The Effect of Concentration‚ pH and Temperature on Enzyme Activity Biology For Majors October 4‚ 2012 Abstract We examined the reaction an enzyme has when its concentration‚ pH and temperature are altered. In order to do this‚ we added different levels of pH into different test tubes with the enzyme (sucrose)‚ and substrate (sucrose)‚ and we then inverted the tube. The higher pH produced more enzyme activity. Temperature effects enzyme activity by decreasing its stability when the temperature
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A2 Practical Investigations Title: The effect of Temperature on the growth of Aspergillus oryzae Develop a Hypothesis This particular investigation is to discover how a range of temperatures effects the growth rate of the fungi Aspergillus oryzae. Most fungi’s tend to survive within the temperature range of 5-35oC‚ with the optimum depending on their normal environmental temperature. The fungi Aspergillus oryzae is heterotrophic which means they taken in their food from dead organic matter and cannot
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Practical: The Effect of Concentration on Reaction Rate Aim: To use a simple reaction between sodium thiosulphate and hydrochloric acid to discover concentration this determines how fast chemical reactions occur. Independent Variable: Concentration of hydrochloric acid (%) Dependent Variable: Time taken for chemical reaction to take place (sec) Hypothesis: My prediction is that the increased concentration of the thiosulfate will in turn lead to an increase in the rate of reaction. This
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Brooke Strowd AP Biology Ms. Butler 28 January 2013 I. Title: Effect of a Catalyst on Reaction Rate II. Introduction: The basis of the experiment is the enzyme; an enzyme is a biological molecule that acts as a highly selective catalyst. By combining with a substrate‚ an enzyme is able to create a new product that helps the body function. (Ex. Lactose/Lactase) A substrate is a molecule an enzyme acts upon‚ the two combine at an area called an active site. This active site allows induced fit
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initial rate of reaction. I will measure the effect of the enzyme in 5 different concentrations against the controlled variable of the reactant. The enzyme which will be used is different concentrations of potato and the reactant used will be Hydrogen Peroxide. Hydrogen Peroxide which will be the buffer solution is a PH of 7.2. My hypothesis for the experiment is that as the concentration of the enzyme is increased the rate of reaction will be increased‚ producing oxygen at a faster rate. The results
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Biochemistry CP2085 Effect of Temperature‚ Study and Measure of Enzymes Activity Abstract This experiment investigates the effect that temperature has on the rate of activity of enzyme β-galactosidase and also the rate of β-galactosidase activity in different concentration of substrate over time. Ο-nitrophenylgalactoside (ONPG) is used as a substrate for β-galactosidase. A spectrophotometer is used to detect the change in colour of the substrate. Results show that increase in temperature up to 50oC speeds
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Title of Experiment: The Effect of Temperature on the Hatching Success of Brine Shrimps. Abstract In this experiment‚ 40 brine shrimp eggs are put into a test tube containing salt water at different temperature such as 25°C and 35°C respectively. The eggs are left from two days to hatch. After two days‚ the number of eggs successfully hatched are observed and counted. Introduction Brine shrimp is the common name for a primordial crustacean that reaches less than 1.3 cm in length and is usually
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In class we decided that the rate of a reaction increases when there are more collisions or when the activation energy is reduced. We then determined that there were four ways to increase the reaction rate: increase temperature‚ add a catalyst‚ increase surface area‚ and increase the concentration of reactants. The objective of this lab was to experimentally show how temperature and surface area impact the rate of reaction‚ so only two of these methods were tested. The evidence indicates that increasing
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Effect of Temperature ( C ͦ) on Enzyme Catalase Activity in potato Aim: To investigate the Effect of temperature (10‚ 37‚ 60) Celsius (C ͦ) on enzyme catalase activity in potato using 2% of hydrogen peroxide (H202) as the substrate measuring the height (cm) of oxygen gas (bubbles) and calculating the volume of oxygen bubbles produced (cm3) Introduction: Enzymes are biological catalysts that speed up metabolic reactions without being affected. They lower the activation energy needed to start
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Aim:The aim of this practical is to investigate the effect of exercise on heart rate and breathing rate. We will use a digital heart rate monitor strapped on our chest while we perform different levels of exercise. A digital watch is also provided which receives signals from the heart rate monitor and displays your current heart rate on the screen. To measure the breathing rate at different levels of exercise‚ we measure the breathing rate before physical activity by counting the number of breaths
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