inhibitors of growth. Today‚ there are are number of well established techniques commercially available as kits which make DNA extraction and purification easy. Although kits offer fast and reliable methods for extracting plasmid DNA with high yields‚ kits are expensive. Since a PCR is performed afterwards‚ which amplifies certain DNA fragments‚ we use miniaturized extraction methods; the extracted amount is large enough for further processing. PCR‚ a method to amplify DNA: For details see lectures
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Chemical Aspects of Life and Spit Lab Formal Report 1. Abstract The objective of the Reducing Sugar Test was to test if the substance has a reducing sugar in it by adding Benedict’s solution and heating it‚ there would be a color change if a reducing sugar is present‚ or it will remain blue (no reducing sugar). The objective of the Starch test was to test for starch in substances by using Iodine. The iodine will cause a substance to turn to a dark blue color if it is positive for starch. The objective
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Biological Molecules Experiment-Specific Questions Experiment 1: Monosaccharide Test 1. Fill in the table below with the results from the monosaccharide test experiment‚ and your conclusions based on those results. Results Monosaccharide Test Solution Initial Color Color with Benedict’s Solution Color After Heating Monosaccharide? glucose solution clear blue orange yes water clear blue blue no sucrose solution clear blue
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T2:BIOCHEMISTRY AND ANALYSIS:DETECTION OF FATS‚PROTEINS AND CARBOHYDRATES OBJECTIVE The main purpose of the experiment is to understand some general tests that detect fats‚proteins and carbohydrates in foods. INTRODUCTION Carbohydrates are also known as sacharides. There are 4 main groups of carbohydrates‚which are monosaccharides‚ disaccharides‚ oligosaccharides and polysaccharides. Carbohydrates play an important role in living organism as it is the energy storage‚ and it also plays
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cracker solution‚ and cereal contain starch. In the reducing sugar test glucose‚ fructose‚ lactose‚ and milk contain a reducing sugar. In the grease spot test‚ salad oil‚ milk‚ and chips are lipids. In the dye test‚ salad oil and chips are fats. Egg albumin‚ cereal‚ and cracker mixture show that they are proteins. In the Spit lab the saliva and cracker test showed up positive. The starch test for saltine was positive. The reducing sugar test for saltine was
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atoms may include oxygen‚ nitrogen‚ sulfur…etc. They can be found in nature‚ since “organic” means present in‚ or derived from‚ plants and animals. Materials/ Apparatus: Bunsen burner‚ 3 test tubes‚ test tube holder‚ dropper‚ red litmus paper‚ albumin (cystine)‚ sugar (sucrose)‚ urea‚ soda lime‚ 1% sodium hydroxide‚ hydrochloric acid‚ 5% lead acetate Procedure A: Testing for Carbon‚ Hydrogen and Oxygen 1. 1g of sugar (sucrose) was placed in a dry test tube. 2. The test
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To examine three different ways of denaturing proteins. HypothesisIt was predicted that the physical characteristics of the egg white solution at room temperature would appear clear and normal like a raw egg white. This is because nothing would be done to the egg white. It was also predicted that when the egg white solution gets heated‚ the protein would denature if the temperature exceeds 65 °C. The protein would solidify‚ turn opaque‚ and turn white in colour. This would happen because the heat
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LEVELS IN HEN EGGS COLLECTED IN THE INDUSTRIAL AREA‚ COCHIN‚ SOUTH INDIA Thushara K. Sasi‚ Mithra IV‚ Sujitha E and Anbu Munusamy Dioxin Research Unit‚ National Institute for Interdisciplinary Science and Technology‚ Council of Scientific and Industrial Research (CSIR)‚ Thiruvananthapuran‚ Kerala‚ India 695019 ABSTRACT As a consequence of previous estimations by us of Eloor (Industrial area of Cochin‚ South India)‚ during 2008‚ dioxins and furans levels were determined in eggs from free range
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order to prepare paraffin sections (5 to 10 µm thick) thickness with ribbon formation. 3-4 sections length ribbon selected at 10 section interval‚ floated on the hot water bath at 500 centigrade for straightening and then picked up on the egg albumin (Mayer’s albumin) coated glass slides. The slides were allowed to dry in the warm plate at 400 C for 2 hours and stored. III.7 Staining Protocol: Deparaffinization: Slides with paraffin section were deparaffinized in xylene for 15 minutes (2-changes). Hydration
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time of the year; male gametes typically mature in the fall‚ and the female ovulation typically occurs in the spring time (1). Sperm storage allows females to be inseminated in the fall‚ hibernate for winter‚ and then successfully produce a cloth of eggs in the spring‚ with no need to go looking for a mate (1). This mechanism also allows females turtles an opportunity to indirectly increase their
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