Title: The affects of hydrogen peroxide on catalase reactions in animal and plant cells at different temperatures and states. Introduction: All living organisms in the kingdoms of life are composed of and depend on cells to function normally. Not all cells‚ however‚ are alike. There are two primary types of cells: eukaryotic and prokaryotic cells. Cells contain organelles‚ or tiny cellular structures‚ that carry out specific functions necessary for normal cellular operation. (Regina Bailey Updated
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Hypothesis The optimum temperatures of Alcalase and Savinase will be different. Above and below their optimum temperatures activity will decrease. Biological explanation This investigation is designed to look at the effect of temperature on the activity of the proteases Alcalase and Savinase. By the end of it I hope to know the optimum temperature of both proteases. The substrate I am going to use during the experiments is the protein gelatin‚ which is a translucent‚ colourless‚ brittle solid
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Investigate the effect of temperature on amylase activity Introduction Amylase is an enzyme that catalyses the breakdown of starch into sugars. Amylases are found in almost all plants‚ animals and microorganisms. Large amounts of amylase occur in germinating cereals‚ and in the pancreas and saliva of higher animals. Aim The aim of this experiment is to find out the rate of reaction between amylase and starch in a range of different reaction temperatures. Hypothesis As the reaction
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Reaction of catalase with hydrogen peroxide AIM: I aim to find the rate of reaction between catalase and hydrogen peroxide. Enzymes such as Catalase are protein molecules that are found in living cells. They are used to speed up specific reactions in the cells. Each enzyme just performs one particular reaction so they are all very specific. Catalase enzymes found in living cells e.g. in yeast‚ potato or liver‚ speed up (in our case) the breaking down of hydrogen peroxide. The lock and key analogy…
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The Effect of Temperature on Enzyme Activity and Oxygen Production Throughout this report you will gain information as to how temperature effects the amount of oxygen produced in an enzyme- catalase experiment. In the experiment we used liver extract as a catalase and created a chemical reaction within a reaction chamber between the catalase and hydrogen peroxide as well as three different controlled temperatures. In the procedure below there will be a step by step process as to how
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all. This is why enzymes are crucial to life‚ without it life would not exist on earth. One of the many enzymes that are produced naturally within the body is catalase. In Denham Harman’s free-radical theory of aging‚ it is theorized that cells age as a result of exposure to free radicals and oxidizing agent such as hydrogen peroxide. Catalase is the major line of defense that converts hydrogen peroxide into water and oxygen‚ thus decreasing levels of free radicals in the body. According to an article
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Substrate concentration and yeast catalase Aim: To see how the substrate concentration in hydrogen peroxide affects the rate of an enzyme controlled reaction using yeast catalase. Introduction: An enzyme is a biological catalyst made of protein. Enzymes are protein molecules found in living organisms and in this case I will use a yeast catalase. Catalase is an enzyme that catalyzes the reduction of hydrogen peroxide. Hydrogen peroxide is a poisonous by-product of metabolism‚ so it is very
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INVESTIGATING THE EFFECT OF TEMPERATURE ON THE RATE OF ENZYME ACTIVITY. To investigate the effect that temperature has on enzyme activity I am going to use the enzyme amylase‚ which is used as a biological catalyst to break down starch‚ which cannot pass through the gut wall due to the size of the molecules‚ into smaller ones. Amylase is a carbohydrase‚ which converts starch to simple sugars in the Salivary Glands. Three features of all enzymes are: They are always proteins. They are specific
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A Quantitative Enzyme Study: CATALASE FlowChart Purpose: Measure the rate of enzyme activity in different conditions Procedure: A. Extraction of Catalase 1. Peel potato 2. Cut into cubes 3. Mass 50g 4. Measure out 50ml of cold distilled water in blender 5. Add crushed ice into blender (small amount) 6. Add the potato cubes into the blender 7. Turn on blender on high for 30 seconds 8. Prepare an ice bath - FROM THIS POINT ON PREPERATION MUST BE CARRIED OUT IN AN ICE BATH – 9
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ESTIMATION OF MAJOR TISSUE CATALASE ACTIVITY OF DDVP-TREATED PALM WEEVIL (RYNCHOPHORUS PHOENICIS) LARVA BY ADEWUMI‚ SEGUN JOHN BCH/07/1313 IN PARTIAL FULFILMENT FOR THE AWARD OF BACHELORS IN TECHNOLOGY (B. TECH.) IN APPLIED BIOCHEMISTRY IN THE DEPARTMENT OF BIOCHEMSISTRY‚ FEDERAL UNIVERISTY OF TECHNOLOGY‚ AKURE‚ ONDO STATE OCTOBER‚ 2012 CERTIFICATION This is to certify that this project work was carried out by Adewumi Segun John‚ with matriculation number BCH/07/1313‚ student of the Department
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