The purpose of this experiment is to separate a solution of an acidic and organic compound and purify its respective acidic product through the techniques of an acid base extraction‚ recrystallization‚ and use of Melt Temp (melting point range). The reaction of an unknown acid (3-chlorobenzoic acid) with diethyl and sodium hydroxide was observed‚ and the solution naturally separated into an aqueous and organic solution. The mixture of acid and organic solution was created and then separated through
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SOP: Solid phase extraction of cylindrospermopsin in filtered water samples Document identifier: SOP_TOXIC_UDU_05F Prepared by: James S. Metcalf and Geoffrey A. Codd‚ UDU Date: 7 July 2005 1 Introduction In order to determine the concentration of cylindrospermopsin in the extracellular fraction of filtered environmental waters‚ solid phase extraction (SPE) is necessary to concentrate the toxin to concentrations capable of being detected by HPLC. 2 Experimental 2.1 Materials
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called extraction. Extraction is a systematic process of separating mixtures of compounds‚ taking advantage of the affinity differences of compounds to separate them (Padias 128-37). This technique recognizes the principle that “like dissolves in like‚” that is‚ polar solutes dissolve in polar solvents and non-polar solutes dissolve in non-polar solvents. Through extension of this principle‚ one can use extraction to separate compounds of a mixture. There are three different methods of extraction; solid-liquid
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Berry DNA Extraction Lab I. Problem Statement: Successfully extracting the DNA from strawberries. II. Background info: DNA is the genetic information in organisms that codes for proteins‚ and controls the cells function. The DNA is a double helix molecule that is formed by various nucleotides which include Adenine‚ Thymine‚ Guanine‚ and Cytosine with sugar phosphate. Different organisms can have larger or smaller genes. DNA is also what makes up chromosomes during cell replication. Strawberries
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Introduction Extraction is a purification technique used in organic chemistry to separate compounds from a mixture of two or more compounds. There are three different extraction techniques: liquid-liquid extraction‚ solid-liquid extraction and chemically active extraction. All three types of extraction follow the same principle. Organic molecules dissolve in organic solvents and polar molecules dissolve in aqueous solvents. This phenomenon is observed because of the intermolecular forces between
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Tan 1 DNA EXTRACTION Aim : To extract the DNA from an egg yolk using various enzymes and to compare with other groups the most effective way to extract DNA. Hypothesis : To be able to observe white springy substances after mixing with enzyme and alcohol. Apparatus : -Test tube‚ spatula‚ glass rod‚ dropper‚ beaker‚ test tube rack‚ skewer. Materials : - 1 egg‚ meat tenderizer‚ salt‚ water ‚ soap‚ isopropyl alcohol 91%‚ pineapple juice. Variables : Manipulated Variable : Responding
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Egg Donation and Overcompensation Imagine you’re a twenty-two year old female‚ fresh out of college. You have a big shiny college diploma from a highly prestigious University hanging in your bedroom‚ yet are still interning for no pay and looking for your first “big-girl” job. Your apartment‚ electricity‚ and cable bills didn’t end when you stopped receiving financial aid‚ and now you are required to start paying off those loans. You are looking for quick money and spot an ad online looking for
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its mixture using acid-alkaline extraction. 2. To determine the percentage recovery and melting point of benzoic acid and p-diclorobenzene. Apparatus:Separatory funnel(250mL)‚Buchner funnel‚beaker. Materials:Benzoic acid‚p-dichlorobenzene‚ether‚10% NaOH‚conc.HCl‚distilled water‚ anhydrous CaCl2. Introduction: Organic compounds in an aqueous mixture can be separated by shaking the aqueous solution with a solvent which is immiscible with water. Extraction involves dissolving either a
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’ is the embryo‚ which is the part of the seed that can grow into a new wheat plant. (1) Aim: To extract DNA from Wheat Germ cells‚ and learning the DNA extraction process. Hypothesis: Each step of the process will help in a certain way to extract DNA‚ until we are finally successful in the end. In the wheat germ extraction‚ the ethanol will be able to separate the DNA‚ and it will float between the less dense ethanol and the denser homogenizing mixture. (2) Materials: 1 gram
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Bylaws by Laws (Laws that are often Violated by Fraternity Initiations) I. Introduction Thesis statement: Fraternities are bad. Background: The recent news about hazing deaths‚ cause mass uproar from parents that want their children to go to school without fear of their children dying. II. Body: 1. Criminally Negligent Manslaughter 2. Universal Declaration of Human Rights: Article 5 3. Republic Act No. 8049 III. Conclusion:
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