EFFECT OF pH LEVEL ON THE ENZYME ACTIVITY OF INVERTASE John Dale Cruz‚ Michael Ray Dela Cruz‚ Karen Angelica Domalaon‚ Abegail Dulay‚ Shirley Ann Felipe Group 5 2C Medical Technology Biochemistry Laboratory ABSTRACT Invertase is a type of enzyme‚ a natural catalytic agent for biochemical reactions‚ can be obtained in Baker’s Yeast. Determination of the effect of pH on invertase activity is the primary objective of the experiment. Dinitrosalicyclic acid (DNS) Assay method is utilized to monitor
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12 Appendix 13 References 13 Journal 14 1 Design 1.1 Defining the problem Research Question This lab will be driven by the research question; do changes in temperature (from 0˚C‚ 7˚C‚ 21˚C‚ 37˚C‚ and 90˚C) have an effect on the activity of the enzyme catalase (found in beef liver) in the breakdown of hydrogen peroxide? Hypothesis If liver is placed in different temperatures of 0˚C‚ 7˚C‚ 21˚C‚ 37˚C‚ and 90˚C and hydrogen peroxide is added to each piece of liver‚ then the liver placed
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Research Question: Effects of pH on amylase activity Introduction: Amylase is an enzyme that is in human’s saliva as well as the pancreas. Enzymes are biological catalysts that speed up a chemical reaction. They break down complex molecules into simple ones. In this case‚ amylase converts starches (complex molecule) into simple sugars. That is why foods like potatoes for example‚ may taste sweet to us‚ because they contain starch. The optimum pH for pancreatic amylase is the pH of 7. In the
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Effect of pH on Enzyme Activity Athenaa Jeyachandran Mr.Menes Course Code January 21st‚ 2013 The Effect on Enzyme Activity Due to Temperatures Purpose: The purpose of this lab experiment is to examine how different temperatures affect enzyme activity using hydrogen peroxide. Hypothesis: My hypothesis for this experiment is that temperatures near body temperature is when enzyme activity will be at its highest. I believe this will occur because in our body‚ enzymes
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How does pH affect the activity of the Amylase? Abstract: In my experiment I aimed to observe how ranging pH levels will affect the rate in which amylase will break down the starch molecules. I will be measuring the time it takes for the dark liquid to disappear and leave a yellow brown liquid to be shown‚ which would show that there is no starch present in the solution because it would have broken into maltose by adding amylase. Results did not fully demonstrate what we expected in our hypothesis
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IB Biology Sheran Gu Meghan Lena Nov. 15th Influence of pH on Enzyme Activities How does the pH of solution influence the speed of enzyme activities? Enzymes are protein catalysts and chemical that speed up chemical reaction without being involved in the reaction. They are involved in the human digestive system to help people digest food more efficient. Food are substrates to be break down and absorb through the digestive system in the body. Substrate is a molecule that bond with the active site
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DNA Structure Monday‚ 15 April 2013 9:01 AM - DNA = Deoxyribonucleic acid DNA is a double-helix: it has two strands that twist around each other Each strand is made of single units call nucleotides It has a sugar-phosphate backbone Bases join the two strands by hydrogen bonds ○ These bases are cytosine‚ guanine‚ adenine and thymine. - Complementary base pairing is a key idea in genetics: C pairs with G‚ and T pairs with A. - Each strand of DNA can be millions of base pairs in length and is
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Investigation into how pH levels affect Catalase activity in potato samples – (enzymes) Introduction: The aim of this investigation is to understand and monitor the effects pH levels have on catalase activity within controlled potato samples. Catalase is a type of enzyme that is found widespread among organisms that grow in the presence of oxygen (almost all living things) its specific use is to speed up the decomposition of Hydrogen peroxide (H2O2) within cells. Hydrogen peroxide is a bi-product
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Testing Catalase Activity Prepared by Anel Behric For professor Goodrow General Biology Lab Mohawk Valley Community College 10/14/2014 6:00 PM Introduction: This experiment was conducted to examine the breakdown of substrate hydrogen peroxide (H2O2) by catalase‚ which is a specific enzyme that breaks down substrates of (H2O2). Molecules are in our bodies and nature. They move around constantly which causes them to be part of random collisions‚
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Research Question: How will an increase of FeSO4 concentration affect the activity of the enzyme catalase? Hypothesis: By increasing the concentration of FeSO4‚ there will be a decrease in the activity of the enzyme catalase because the Iron (ii) in the metallic ion will act as an inhibitor during the catalysis of the hydrogen peroxide into O2 and H2O. Introduction: We rely on oxygen to provide us with energy (ATP) and go through all the phases of aerobic respiration. However‚ oxygen can be
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