Effects of Temperature‚ pH‚ Enzyme Concentration‚ and Substrate Concentration on Enzymatic Activity INTRODUCTION Enzymes‚ proteins that act as catalysts‚ are the most important type of protein[1]. Catalysts speed up chemical reactions and can go without being used up or changed [3] Without enzymes‚ the biochemical reactions that take place will react too slowly to keep up with the metabolic needs and the life functions of organisms. Catecholase is a reaction between oxygen and catechol
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Effect of Catalase at Different Temperatures Abstract The role of this experiment is to determine the effect of temperature on enzymatic activity. The results of the experiment were the colder the temperature the slower the reaction rate and the hotter the temperature the faster the reaction rate. Introduction Enzymes are chemical substances found in living cells and they act as catalysts of the various chemical reactions that occur in them.(Preszler‚ 2012) They bind to substrates that have
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Temperature effects on the growth of microorganisms Introduction: The purpose of this experiment was to determine the effects that temperature has on three different organisms. Temperature is one of the most important environmental factors affecting growth and survival of microorganisms². The three organisms used where Escherichia coli‚ Pseudomonas fluorescens‚ and Bacillus stearothermophilis. Most bacteria grow within a particular temperature range. The minimum growth temperature is
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phosphorylase in particular) are affected by varying its temperature before introducing it to the substrate it will be reacting with. A catalyst (enzyme) is a substance that changes the rate of a reaction; for a reaction to take place at all‚ the enzyme must first come into contact with the substrate. Enzymes are subject to a number of factors which effect how fast they can cause a reaction with a substrate; these factors include temperature‚ pH levels‚ chemical substances and the concentration of
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Investigating the effect of temperature on plant cell membranes The purpose of this activity is: • to practise experimental and investigative skills • to investigate the effect of temperature on cell membrane structures Procedure SAFETY: Always carry scalpels clasped to a tile and with the tip pointing away from you. Beetroot cells contain pigments called betalains that give the tissue its dark purple-red colour. The pigment is contained in the cell vacuole. Investigation
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PROCEDURE: Part A (Effect of temperature on growth) 1) 15 tubes of glucose broth are provided and one set of 3 tubes are inoculated with each of the following cultures; Escherichia coli‚ Pseudomonas fluorescens‚ Micrococcus luteus and Saccharomyces cerevisiae. The last served as control. 2) One of the three tube of each culture is incubated at each of the following temperature: * 4°C * 37°C * 55°C 3) All the tubes are incubated within 5 minutes after inoculating. The turbidity
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aim of this experiment was to determine the effect of caffeine on resting body temperature. This has to do with homeostasis‚ which is the maintenance of equilibrium. In other words‚ it is a stable body state. Homeostasis refers to the process of keeping the internal body environment in a steady state‚ when the external environment is changed (Homeostasis 2). Humans are warm-blooded creatures that generate body heat internally and maintain body temperatures at a fairly constant level – about 98.6oF
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Title: “The Effect of Substrate Concentration‚ Enzyme Concentration‚ pH and Temperature on Enzyme Activity” Abstract: In the following experiments we will measure precise amounts of potato extract as well as Phenylthiourea‚ combined with or without deionized water and in some instances change the temperature and observe and record the reaction. We will also investigate the different levels of prepared pH on varying samples of the potato extract and the Phenylthiourea and record the results.
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investigate the effect of temperature on the enzyme catalase. The original research question was exploring the effect temperature would have on a yeast catalase reacting with hydrogen peroxide (H2O2). To address the latter question a series of experiments were conducted. The various temperatures experimented with were as follows: 22 degrees Celsius (room temperature)‚ 0 degrees Celsius (freezing)‚ 100 degrees Celsius (boiling)‚ and 37 degrees Celsius. Along with variations in temperature‚ the experiment
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The Effect of Temperature on Blow-fly Larvae INTRODUCTION Calliphoridae- comonly known as a blowfly‚ are living organisms which usually are the first insects to colonise a body after death. The blowfly larvae are very useful in forensic entomology. The oldest larvae give an approximate time of death of body. Family: Calliphoridae Order: Diptera Name: Calliphora vicina Aim: To check the influence of temperature of environment on living functions and activity of Blow-fly larvae.
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