The Effects of varying p-Nitrophenyl Phosphate concentrations on Alkaline Phosphatase Activity Nathan Overholt Louis Settembrino March 5‚ 2002 Abstract: The enzyme Alkaline Phosphatase was set up in solutions of varying p-nitrophenyl phosphate concentrations. A fixed time assay was used to find the Michaelis constant and Vmax. All solutions were incubated at 30 C° for 30 mins. Absorbances were measured at 405 nm‚ and the Km was found to be 0.002 M (pNPP) and the Vmax to be 0.433 A/min. Introduction:
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determining the effects of pH on enzyme activity show that as the independent variable‚ pH‚ increases the dependent variable‚ percentage transmission‚ decreases. This is shown in the results as at the lowest pH‚ pH 4.0 the average percentage transmission is at its highest at 97%. At the highest pH‚ pH 8.0 the average transmission is 78.5%. This is also supported from the graph as it produces a negative gradient showing that as the percentage transmission will decrease with an increasing pH. This happens
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Enzyme Catalase Activity in Reaction with the Substrate Hydrogen Peroxide Abstract We performed these experiments to observe the effects of enzymes on the rate of reactions. We tested and compared the activity of the enzyme catalase on the substrate H2O2 in various states and percentages‚ and observed the absorption values of the enzyme-substrate relationship at different concentrations. Our results show that the more substrate available‚ the quicker the reaction will happen except in one test
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The Affects of pH on Peroxidase Enzyme Claire Bell Biology 1130 Jazmen Myers Section 36 Abstract Peroxidase is a turnip enzyme; it is used in the oxidation of hydrogen peroxide to lower activation energy‚ speeding up the reaction. The activity of peroxidase is highly dependent on its environment and most importantly the pH level. Peroxidase has been the focus of many recent studies and is believed to possibly reduce swelling among other things. We conducted an experiment testing
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only as an annex named‚ Marikina High School Annex. By the year 1993‚ Mrs. Lagrimas B. Garcia‚ the school principal‚ made extra effort on achieving full independence of the Marikina High School Annex to give birth to the school‚ Parang High School (PHS). PHS had been through a lot of changes since the School Year 1996-1997. Hon. Congressman Romeo DC Candazo‚ Hon. Bayani BH Fernando and Dr. Perla B. Menguito made a big impact on the transformation of the school’s image and into a conductive learning environment
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Name: _____________________ Date: _____________ Student Exploration: pH Analysis Vocabulary: acid‚ acidic‚ alkaline‚ base‚ indicator‚ neutral‚ pH Prior Knowledge Questions (Do these BEFORE using the Gizmo.) 1. Acids are substances that produce hydrogen ions (H+) when dissolved in water. Lemon juice is an example of an acid. A. What does lemon juice taste like? __Sour.______________________________ B. What does it feel like if lemon juice gets in your eye? ___It
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Awards/Honors‚ Health Care Exposure‚ Community Service‚ Research‚ Leadership‚ Extracurricular Activity‚ Employment‚ and Other. In the "Description of Role" section of each activity‚ please provide additional details regarding how it correlates with any other "Activity Type." The admissions committee takes into account the fact that some activities may fit multiple categories. Activity Type: Health Care Exposure Activity Name: Pharmacy Technician Organization Name: CVS pharmacy Contact/Supervisor Name: Dina
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Pre-primary and Year 1 This resource contains information about what children need to learn and be able to apply as they read‚ write and spell. It outlines what teachers can do to assist children to develop phonological awareness and phonic knowledge. Activities are suggested to further assist teachers with ideas for teaching the content outlined. http://ecm.det.wa.edu.au/connect/resolver/view/PDLP1001/1.1/PDLP1001.pdf Year 2–3 grammar and punctuation This resource contains information about what children
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ID:0720052 Effect of an Increasing Substrate Concentration on Enzyme Activity Rate Abstract The reaction rate of an enzyme can be affected by many factors‚ and the purpose of this experiment was to find out how an increasing substrate concentration influences the rate of an enzyme activity; we obtained data from recording the absorbance of the samples which contain the same amount of potato juice (enzyme oxidase) and different amount of catechol (substrate) while holding pH and temperature
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bacteria. For Unknown A‚ I ran six tests. I first isolated the bacterium from the second bacterium and found a clear growth (Table 1‚ Figure 1). Secondly‚ I ran a gram stain and found a gram positive‚ cocci bacterium (Table 1‚ Figure 2). Third‚ I ran a catalase test in which was negative (Table 1). From here‚ I determined a starch hydrolysis test would be necessary to distinguish between different bacteria. The result was negative (Table 1). After starch hydrolysis‚ I ran an MSA plate‚ hoping it would give
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