Catalase Lab Purpose: To observe the effects of hydrogen peroxide on living tissue and non-living materials‚ showing the presence or absence of catalase as observed by the presences of bubbles. Hypothesis: If we pour H2O2 on certain substances‚ the catalase will break down and bubbles will appear at the surface. Data table: Name | Time | Observation | Raw Potato | 1 min. 30 sec. | Slow reaction‚ barley any bubbles. | Raw Liver | 5 min. 30 sec. | Extremely fast reaction‚ very foamy‚ Big
Premium Oxygen Hydrogen peroxide Enzyme
MEASURING pH Date of Lab: 02/09/13 Date Report submitted: 02/26/13 Purpose of the experiment By doing this experiment‚ we were trying to learn how to measure a pH with a pH meter to determine whether a solution is weakly‚ moderately or strongly acidic or basic; but also to compare the different data founded to see how the addition of certain liquid to a solution may affect the pH of the initial solution. Materials We used: * Tap water‚ distilled water‚ Soda and milk as the main
Premium PH Acid Water
Arnesh Kaewloyma 9/30/14 Enzyme Catalase under optimum conditions‚ speeds up the decomposition of hydrogen peroxide The students have studied that Hydrogen peroxide is a jeopardy to metabolism if it’s not obliterated. Enzymes are proteins produced by living cells of that act as catalysts which affects the rate of biochemical reaction by speeds up the breakdown of hydrogen peroxide into water and oxygen gas. The students have formed a hypothesis toward this experiment. If the speed of
Premium Hydrogen peroxide
by a cold catalase solution and the other in which the Hydrogen peroxide(H2O2) was denatured by a hot catalase solution. The experiment conducted displayed signs of experimental error as there were many situations in which the materials provided could have faulted. Theoretically speaking‚ from the inconsistent values provided for the pH levels‚ a possible error could be that the pH probe may have been internally damaged such that the filling solution was contaminated. Moreover‚ the pH probe although
Premium Chemistry Oxygen Hydrogen peroxide
Experiment 20: pH Titration: Phosphoric Acid in Cola Drinks Post-lab Assignment or Report The post-lab report for this experiment is due at the beginning of the following lab period. Student notes for the lab will be available on the lab T-Square site. Learning Objectives Students will be able to... • Use a known mass of solid acid to determine an unknown concentration of a basic solution (this process is called “standardization”). • Execute a titration using good‚ reliable technique.
Premium Acid PH Titration
How different concentrations of hydrogen peroxide would affect the activity of catalase? 1. Variables Independent Concentration of hydrogen peroxide Dependent Volume of oxygen gas evolved Control Amount of liver used Location of liver sample obtained from the liver Type of liver used Possible enzymes transferred from hands during making liver homogenate Deterioration of liver Endpoint identification Volume of hydrogen peroxide added Reading on the syringe Room temperature
Premium
Question 1 Critique Dr. Honeydew’s report. Specifically‚ discuss what is missing and how the missing information should have been presented. First the PH measurements‚ Dr. honey dew does not explain how the PH device was calibrated or if the blood sample was added or mixed with anything prior to the measuring of the pH (like water‚ etc.). In regards to the absorption spectroscopy‚ the method by which the concentration was obtained is questionable. Was the concentration known before the experiment
Premium PH
trend O2 production and reaction velocity increased with increasing catalase concentration‚ however‚ the 33% percent catalase concentration showed a drop of 0.175 mL O2/s compared to the 25% catalase concentration (figure 1.2). The velocity of 25% catalase was 0.275 mL/s‚ 33% was 0.1 mL/s‚ 50% was 0.435 mL/s‚ and 75% catalase was 0.575 mL/s (figure 1.1). The 50% catalase concentration produced the most O2 overall however the 75% catalase concentration had the fastest initial reaction velocity. Experiment
Premium Enzyme Chemical reaction Chemical kinetics
EFFECT OF TEMPERATURE ON THE RATE OF CATALASE REACTION IN HYDROGEN PEROXIDE INTRODUCTION Enzymes function similarly to a lock a key mechanism where only one specific key will work (U and Fischer‚ 1994). An enzyme is a macromolecule that acts to increase the rate of either a substrate breaking down into two or more products‚ or where two substrates join up to form one product (Dundon‚ 2018). These enzymes perform this by lowering the activation energy required for the reaction to occur under normal
Premium Oxygen Enzyme Hydrogen peroxide
reactions. One of them is pH‚ which influence on the enzyme activity will be the subject of this investigation. Catalase of potato tuber cells will be the enzyme used in the experiment. RESEARCH QUESTIONWhat is the influence of different values of pH on the activity of catalase in potato tuber cells?VARIABLESIndependent: potato discs treatment (placing them into buffer solutions of different pH values‚ mixed with hydrogen peroxide)Dependent: the activity of the catalase‚ reflected by the rate at
Premium Enzyme Metabolism Catalysis