enzyme’s activity are temperature and pH‚ and each enzyme works best at a particular temperature and pH. The purpose of this enzyme kinetic experiment was to observe the effect of temperature and pH on the reaction of barley alpha-amylase enzyme with starch substrate and establish the optimum temperature and pH for this reaction. The optimum temperature and pH for the reaction of alpha-amylase and starch was predicted to be a temperature of 50°C and a pH of 5. The optimum temperature and pH for the reaction
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blue starch;I2(aq) + starch blue-black complex3. These three reactions react in a sequence. In any reaction the concentration is exponentially related to the rate of the reaction. The rate of the reaction is dependent on potassium iodate4. Therefore as the concentration increases‚ the rate of the reaction also increases. Since the rate of the reaction is inversely proportional to time‚ as the
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The effect of increasing substrate concentration on rate of an enzyme reaction. Enzymes are biological catalysts that lower a reactions activation energy making possible many of the reactions needed for life to exist. Enzymes have a high specificity which have been explained by many theories such as Fischer’s lock and key. Currently the most widely accepted theory is the induced fit hypothesis proposed by Koshland in 1958. This hypothesis solves some of the problems with the Lock and key theory
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have different demands in the current industry. The reaction rate of the reaction plays an important role in the production of the ester which could affect the production of the ester. Esterification is a reversible reaction and thus this affects the amount of ester produced as ester hydrolysis changes it back to its reactants. Very little data is available on how does the carbon chain length of carboxylic acid and alcohol affects the rate constant and equilibrium constant of esterification. Thus‚
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Resting metabolic rate can vary with age independently from body mass changes in the Colorado potato beetle‚ Leptinotarsa decemlineata. Abstract A study was done by Saija Pironine‚ leena Lindstrom‚ and Anne Lyytinen in the Journal of Insect Physiology‚ to investigate the age dependence of resting metabolic rate in Colorado potato beetle (Leptinotarsadecemlineata) from adult emergence until age 10 days. In their hypothesis they expected to see a bell shaped pattern in metabolic rate with adult age
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The effect of enzyme concentration‚ substrate concentration‚ pH‚ and temperature on the enzyme catalase. Introduction: Enzymes are biological catalysts; proteins and RNA. They are required for most biological reactions and they are highly specific. Each enzyme has an active site. The active site is the spot on the enzyme where a substrate fits in. Substrates binds with enzymes through the active site. Enzymes‚ being highly specific‚ only fit with one certain substrate. Enzymes and substrates
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When a beetroot cell is exposed to various kinds of temperatures‚ its plasma membrane is affected and may change in structure resulting in the leaking of betacyanin. If the temperature changes‚ then I would expect to observe that there would be a change in the concentration of betacyanin that has leaked from a beetroot cell. In specific‚ at high temperatures‚ the phospholipids that make up the plasma membrane of the beet root cell become progressively more destabilized causing them to transform into
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Appreciate how a physical property that varies with temperature may be used for the measurement of temperature‚ and state examples of such properties. Recognise the need for and identify fixed points. Describe the structure and action of liquid-in-glass thermometers. Demonstrate understanding of sensitivity‚ range and linearity. Describe the structure of a thermocouple and show understanding of its use for measuring high temperatures and those that vary rapidly. 1. A clinical thermometer
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2. How is the colour related to surface temperature of the star? The correlation between the surface temperature of a star and its colour is due to the phenomena known as black body radiation .A star is a black body‚where it aborbs all the radiation falling upon it.AS its temperatures gets hotter thanits surrounding‚the star radiates its on electromagnetic radiation‚known as black body radiation. This radiation is distributed unevenly but contionously through multiple wavelengths of the electromagnetic
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The Correlation between the Diffusion Rate of a Substance and its Molecular Weight ABSTRACT To test the effect of molecular weight on the rate of diffusion‚ various experiments were performed. One of which is the glass tube test wherein cotton balls of the same size were moistened in two different substances (NH4OH and HCl). These cotton balls were plugged at each side of a glass tube. After some time‚ formation of a white ring occurred. The white ring‚ in fact‚ is a product of the reaction between
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