10 minutes‚ every 2 minutes we would use 3 drops of each amylase and mix it with iodine to observe the presence of starch at each temperature. We conducted this experiment for both bacterial and fungal amylase. Results were reached based on the color of the
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