Broadly speaking‚ enzymes are proteins that is produced to perform as a biological catalyst in chemical reactions. Catalyst are used to increase the rate of a chemical reaction. In this study‚ we performed two different experiments that investigated the effect of varying substrate concentration‚ and the effect of temperature on the rate of Enzyme-Catalase reaction. In experiment one (i.e. the effect of varying substrate concentration on the rate of enzyme-catalase reaction) we tested the hypothesis
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Evaluation Like any experiment‚ there were a number of potential errors during the procedure of the experiment. Errors could have arisen as a result of the uncertainties associated with the instruments I used to take measurements‚ and also as a result of errors associated with the actual method. Of course‚ due to the limitations of the procedure‚ they could not be eliminated completely‚ so I will explain what I did to reduce them to an acceptable level and how I could have improved my method to
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January 2013 I. Title: Effect of a Catalyst on Reaction Rate II. Introduction: The basis of the experiment is the enzyme; an enzyme is a biological molecule that acts as a highly selective catalyst. By combining with a substrate‚ an enzyme is able to create a new product that helps the body function. (Ex. Lactose/Lactase) A substrate is a molecule an enzyme acts upon‚ the two combine at an area called an active site. This active site allows induced fit which allows the reaction to occur and a new product
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INVESTIGATING THE EFFECT OF TEMPERATURE ON THE RATE OF ENZYME ACTIVITY. To investigate the effect that temperature has on enzyme activity I am going to use the enzyme amylase‚ which is used as a biological catalyst to break down starch‚ which cannot pass through the gut wall due to the size of the molecules‚ into smaller ones. Amylase is a carbohydrase‚ which converts starch to simple sugars in the Salivary Glands. Three features of all enzymes are: They are always proteins. They are specific
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INTRODUCTION Enzymes are biological catalysts that speed up chemical reactions‚ without being used up or changed. Catalase is a globular protein molecule that is found in all living cells. A globular protein is a protein with its molecules curled up into a ’ball’ shape. All enzymes have an active site. This is where another molecule(s) can bind with the enzyme. This molecule is known as the substrate. When the substrate binds with the enzyme‚ a product is produced. Enzymes are specific to their
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Enzymes lower the amount of energy needed in a chemical reaction. This happens because enzymes are catalysts‚ so they speed up the activation rates that occur in living organisms. Without enzymes‚ it would be difficult to break down particles like food in the digestion system. Enzymes are all very specific to what chemical reactions they will work with‚ and the temperature‚ pH‚ and salt concentration have to be a specific levels in order for the enzyme to function. The structure of each enzyme has
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independent was the temperature of the water bath and the control was the deionised water and milk solution. These variables allowed for the experiment to be conducted accurately‚ with unexpected results. This is due to the enzymes being most active at 60ᵒC‚ it was that results were going to be most active at the 41ᵒC which is close to human body temperature. When we compare the enzyme in the low temperatures to the enzymes in the higher temperature we observed that the enzymes were functioning at
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An investigation into the effects of temperature on enzyme action An enzyme is a biological catalyst that speeds up the rate of reaction in certain biological functions. They play a vital role in many aspects of human physiology and are necessary for the functioning of a number of systems‚ for example in the digestive system to help to break down food. All enzymes have a unique active site that can fit on to a particular molecular arrangement on a target substrate; a substance e.g. carbohydrate
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that the enzyme concentration will affect the rate of reaction but only up until a certain point because there is a limited supply of substrate. Enzymes are protein molecule that acts as biological catalyst by increasing the rate of reactions without changing the overall process. They are long chain amino acids bound together by peptide bonds. Enzymes are seen in all living cells and controlling the metabolic processes in which they converted nutrients into energy and new cells. Enzymes also help
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Catalase Lab Introduction: Catalase is an enzyme normally found in many plant and animal tissues. Its purpose is to destroy toxic substances which may be introduced into cells. Also‚ some cells use catalase to destroy cellular debris or worn out organelles. In this lab‚ we will use a catalase solution from potatoes and determine the effect of temperature and pH on the action of this enzyme. The substrate of the enzyme will be 3% hydrogen peroxide (H2O2). Catalase works by the following mechanism:
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