Lab #6: pH Effects and Enzyme Concentration on Catecholase Activity Abstract: This experiment examined the effect on how the changes in pH balance of potato juice affected the enzyme activity‚ structural stability and solubility. Introduction: Enzymes are macromolecules that allow chemical reactions to occur. They function as biological catalysts. A catalyst is a substance that is involved in‚ but not changed by or consumed in‚ a chemical reaction. The amount of energy required for a reaction
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the enzyme catecholase in different pH levels as well as its absorbance in differently concentrated solutions. A spetrophotometer was used to measure the absorbance of the enzyme catecholase in different pH solutions as well as to measure the absorbance of catecholase in solutions with different concentrations of potato juice and phosphate buffers. Absorbance of the enzyme catecholase was at an optimum level when pH was close to neutral. When pH was acidic or basic‚ the catecholase was less effective
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of this study is to measure the effects of external factors of temperature‚ pH and enzyme concentration levels and how it effects the catalyst reaction times of catecholase and peroxidase enzymes using potato extract. A review on how enzymes react under certain external conditions gave a good foundation to base this experiments hypothesis when looking at concentration of enzymes‚ temperature and pH levels.
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Investigating the Enzymatic Activity of Catecholase through Temperature‚ pH‚ Enzyme Concentration‚ and Substrate Concentration University of Alabama at Birmingham Burgess‚ B.N. Introduction: Background Enzymes are macromolecules that act as catalysts in living organisms by speeding up chemical reactions without being changed or destroyed by the reaction (Campbell and Reece‚ 2008). Enzymes are able to speed up the rate of a chemical reaction by decreasing the activation energy during the reaction
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December 2012 1. Title and Author 1. The Effect of Extreme Temperatures on the Rate of Photosynthesis 2. Jeffrey Xia 2. Abstract A previous lab in which we conducted‚ tested whether or not the light intensity had an effect on the rate of photosynthesis. We concluded that light intensity did possess an effect on the rate of photosynthesis – the closer the experimental plant units were to the light source‚ the more experimental plant units exhibited the effect of gas exchange in photosynthesis.
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process can be affected by several variables; one of the most notable variables is the temperature of the plant’s environment. Previous study has led me to conclude that when the temperature of the plants’ environment is low‚ the rate of photosynthesis is also low because the enzymes responsible for photosynthesis are not able to absorb as much heat energy and in turn will move slower. In contrast‚ if the temperature of the plant’s environment increases‚ the rate of photosynthesis will also increase
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Lab Report The effect of temperature on the reaction between Catalase and H2O2 Sarah AlShemesi In this experiment we’ll be exploring the effects of temperature on the reaction between Catalase and H2O2.We’ll be using five different temperatures to test this. The five different temperatures will be 10‚ 30‚ 50‚ 70 and 90 oC. We will use the liver as a source of Catalase. A 1 gram piece of liver will be inserted into a test tube with 2 cm3 of water‚ then 2 cm3 of H2O2 will be added. The Catalase
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first lesson week1‚ term 1‚ 2013Risk Ass Due: last Lesson week 2‚ Term 1‚ 2013Final: First lesson week 6‚ Term 1‚ 2013Procedure: Your task is to investigate the effect of temperature on the activity of enzyme catalase found in beef liver. Use the experimental design and the BioLab Guide provided to assist with your report write up. Safety Considerations: You must have the risk assessment checked before continuing. NOTE:
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The Effect of Temperature on Solubility By Aviraj Singh Rogers 2 Background: The solubility of most solid substances is generally said to increase as the temperature of the solvent increases. However‚ some substances‚ such as ytterbium sulfate‚ do the opposite. This can be explained through the Second Law of Thermodynamics which states that “in all energy exchanges‚ if no energy enters or leaves the system‚ the potential energy of the state will always be less than that of the initial state”
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WHY DOES THE COLOUR LEAK OUT OF COOKED BEETROOT? PLAN Hypothesis Temperature has an effect on the leaking of the colour from beetroot. This means that as the temperature is altered there will be a change in the rate of colour leakage. Scientific Background Beetroot is a very familiar vegetable and is commonly known as beet. It is famous in most recipe books that advice that its outer skin is not to be removed to avoid getting red dye in the cooking water. If we look at the internal structure
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