Extraction and Analysis of Plasmid DNA from E. coli Cells Introduction A plasmid is an extra-chromosomal element‚ often a circular DNA. Since a plasmid is by definition an extra-chromosomal element‚ it cannot make use of any origin of DNA replication in a chromosome (BP site). Meaning that DNA synthesis within a plasmid depends on having an origin of DNA synthesis of its own. Plasmids are often found in bacterial cells‚ in which they are used as transfer agents for transmitting various antibiotic
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“S-Pure” the premium and super hygienic fresh eggs Betagro groups are the company groups produce high quality of agriculture‚ meat processing and food product under concept “Let’s make life better”. Their main markets are mostly export to Japan and Europe. “S-Pure” local premium brand is one of product line to positioning fresh pork and chicken product with healthy from high quality of raw material‚ close system and antibiotic free and e-traceability and super hygienic of food safety & quality
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roof‚ and four sliding windows used for a mud pie restaurant drive-through. “Let’s play Colored Eggs!” Ethan said. “All right.” The rest of us excitedly agreed. “I get to be the wolf!” Ethan called running to the opposite corner of the yard. There he waited for us to call “ready”. Strolling into the playhouse we decided Rebecca was a green egg‚ Ashlyn was a pink egg‚ and I was a blue egg. “Ready” we called. Racing to the tightly shut playhouse door Ethan pounded three times. “Who
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My favorite Dr. Seuss book is “Green Eggs and Ham.” I picked this book because my mom read it to me all the time when I was little boy. Whenever she would read it to me it would always put a smile on my face. My favorite part of the book was when the “Who” that doesn’t like the green eggs and ham finally agrees to try them and likes them‚ “Say! I like green eggs and ham! I do! I like them‚ Sam-I-am!” (59) Dr. Seuss’ book “Green Eggs and Ham” is the story of a who named Sam-I-am that encourages
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symbolism from the the East and West Eggs‚ colors‚ class systems and even the meaning of money. The East and West Eggs Hold much more than homes and people‚ they hold secrets and truths. In Scott Fitzgerald’s The Great Gatsby the East and West Eggs are not as they appear; the root of all character conflict goes back to these two homes. The Great Gatsby opens with our narrator‚ Nick Carraway‚ is visiting his cousin Daisy Buchanan in New York. Nick moves to the East Egg and becomes the neighbor of Jay
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Jay Gatsby‚ and his inability to fulfill his dreams. Even in the first chapter of the novel‚ the pessimism focused on the American Dream rings clear through F. Scott Fitzgerald’s words. The story is set in the New York City area‚ including West Egg‚ East Egg‚ and the Valley of Ashes. These locations are symbols
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Making owls from egg shells Get wise to these birds made from egg shells. Pencil holder from eggshell You need: translucent plastic egg carton‚ CD‚ toilet roll and torn strips of newspaper and magazine pages Eggshell 2 translucent plastic egg-carton cups Toilet roll Newspaper strips Magazine pages CD Scrap paper – 2.5cm x 4cm PVA and UHU glue Pencil Black marker Acrylic paint Brush Scissors Piercing tool Making the owl pencil-holder 1. Gently tap a small hole on the
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four of The Great Gatsby‚ “green rakes the recognizable form of money‚ or at least the things money can buy” (Kersh). Gatsby is well known for his extravagant parties and huge mansions. Gatsby is from the west egg‚ which symbolizes new money. The West Egg is the “less fashionable of the eggs” (Abuzahra). Gatsby’s house reflects him as a person‚ showing how much of a lonely person he is. He has a huge house and he only lives there. Gatsby throws huge extravagant parties but does not interact with most
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Experiment 2 Titles Extraction of Bacterial Plasmid DNA and Analysis of Extracted DNA Samples Objectives * To learn the procedures needed in extracting the bacterial plasmid DNA * To determine the concentration of original DNA sample and purity of prepared DNA sample by using spectrophotometer * To analyze the extracted DNA sample by gel electrophoresis Materials and methods (Refer to UDBB2144 Laboratory 2A Manual Principles of biotechnology page 6-10) Results
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character’s lives however the original American dream is replaced by a degraded dream greed and wealth. In The Great Gatsby we encounter an extraordinary individual‚ Gatsby‚ whose immense wealth catches the eye of many speculators in New York‚ West Egg and East Egg. In the story the author makes sure to emphasize the decay of the original American dream. The American dream originally represented hope and equality‚ for everyone looking for a better life. However after the wars and the passing years people
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