a convenient assay for this enzyme involves monitoring the change in absorbance of the reaction medium at 405 nm. Exergonic (i.e energy producing reactions) exhibit a negative free energy change. Sometimes these reactions occur spontaneously‚ but generally some energy must be supplied to initiate the reaction; in other word an energy barrier exists between the reactants and the products. The “energy barrier” represents the activation energy of a chemical reaction. In this practical the activation
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Effect of Temperature on Solubility of a Salt Ahmed Mohammed 17-nov-2013 Abstract In this experiment‚ you will study the effect of changing temperature on the amount of solutet will dissolve in a given amount of water. In this experiment‚ you will completely dissolve different quantities of potassium nitrate‚ KNO3‚ in the same volume of water at a high temperature. As each solution cools‚ you will monitor temperature using a computer-interfaced Temperature Probe and observe
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Enzymatic Reactions Enzymes are proteins found in living things that speed up chemical reactions. They aid in nearly all metabolic processes‚ such as food digestion‚ molecule synthesis‚ and the storage/ release of energy. An enzyme speeds up the rate of the chemical reactions by lowering the reaction’s activation energy‚ which means that by definition‚ an enzyme functions as biological catalyst. The activation energy is the energy that is used to get a reaction started. The function of an
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Enzymes are catalytic proteins that selectively speed up chemical reactions without by consumed by the reaction itself (1). Enzyme activity is significantly affected by factors such as temperature‚ substrate concentration‚ enzyme concentration and pH. Enzymes are highly specific and only catalyse one specific chemical reaction‚ speeding up the reaction by lowering the activation energy‚ the energy required to start a chemical reaction (2). At high temperatures and with various mechanical forces or
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Investigating the effect of temperature on plant cell membranes The purpose of this activity is: • to practise experimental and investigative skills • to investigate the effect of temperature on cell membrane structures Procedure SAFETY: Always carry scalpels clasped to a tile and with the tip pointing away from you. Beetroot cells contain pigments called betalains that give the tissue its dark purple-red colour. The pigment is contained in the cell vacuole. Investigation
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Melissa Carrithers AP Chemistry 12/17/14 Rate Law Determination Pre-Lab Purpose: The purpose of this lab is to observe the reaction between sodium hydroxide and crystal violet so that the order of the reaction could be determined along with the rate constant‚ k‚ and the half-life for this reaction. Hypothesis: If the reaction between crystal violet and sodium hydroxide reacts appropriately‚ then the order will be first order. Procedure: Mix 10 mL of sodium hydroxide and crystal violet solution
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What is Enzyme? Enzymes are protein that acts as speed up reactions and break down molecules in our body. However‚ different enzymes only work on certain types of molecules. Enzymes can accelerate the reactions by more than one million times.(3) In our human body‚ there are a total about forty thousand types of enzymes and each catalyzes different kind of molecule.(3) The molecules that enzymes help to accelerate is called substrates‚ and when enzyme is combined together with the substrate‚ it
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There are approximately 40‚000 enzymes living in one human cell‚ each responsible for a chemical reaction. Enzymes are complex 3D protein molecules created by amino acids‚ forming a unique sequence that produces hydrogen bonds‚ eventually formulating an enzyme within plants and animals (Boyle & Senior‚ 2002). Working alongside other molecules‚ they uphold a stable reaction system. The function of an enzyme is to aid and increase chemical reactions and organise metabolism‚ while maintaining homeostasis
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3 SUBJECT: Biology TITLE: Enzymes AIM: To investigate the effect of substrate concentration on the enzyme amylase INTRODUCTION: Enzymes are perhaps one of the most important proteins of the human body. Enzymes such as amylase‚ an enzyme that breaks down carbohydrates‚ work by means of surface catalysis. In other words‚ the surface of the enzyme enables other molecules to react in a manner they would not be able to without the surface of the enzyme present. Enzymes achieve this by lowering the
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Enzyme Lab Name ___________________________ Assignment 1: Getting to Know Enzyme Lab: Setting Up an Experiment The first screen that appears in Enzyme Lab shows you a biochemistry lab containing all the reagents and equipment you will need to perform your experiments. Click on each item in the lab to learn more about its purpose. Once you are familiar with the lab‚ click on the Experiment button to begin the first assignment. This assignment is designed to help you become
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