Temperature effects on the growth of microorganisms Introduction: The purpose of this experiment was to determine the effects that temperature has on three different organisms. Temperature is one of the most important environmental factors affecting growth and survival of microorganisms². The three organisms used where Escherichia coli‚ Pseudomonas fluorescens‚ and Bacillus stearothermophilis. Most bacteria grow within a particular temperature range. The minimum growth temperature is
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Diffusion Introduction Diffusion is the net movement of ions or molecules from a region of high concentration to a region of low along the concentration gradient until equilibrium has been reached. The steeper the gradient the faster the rate of diffusion. There are a few types of diffusion. Three (3) of those are: Facilitated Diffusion- refers to diffusion of substances across a cell membrane with the help of transport protein. Dialysis- refers to the diffusion of solutes across a semipermeable
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Effect of temperature on enzyme activity In this experiment… Independent variable: Temperature of the amylase Dependent variable: Enzyme activity which is measured by the time for disappearance of starch Controlled variables: Volume of amylase; volume of starch solution; concentration of amylase; concentration of starch solution Prediction of results i) At low temperature‚ the rate of amylase activity is very low. ii) At optimum (=best) temperature‚ the rate of amylase activity is the
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Lab Report Effect of temperature on enzyme activity I. Purpose: Determine how effect of temperature on enzyme (catalyse) activity (in the liver). II. Materials * Raw liver * Forceps * 50mL 1% hydrogen Peroxide (H * 25mL graduated cylinder * 50mL‚ 400mL beakers * Pureed liver * 5 Filter-paper disks * Paper towels * Timer or stopwatch * Thermometer * Hot plate III. Procedure 1.Gather all necessary materials; start
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Experiment 4 – Effect of Temperature on Enzyme Activity Aim To study the effects of temperature on the activity of amylase enzyme on starch solution. Introduction Enzymes are widely known as biological catalyst. Almost all cellular reactions are controlled and guarded by enzymes. Virtually every metabolic reaction which takes place within a living organisms are catalyzed by enzymes. Enzymes are complex three-dimensional globular proteins. Some of the enzymes are built up off proteins and
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The Effect of Molecular Weight and Time on the Diffusion Rate of Potassium Permanganate‚ Potassium Dichromate‚ and Methylene Blue.1 Arantxa Alex Carpio Group 1 Sec. X – 4L March 24‚ 2015 ABSTRACT The effect of molecular weight and time on the rate of diffusion was determined using the agar-water gel test. A petri dish of agar-water gel with three wells was prepared and a prepared solution of each substance was dropped on each well; one with potassium permanganate (KMnO4)‚
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test for the effects of temperature on catalase activity. Introduction: Enzymes are proteins that act as catalysts and help complex reactions to occur everywhere in life. Enzymes affect a chemical reaction by acting as a catalyst‚ which is to say‚ it speeds up the reaction. However‚ extreme ph and temperature hinders enzyme function. Optimal activity of an enzyme is the optimal environment that an enzyme requires to function‚ which means having the optimum ph and temperature to catalyze a
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aim of this experiment was to determine the effect of caffeine on resting body temperature. This has to do with homeostasis‚ which is the maintenance of equilibrium. In other words‚ it is a stable body state. Homeostasis refers to the process of keeping the internal body environment in a steady state‚ when the external environment is changed (Homeostasis 2). Humans are warm-blooded creatures that generate body heat internally and maintain body temperatures at a fairly constant level – about 98.6oF
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Investigating the Effect of Temperature on Enzyme Activity Almost all chemical reactions that occur in living organisms are catalyzed by enzymes. Many factors in a cell’s environment affect the action of an enzyme. In this investigation‚ you will design an experiment to determine the effect of temperature on an enzyme-catalyzed reaction. You will complete an entire lab write-up using the CHS LAB REPORT FORMAT. Fundamental Question How does temperature affect the rate of an enzyme-catalyzed
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Effect of different temperatures on the rate of an enzyme controlled reaction I will place starch and amylase into five water baths which are at different temperatures‚ and record the time it takes to break down the starch in the solution. Independent variables The independent variable is what I am going to change in my experiment. In this case it is the temperature of the water in the five water baths- 10‚ 25‚ 40‚ 55‚ 70 degrees Celsius Dependant variable This is what will stay the
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