One specifically important stain preformed was the Ex 3-7 Gram Stain‚ which showed the EI to be a gram-positive cocci that appeared in small clusters‚ in addition the endospore stain revealed this to be a non-endospore forming bacterial species. These results are important because they drastically narrow the possible bacterial species the EI could be. Many differential tests were performed on the EI‚ specifically differential tests that provide
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to be a Gram-positive cocci. This test eliminated all possible Gram-negative bacteria‚ Gram-positive rods and Gram-positive spirillium. Next‚ the endospore test determined whether or not the Gram-positive bacteria contained endospores. With the use of malachite green‚ steam‚ and safranin it was found that unknown bacteria 32 did not contain endospores. This eliminated Gram-positive cocci Sporosarcina spp. The oxidase test is a test using an Oxidase swab. The oxidase swab contains a chromagenic reducing
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inhabit the intestine of humans. It is normally a Gram-negative‚ rod-shaped bacterium. It is about to survive in physiologically high temperatures that allows up to excess of 50oC. (Daniel C‚ 2008). However it is not able to resist temperature like endospore. Therefore‚ the amount of bacteria would decrease as the heating persists. Germicides are disinfectants or chemicals used to kill germs which are mainly pathogenic microorganisms present in the environment. (Farlex‚ 2012) Germicides are formulated
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and 100°C for 2‚ 5‚ 30 and 90 minutes. As the same in experiment one‚ these cultures were then left for a week to incubate‚ then upon completion they were observed for distinguishable changes‚ such as the turbidity‚ clarity and the formation of endospores to show the bacteria’s survivability at particular temperatures. Information was then collated from other work benches to show the results from all five species. Results Table 1. Table of results showing the effect temperature has on the growth
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Microbiology Lecture Exam #1 Outline Chapter 1: Microbiology Introduction I. Microbiology Definition II. History of Microbiology (Christian Gram‚ Robert Koch‚ Antony van Leeuwenhoek‚ Robert Hook‚ Edward Jenner‚ Louis Pasteur‚ Elie Metchnikoff‚ Dmitri Iwanowski‚ Paul Ehrlich‚ Alexander Fleming) II. Scope of Microbiology A. Infectious Agents 1. Prions 2. Viroids 3. Viruses B. Prokaryotes
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Exercise 8-B Differential Staining Gram Staining and Acid Fast Staining Introduction: Differential Staining‚ one which facilitates differentiation of various elements in a specimen is a general term that can refer to a number of specific processes. Using multiple stains can better differentiate between different microorganisms or cellular components of a single organism. Gram’s Stain is a widely used method of staining bacteria as an aid to their identification. It was originally devised by Hans
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Bio 225 Chapter 4 Practice Questions 1. Which of the following is not a distinguishing characteristic of prokaryotic cells? a. Their DNA is not associated with histones. b. They have cell walls containing peptidoglycan. c. They lack membrane-enclosed organelles. d. Their DNA is not enclosed within a membrane. e. None of the above. 2. Which of the following is not true about a gram-positive cell wall? a. It contains teichoic acids. b. It protects the cell in a hypertonic environment
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surface of some bacteria Halophile- Salt loving Achaea that live in environments with very high salt concentration Prokaryote- single celled organisms‚ lack membrane bound nucleus Zoonosis- A disease that can be passed down from animals to humans Endospore- When Gram positive bacteria can form a thick coated‚ resistant structure Compare and contrast Gram positive and Gram negative bacteria. Gram negative- Dyes red its more complicated and has less peptidoglycan Gram positive- Dyes purple its less
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Name Microbiology 2210 Answer Key Practice Test 1 Directions: Choose the most correct response to answer the statement. ___1. Archaea a. belong to the kingdom Protista b. have petidoglycan in their cell walls c. belong to the domain Bacteria
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Name and Course Section: Avital Gershtein‚ Section 701 Title: Aseptic Technique & culturing Microbes - Lab # 4 Purpose: The purpose of this experiment is to Learn and employ aseptic technique‚ to become familiar with basic requirements of microbial growth‚ to learn the basic forms of culture media‚ and to become familiar with methods used to control microbial growth. Bacteria Growth pattern after 24 hours Growth pattern after 48 hours L. acidophilus in liquid MRS broth No growth Amount of sediment
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