- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Effect of Substrate Concentration on Catalase Aim: An experiment to find out how the concentration of hydrogen peroxide affects the rate of reaction of the enzyme catalase. Background Information: Enzymes such as catalase are globular protein molecules with catalytic properties. A catalyst is a substrate which can alter the rate of reaction without itself undergoing any permanent change. As they are not changed
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hydrogen -A decrease in the oxidation state Objective: To find the redox reactions between a metal and dilute acid‚ metal-metal ion displacement and halogen displacement reaction. Materials: Test tubes (8)‚ pea size samples of the following metals; copper‚ lead‚ magnesium and zinc (10 ml)‚ 2M hydrochloric
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Title: Enzyme Catalysis of Hydrogen Peroxide by Catalase Problem and Objectives: How do different temperatures and different levels of pH affect the reaction rate of the enzymes in chicken liver? Demonstrate the activity of an enzyme in living tissue‚ observe the effects of changes in temperature and pH on the activity of an enzyme‚ perform analyses for the presence of an enzyme in tissues‚ and analyzing relationships between environmental conditions and enzyme activity. Background: Cells produce
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Title: Investigation of action of saliva and hydrochloric acid in two carbohydrate solutions. Objective: To determine the action of amylase and hydrochloric acid in two carbohydrates solutions Apparatus and equipments: 1. Boiling tube 2. Metal test tube racks 3. Beaker 4. Graduated plastic dropper 5. Water bath‚ ~37ᵒC 6. Water bath‚ ~95ᵒC Materials: 1. Carbohydrate solution A 2. Carbohydrate solution B 3. Benedict’s solution 4. 3M Hydrochloric acid 5. 3M Sodium hydroxide Procedures: 1. Two
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Practical 3 Investigation of Action of Saliva and Hydrochloric Acid in Two Carbohydrate Solution | Objective: 1. To show the action of saliva in two carbohydrate solutions. 2. To show the action of hydrochloric acid in two carbohydrate solutions. Apparatus & Equipment’s: Boiling tubes Metal test tube racks Beaker Graduated plastic dropper Water bath‚~37°C Water bath‚~95°C Stop watch
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Abstract The purpose of this lab was to test if ethanol affects the reaction involving hydrogen peroxide and catalase. Tests were performed by putting chicken liver‚ ethanol solution (diluted ethanol solution for other trials) and hydrogen peroxide in a test tube with a side arm‚ and having a rubber tube lead the oxygen gas into a gas collection tube. Results from the tests showed a negative correlation‚ this means that the more diluted the solution of 95% ethanol was‚ the less oxygen gas collected
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the optimum pH for catalase in potatoes. This is because the height of the bubbles was the highest of all three tests (pH 3‚ pH 7‚ pH 9) reaching an average height of 0.433 cm. See graph 3. While pH buffer 3’s average height was 0.233 cm only reaching a maximum height of 0.3 cm in Trial 3 and pH buffer 9’s average height being 0.333 cm only reaching a maximum height of 0.4 cm again in Trial 3. See table 1. Therefore this supports my hypothesis: At pH buffer 7 the enzyme (catalase) will work most efficiently
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Experiment 4: Analyze a solution of potassium hydroxide using standard hydrochloric acid Theory . Introduction: Titration is a procedure used in chemistry in order to determines the molarity of an acid or a base. In the other words‚ it is also consider as acid-base neutralization reaction (Darrell D. Ebbing 1976). A chemical reaction is set up between a know volume of a solution of unknown concentration and a known volume of a solution with a known concentration. The relative acidity or
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Abstract In this laboratory exercise we studied enzyme catalase‚ which accelerates the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase and measure the rate of activity under different conditions. The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes. Changes in temperature and pH along with Substrate Concentration and Enzyme Concentration were the conditions tested in the
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Finding the Activation Energy of the reaction between Hydrochloric Acid and Sodium Thiosulfate The equation for the reaction is: S2O32- (aq) + 2H+(aq) ⋄ SO2 (g) + S(s) + H2O (l) Equipment - 2 boiling tubes - 400 cm3 beakers - Marker pen - Stand and clamp - Timer - Bunsen burner‚ tripod and gauze - 0 – 100 oC thermometer - 2 x 10 cm3 measuring cylinders - Access to a fume cupboard. Method 1. Label two boiling tubes A and B. Mark a dark spot on the side of a 400cm3 beaker‚ then ½ fill
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