Affect of enzyme concentration to the rate of reaction Aim: With the experiment of protein solution‚ in this case egg white added to different pepsin concentrations (0%‚ 0.2%‚ 0.4%‚ 0.6%‚ 0.8%‚ 1.0%) shows‚ as the egg white is a protein and the pepsin works as an enzyme‚ how a higher pepsin concentration and therefore a larger amount of enzymes effect the rate of reaction. Hypothesis: An increased concentration of pepsin speeds up the time the mixture needs to come clear. Introduction:
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The Effects on Enzymes By Bailey Rose The Effects on Enzymes Bailey Rose 10/31/2011 Abstract In this lab exercise‚ the study of enzyme catalase‚ we viewed the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase from starch and measure the rate of activity under different conditions. Changes in temperature and pH along with Substrate Concentration and Enzyme Concentration were the conditions tested in the experiment. Our class performed
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HINTS ON WRITING YOUR REPORT Title: Enzyme activity Objective: To investigate the effect of temperature on amylase activity Design principle Background: Amylase activity products? (show the equation) Which factors will affect enzyme activity? How to study the rate of reaction? (e.g. rate of disappearance of substrates or rate of formation of products) Independent variable: temperature of reaction mixture or at which the enzymatic reaction occurs. It can be varied by setting water bath at
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Effects of Temperature on Enzyme Biology Introduction In order to understand the activity of enzymes at different temperatures the ability of the enzyme to function can be measured. This is important in many applications such as Polymerase Chain Reaction for forensics as well as genetics research where manipulation of temperature-dependent enzymes allows for replication of DNA segments. Bennett states‚ “when the energy - measured
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Aim: To investigate the effect of hydrogen peroxide concentration on enzyme catalase Indroduction: Enzymes are proteins. They function as biological catalysts. They lower the energy barrier of a reaction so that the reaction can take place at body temperature. Also‚ they can speed up Metabolic reactions without being changed or used up. During a reaction‚ an enzyme molecule combines temporarily with the substrate. When the reaction is complete‚ the enzyme molecules returns to its original dorm
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between catalase concentration and the rate of reaction. This means when catalase concentration increases‚ the rate of reaction also increases. In other words‚ at 20% catalase concentration‚ the rate of reaction was only 4.220 mm/s while at 100% catalase concentration‚ the rate of reaction was 7.704 mm/s. This proves the positive correlation between catalase concentration and the rate of reaction. This occurs because as the enzyme concentration increases‚ there are more enzymes available to catalyze
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Structure: Enzymes are globular proteins that act as catalysts‚ they have a specific 3D shape that is the result of their amino acid sequence. There is a specific region of the enzyme that is functional‚ this is called the active site. The active site is made up of a small number of amino acids and forms a small depression within the larger enzyme molecule. Moreover‚ the molecule that the enzyme acts upon (substrate) fits precisely into the depression and forms an enzyme-substrate complex. The substrate
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Internal assessment on enzyme concentration Enzymes are proteins that act as catalysts and they are the most important type of protein. My aim in this experiment was to investigate the effect of enzyme concentration on the rate of an enzyme (protease) – catalyzed reaction ‚ catalysts speed up chemical reactions . My hypothesis is that the rate as reaction will increase as the concentration of protease in the solution increases so it will take less time
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Cellular respiration is defined as an enzyme mediated process in which organic compounds such as glucose is broken down into simpler products with the release of energy (Duka‚ Diaz and Villa‚ 2009). It is a series of metabolic processes and oxidation-reduction reactions. Oxidation of substrates‚ such as glucose‚ is a fundamental part of cellular respiration (Mader‚ 2009). As a catabolic process‚ it may or may not require the presence of oxygen. The process that requires oxygen is called aerobic
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College General Biology I Enzymes‚ test for effect of pH on catalase activity Purpose The main purpose of this experiment was to learn about enzymes and how to test for the effect of pH on catalase activity and to be able to tell if a reaction is an exergonic or endergonic process. Introduction Enzymes are made from amino acids‚ which are made from proteins. In order to make an enzyme‚ hundreds of amino acids are strung together in a very specific and unique order and eventually is folded
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