The acid-fast stain is performed on samples to demonstrate the characteristic of acid fastness in certain bacteria. Acid fastness is a characteristic that is shared by just a few organisms‚ so staining to determine if organisms possess this trait is useful in microbial identification schemes. The Ziehl-Neelsen method has endured as a reliable and effective way to demonstrate the acid-fast bacteria. Materials: 18-20 nutrient hour agar slant culture of Staphyloccus aureus 4 day old nutrient
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Abstract The objective of this experiment was to observe multi-step purification of benzoic acid after performing the extraction from a mixture containing benzoic acid‚ cellulose‚ and methyl orange. Recrystallization was done to remove impurities from the sample. The amount of BA recovered during recrystallization is much less than the extracted amount of BA. The difference between the pure and impure samples was observed by comparison of melting points. It was found that impure samples will have
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Part A: Table 1: Fractionation of amino acid Fraction No | Response to Ninhydrin test(level of blue colour change) | Response to Sakaguchi test (colour change) | 1 | X | yellow | 2 | Dark blue | yellow | 3 | Dark blue | Pale orange | 4 | Blue | Pale orange | 5 | Pale blue | Pale orange | 6 | Blue | Pale orange | 7 | Pale blue | Pale orange | Discussion Part A In the separation and purification of a single protein or amino acids‚ a solution containing the desired analyte
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Unknown #: 146 Fall-11 Organic Chemistry Midterm Report 13 October 2011 Table of Contents Separation of Benzoic Acid and Acetanilide 3 Extraction and Purification of Caffeine from Tea 4 Extraction of Trimyristin from Nutmeg 5 Conversion of t-butanol to t-butyl chloride 6 Appendix 7 Calculations 8 Literature and Experimental Values of Benzoic Acid and Acetanilide 9 Experimental Caffeine IR 10 Literature Caffeine IR 11 Experimental Trimyristin IR
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Exercise 10 Acid/Base Balance NAME 1. Match each of the definitions in Column A with the appropriate description in Column B. Column A Column B F 1. pH E 2. acid D 3. base A 4. acidosis B 5. alkalosis C 6. carbon dioxide a. condition in which the human body’s pH levels fall below 7.35 b. condition in which the human body’s pH levels rise above 7.45 c. mixes with water in the blood to form carbonic acid d. substance
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Alanine (ala‚ A) (neutral‚ non-polar) Arginine (arg‚ R) (basic‚ polar) Asparagine (asn‚ N) (neutral‚ polar) Aspartic Acid (asp‚ D) (acidic‚ polar) Cysteine (cys‚ C) (neutral‚ slightly polar) Glutamic Acid (glu‚ E) (acidic‚ polar) Glutamine (gln‚ Q) (neutral‚ polar) Glycine (gly‚ G) (neutral‚ non-polar) Histidine (his‚ H) (basic‚ polar) Isoleucine (ile‚ I) (neutral‚ non-polar) Leucine (leu‚ L) (neutral‚ non-polar) Lysine (lys‚ K) (basic‚ polar) Methionine (met‚
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Titration for Acetic Acid in Vinegar Purpose: The Primary Purpose of this Experiment is to Determine the Percent Content of Acetic Acid in a Household Bottle of President’s Choice Vinegar‚ using Titration Techniques. In addition‚ a Secondary Purpose for this Experiment that Derives Directly from the Primary Purpose is to Gain Hands On Experience in Titration Techniques‚ as a Vital Tool in our Quest to Understanding the Chemical Aspect of the World around Us. Procedure Conducting a Titration
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Acetic acid (glacial) 100% suitable for use as excipient EMPROVE® exp Ph Eur‚BP‚JP‚USP‚E 260 For general questions please contact our Customer Service: Merck KGaA Frankfurter Str. 250 64293 Darmstadt Germany Phone: +49 6151 72-0 Fax: +49 6151 72 2000 01 March 2014 Product number Packaging Qty/Pk 1000562500 Glass bottle 2.5 l 1000569025 Plastic container 25 l 1000569190 Plastic barrel 190 l Accessories 101595 Chemizorb® H+ Absorbent and neutralizer for spilled acids‚ with indicator
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necessary reaction schemes. The reaction schemes should include molecular weights and any other important physical data for the reagents and products. Ex. Cyclohexanol was oxidized to cyclohexanone using sodium dichromate in the presence of sulfuric acid. * Note: you can download a free version of a structure editor from ACD labs‚ the PC version is called chemsketch‚ the Mac version is called marvinsketch. You should learn how to draw structures using these programs. Lab reports should look professional
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sodium thiosulphate (thio) and hydrochloric acid. Hydrochloric Acid + Sodium Thiosulphate "" sodium chloride + water + sulphur The reactants are both colourless‚ but one of the products‚ sulphur‚ is yellow‚ so we can use this fact to follow the rates of reaction. I am going to investigate how the concentration of the acid effects the speed of the reaction. Apparatus 1 small measuring cylinder 1 large measuring cylinder 1 small beaker 1 large beaker Hydrochloric Acid Sodium Thiosulphate Cross
Free Chemical reaction Chlorine Sodium chloride