Genetic transformation of Escherichia coli with pGLO (Adapted from: Biotechnology Explorer: Bacterial Transformation: The pGLO System. Instructors Guide. BIO-RAD). Objectives a. To understand one of the most commonly used techniques for introducing DNA into E. coli cells and its use in molecular cloning. b. To become familiar with the concept of using green fluorescent protein (GFP) as a molecular tag for studying gene expression in bacteria and other organisms.
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The experimental membrane we used in this lab acted as a barrier in which only a small number of molecules can pass through. In this experiment we wanted to show the process of osmosis‚ in which water passes through the aforementioned barrier. Osmosis is critical to life because the process regulates
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Adwoa Fosuah Bio Psych Lab Report 4 Cold Pressor Task Aim The cold pressor test is done by having the subject submerge their hands in a bucket of cold water to determine cardiovascular changes such as blood pressure and heart rate. For this experiment we wanted to know how long participant can keep their feet in the bucket of ice water before they experience any pain while looking at neutral and positive pictures and also rate the intensity of their pain. For this reason we hypothesized that
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Date of Experiment: September 10th‚ 2013 Organic Chemistry II – CHLB330 Name: Symone E. MoxeyLab Partner: Lynden Cooper Synthesis of Aspirin (Acetylsalicylic Acid) Abstract:- Aspirin (acetylsalicylic acid) is produced experimentally in the lab. The resulting percentage yield is 65.5%. The purity of the obtained product is tested using the melting point and Ferric Chloride Test (FCT). The aspirin was massed‚ and the melting point was determined. Based on the data collected‚ there was a total
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Name Lab‚ Week #1 BALANCE LAB Introduction Several instruments are used for determining the mass of a chemical; these instruments are called “balances.” However‚ different balances have different measurements‚ meaning that some balances are more accurate than others. Moreover‚ there are several techniques that are used or practiced in order to obtain an accurate measurement. The two techniques are direct weighing and weighing by difference. Direct weighing simply means to read the mass
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Lab 9 Working with Printers Exercise 9.1 Installing a Printer Overview Contoso‚ Ltd. has just taken delivery of several new printers that the IT director purchased through an auction. He has assigned you the task of installing the printers and making them available to the users of the company network. For the first printer‚ you intend to connect the unit directly to an LPT port in the Windows 7 computer that will function as the print server. In Exercise 9.1‚ you install the driver for the printer
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“Dissections of Squalus acanthias and Rattus norvegicus” Ricardo Marin Biol 1107.103 (Tuesday 4-7) 12/05/2010 Miriam Hernandez Nayeli Perea I. Introduction The purpose of this lab is to be able to trace the flow of blood through both the mammalian and the shark heart. Learn all the major structures of the body systems of the shark and functions of certain structures as well as for the rat. Understand the terms of retroperitoneal‚ ovoviviparous‚ oviparous‚ and viviparous. Know which
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Lab 17 Amino Acids and Proteins Lab date 10/22/2013 12-1350 I Purpose The purpose of this experiment was to separate mixtures of II Method For a complete list of experimental procedures see prelab outline attachment #1. “For a complete list of experimental procedure see Seager‚ Spencer L. and Slabaugh‚ Michael R. Safety-Scale Laboratory Experiments for Chemistry for Today General‚ Organic and Biochemistry; Thomson Brooks/Cole‚ Belmont‚ CA‚ USA‚ 2008; pp.221-225”. III Data Part A Mass
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Flame Test Laboratory Report Introduction The purpose of this lab is to observe and identify metallic ions‚ using flame tests. The traditional flame test originated when Robert Bunsen invented the Bunsen burner to test two new alkali metals he and his partner had discovered. “A flame test is an analytic procedure used in chemistry to detect the presence of certain elements‚ primarily metal ions‚ based on each element’s characteristic emission spectrum” (Flame Test 1). Using this test‚ an element
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LAB 4 Read over the parts of the microscope and answer the following questions: 1. What do you call the lens you look through on the microscope? Ocular 2. What is the difference between the ocular lens and the objective lens? Ocular lens is the lens you look through and objective lens is the lens that is close to the stage. 3. Where do you place the slide on the microscope? the stage under the stage clips 4. Which adjustment‚ course or fine‚ do you use when you are observing the
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