An Introduction to DNA microarrays Rebecca Fry‚ Ph.D. http://www.buffalo.edu/UBT/UBT What is a DNA Microarray? genes or gene fragments attached to a substrate (glass) Tens of thousands of spots Hybridized slide Two dyes Image analyzed 1 The Beginnings of Microarray Technology Lockhart et al.‚ 1996 Nature Biotechnology “Expression monitoring by hybridisation to high-density oligonucleotide arrays” Schena et al.‚ 1995 Science “Quantitative monitoring of gene expression
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Report August 28‚ 2013 DNA Extraction on Onion (Allium cepa) and Human Cheek Cell Arriza‚ Rolland Merch Buscato‚ Carl G. Butil‚ Conrad G. Leonida‚ Renee Theresa ABSTRACT This activity observes the DNA present in onion (Allium cepa) and human cheek cells by extracting it with the addition of lysis buffer and chilled ethanol. The lysis buffer is prepared from squeezed onion mixed with salt and Pantene Pro-V Shampoo. The buffer degrades the protein enveloping the DNA found in onion and cheek
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DNA Sequencing As of last few weeks‚ the transformation lab is performed to convey and purify a given protein. However after further research scientists found out that Transformation is not only used to purify protein but also to find out contents that are stored in a given plasmid. The objective of the lab that is to be performed involves a procedure that determines the identity of an unknown gene replicated in a plasmid. To begin this procedure two to four colonies of bacteria is added to two
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2014/15 Module name and number Molecular Biology & Genetic Engineering: BS941 Assignment title “DNA sequencing: where we are and where it’s going” Student Number 1464986 Word Count 2310 The article focuses on the advances achieved in DNA sequencing by first providing a brief background on DNA‚ and how it was initially sequenced. The paper then takes into consideration four of the major DNA sequencing techniques. These include: Sanger’s Chain Termination Method‚ Pyrosequencing‚ Single Molecule
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DNA and Evidence DNA is one of the most important roles to evidence and in a criminal case. It helps to prove a convict guilty or help those wrongly accused or convicted. DNA stands for deoxyribonucleic acid. Just about every cell contains DNA. The DNA that’s in people blood is also the same DNA in people’s hair‚ bone‚ saliva‚ skin‚ tissue and everything else. What’s great about someone’s DNA it does not ever change throughout their life. DNA was first used as a way of finding out paternity so
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DNA Worksheet Answer the following in at least 100 words: 1. Describe the structure of DNA. A molecule of DNA is made up of long chains of polymers and monomers called nucleotides. Those chains‚ two in particular that compose a strain of DNA‚ are formed by the grouping of nucleotides into polynucleotides. A nitrogenous base‚ a sugar‚ and a phosphate group make up the composition of a nucleotide. In the case of DNA‚ the four nucleotides that are found along the chain of DNA are thymine (T)
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Draft 5\9\10 Understanding DNA Have you ever wondered how you got red hair or blue eyes? Well all that has to do with your genes. To have different genes you have to have a deoxyriboncleic acid or DNA for short. Without Dna everyone would and everything would look the same and that would make life really confusing. Your DNA has a very important role in life. Its most important role is to give everyone character. Every Dna chromosome is made the same according to species
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DNA Cloning ~ The Future of Science DNA Cloning DNA cloning is the process where an exact replica of an organism is produced. Some scientists have already produced clones of animals; Dolly the sheep is one of the many examples of DNA cloning. Cloning is a natural process‚ many living things only need one parent to reproduce‚ this process is called asexual reproduction; for example single-celled bacterium use this process. The new bacteria inherits its genes from only one parent‚ this means
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The amazing advances in health science‚ DNA is transforming the way in which criminal investigations and trials are carried out. DNA evidence and its importance can rest on a single fact: Every individual’s DNA is unique. A person’s DNA profile can be used similar to a fingerprint to link suspects to crime scenes and its victims. DNA profiling—which is also called DNA fingerprinting or even DNA typing—has been responsible for overturning verdicts and saving innocent people from execution. The process
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An example of modern forensics evidence is the use of DNA fingerprints. Sources of DNA include blood‚ hair‚ semen‚ saliva‚ bone and tissue. Each person has a unique DNA fingerprint. A DNA fingerprint is the same for every cell‚ tissue‚ and organ of a person. It cannot be altered by any known treatment. Consequently‚ DNA fingerprinting is rapidly becoming the primary method for identifying and distinguishing among individual human beings . An additional application of DNA fingerprint technology is the diagnosis of inherited disorders in adults
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