the bacterium was a gram negative or a gram positive. After performing the gram stain‚ I concluded that by the appearance of purple spherical clusters resembling grapes that is was a gram positive cocci. A Catalase test was then performed using hydrogen peroxide. A positive catalase test was observed when bubbles started to form on the culture distinguishing Staphylococcus spp. form on the culture distinguishing Staphylococcus spp. from Streptococcus spp. Finally‚ a Mannitol Salt Agar
Premium Bacteria Microbiology Gram staining
Catalase Enzyme Lab Samiya Hussein March 9‚ 2012 Introduction In order to receive the necessary amounts of energy required for daily function‚ the digestive system must break down proteins‚ fats and carbohydrates. In doing so‚ the body produces poisonous chemicals; however‚ the cells aren’t harmed. This is because enzymes are used to break down these chemicals. The name of the enzyme that was the main focus of the lab is catalase. Catalase is responsible for catalyzing hydrogen peroxide
Premium PH Enzyme Hydrogen peroxide
known as catalase facilitates this decomposition reaction. The catalase enzyme acts as catalysis‚ helping lower the energy needed to activate the reaction while the enzyme itself is not affected. Catalase is a digestive enzyme used to break down hydrogen peroxide‚ which is a normal byproduct of cellular respiration. The reaction could take place without the help of catalase‚ but it would happen a lot more slowly because more energy is needed for the reaction. This lab demonstrates the effect the enzyme
Premium Oxygen Hydrogen peroxide Enzyme
Introduction: Catalase is an enzyme‚ which is present in all life forms that utilize oxygen for their biological processes. Enzymes are proteins which increase the rate of reaction by decreasing the activation energy (the energy required to initiate a reaction). One of the main function of Catalase is to prevent the accumulation of hydrogen peroxide (H2O2) in the body by breaking it down into water and oxygen gas. Hydrogen peroxide is a toxic by-product of metabolic reactions. If hydrogen peroxide
Premium Enzyme Metabolism Oxygen
Carbonates react with strong acids to give off carbon dioxide and water. Marble is calcium carbonate and thus behaves in the same way: CaCO3 (s) + 2HCL (aq)  CaCL2 (aq) + H2O (l) +CO2 (g) In this experiment‚ I am going to see if temperature affects the reaction rate between marble chips and hydrochloric acid by timing the release of carbon dioxide in the reaction. I predict the higher the temperature‚ the faster the reaction rate. This is due to the kinetic theory. The more heat
Premium Calcium carbonate Carbon dioxide Chemical reaction
[Name] [Teacher] [Course] [Date] Catalase Lab Report An enzyme is something that helps to speed up a chemical reaction. The enzyme changes from reaction to reaction‚ but it always has the same impact. However‚ certain variables may cause the enzyme to have a more or less significant impact on the speed of each reaction. One of these variables that changes the effectiveness of an enzyme is temperature. There is an optimal functioning temperature for each enzyme in each reaction‚ depending on
Premium Chemical reaction Oxygen Water
Investigation of Action of Saliva and 3 M Hydrochloric Acid in Two Carbohydrate Solutions Title : Investigation of Action of Saliva and 3 M Hydrochloric Acid in Two Carbohydrate Solutions Objective : To investigate the action of saliva and 3 hydrochloric acid in two carbohydrate solution Results Table1: Observation Conclusion Solution A Benedict’s test: Blue coloration turned to brick red precipitate. Reducing sugar is present in the solution A. Iodine test: The coloration remained
Premium Starch Enzyme Glucose
Title : Investigation of Action of Saliva and 3 M Hydrochloric Acid in Two Carbohydrate Solutions Objective : To investigate the action of saliva and 3 hydrochloric acid in two carbohydrate solution Results Table1: Observation Conclusion Solution A Benedict’s test: Blue coloration turned to brick red precipitate. Reducing sugar is present in the solution A. Iodine test: The coloration remained unchanged. Starch is absent in solution A. Solution B Benedict’s test: The blue coloration
Premium Starch Enzyme Amylase
Aim: To investigate the effect of hydrogen peroxide concentration on enzyme catalase Indroduction: Enzymes are proteins. They function as biological catalysts. They lower the energy barrier of a reaction so that the reaction can take place at body temperature. Also‚ they can speed up Metabolic reactions without being changed or used up. During a reaction‚ an enzyme molecule combines temporarily with the substrate. When the reaction is complete‚ the enzyme molecules returns to its original dorm
Premium Enzyme Oxygen Catalase
Title: Catalase enzyme detection Objective: To understand the function of catalase in cells that produce the enzyme‚ interpret the results of a catalase test and know their value in differentiating bacteria. Materials: 1 clean microscopic slide‚ 3% H2O2 solution‚ swabs. Micrococcus luteus‚ Enterococcus faecalis‚ patient G Procedure: 1) Scrape some cells off from each bateria to the slant and place them on glass slide. 2) Place one or two drops of H2O2. Watch for bubbling as an indication of
Premium Catalase Bacteria Hydrogen peroxide