undiluted uninduced is the darkest band out of the gel. Lane nine is the kaleidoscope which is a ladder with blood proteins and is a guild to show what proteins are in the gel. Lane ten has one band of IL-8 only. 32 ul undiluted induced‚ 8 reducing sample buffer 16 ul undiluted induced‚ 16 ul SDS‚ 8ul
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additional 15 minutes. The 65% cut was centrifuged for 15 minutes at 15‚000 xg. The supernatant was discarded. The pellet was resuspended in cold 4 mL of pH 7.5 phosphate buffer and transferred to a pre-chilled test tube for further purification. 200 uL was frozen down for later
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Cellular Biology Lab – Homework #3 Due the week of Nov. 4th You may use the lab manual‚ pre-lab lectures‚ and credible internet resources‚ however you may not use your cell bio lab classmates as a resource. You will most likely see this material again on the Final and I highly encourage you to work individually and seek help from myself or your TA. Plagiarism will result in an automatic zero. 1. In the cell bio lab‚ we use company manufactured gels‚ however you can make you own polyacrylamide
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The objective of the experiment was to differentiate mammalian C2C12 cells into muscle cells and analyze for presence of myosin light chain. Utilizing Western Blotting techniques and using specific antibodies helped to detect myosin light chain. The hypothesis was that proliferating myoblasts could be induced to initiate the differentiation process by depriving the cells of the necessary growth factors and that the differentiation is characterized by the expression of muscle-specific proteins. The
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University of Phoenix Material Foundations of Human Development Worksheet Write the key features‚ listed below‚ into the correct life stage of development and most significantly affected age range. In your own words‚ provide an explanation of the term and how it affects the developmental stage. An example is provided for reference. For the purposes of this assignment‚ field marked “N/A” do not need to be completed. Biological Development Psychological or Cognitive Development Social Development
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Religious Architecture: Comparative Study (Wells Cathedral and Friday Mosque‚ Isfahan) The purpose of this research is to compare and contrast two selected religious buildings‚ one of which Islamic‚ and another that is Christian. This will respectively exemplify and illustrate the development of Christian and Islamic styles of religious architecture before the end of the 14th century. The research will mainly focus on the importance of furniture design and interior decoration in each of the two
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OUTLOOK The future plans of Dar-ul-Sukun reaches beyond the borders of religion‚ cast‚ color and creed. The had always perform to make its members self-sufficient‚ so that they can play a productive part in the society. The non-discriminatory attitude towards the society is a big example of promotion of humanity and brotherhood. They have always provided extension platform to build a community around shared values‚ interest and passion to bring change to the society. Dar-ul-Sukun has tirelessly strived
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Culture of India From Wikipedia‚ the free encyclopedia Jump to: navigation‚ search Bharatanatyam‚ one of eight designated classical dance forms of India. The culture of India refers to the religions‚ beliefs‚ customs‚ traditions‚ languages‚ ceremonies‚ arts‚ values and the way of life in India and its people. India’s languages‚ religions‚ dance‚ music‚ architecture‚ food‚ and customs differ from place to place within the country. Its culture often labeled as an amalgamation of these diverse sub-cultures
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Christians and the rest is Muslims ; people in this country celebrate together the same holiday festivities whereas Christians have many holiday celebrations such as the head of the year ‚ and Muslims have two per a year such as ead feter and ead adha . And one of the best holiday celebrations ever was ead feter which we celebrate it for many reasons. One major reason of this holiday is to break the fast after thirty days without eating from the sunrise till sunset . And during this thirty
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