staining procedures were performed as needed. Unknown A required further endospore staining‚ whereas unknown B required no further staining procedures. Once all staining methods were complete‚ specific biochemical tests were performed. The biochemical tests were chosen from the unknown identification tables and dichotomous keys that were given in the lab manual. Since unknown A was determined to be a Gram positive rod and an endospore producer‚ the genus Bacillus was then concluded. Unknown A bacteria
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specifically called ________. Question 11 2 out of 2 points A bacterial genus that has waxy my colic acid in the cell walls is ________. Question 12 2 out of 2 points The function of bacterial endospores is ________. Question 13 2 out of 2 points Endospores are ________. Question 14 2 out of 2 points Which of the following is not true of the outer membrane? Question 15 0 out of 2 points A bacterial cell exhibiting chemo taxis probably has ________
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Review Sheet Exercise I: Survey of Higher Microorganisms: Protozoa‚ Fungi‚ and Helminths Protozoa (group of Kingdom Protista) 1. Amoeba a. nucleus- dark center of the cell b. food vacuole- They feed by taking nutrients into the cell by diffusion and packaging it into (clear circles spread throughout the cell) c. pseudopod- “false foot”; the motility results from the streaming of the protoplasm that forms the process 2. Entamoeba causes amoebiasis or amoebic dysentery‚
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Materials and Methods: Inoculation of the unknown was done on a nutrient slant agar The nutrient agar was then incubated at 27°C for one week. The agar appearance was observed and recorded. There was growth that appeared pinkish in color. The first test we did was basic stain using a heat fixed emulsion which kills the bacteria and allows them to adhere to the slide and thickens their protein for better staining. I then covered the heat fixed emulsion with crystal violet for one minute. The stain
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Chapter 17 Louis Pasteur’s experiment illustrated that: microbes will not grow in a nutrient broth that has been sterilized unless air is allowed to enter the vessel through an opening. Living organisms can spontaneously generate from nonliving matter. True or False? False The scientists usually given credit for disproving the theory of spontaneous generation of bacteria are: Tyndall & Pasteur Miller and Urey’s experiments attempting to recreate the prebiotic environment produced a rich mixture
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temperature required to destroy many species of bacteria‚ fungi‚ viruses and yeasts is approximately 60C (van Doornmalen‚ 2008). However‚ some bacterial species contain endospores that are known to be thermoduric. When a bacteria is thermoduric‚ it is able to withstand prolonged exposure to temperatures up to an around 100C. Many endospore-forming bacteria are well known for the possible pathological dangers they hold. Therefore‚ it is vital develop methods by which these bacteria are effectively and
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Bacillus‚ Mycoplasma‚ and Escherichia In a clinical setting signs and symptoms can lead a care taker down a treatment path‚ but if a bacterial infection is suspected‚ a definitive answer from the “lab” is needed before prescribing medications. In this instance a yellow sputum was produced by the patient‚ which will be taken to the lab an analyzed under a microscope. There are three suspect bacteria‚ which are Bacillus‚ Mycoplasma‚ and Escherichia. A discussion of staining procedures‚ as well as
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Gram Stain: The important part of this experiment is being able to determine a bacterium based on its cell wall structure. It also helps indentify if the unknown organism is a Gram positive or Gram negative. This is the initial step that must be taken before any other lab procedure may continue on to ensure the purity is present‚ the arrangement of the cells‚ and the shape the cell has. The staining of the cell starts off with using the primary stain‚ Crystal Violet which is a purple color‚ to begin
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Shakira Jarvis Microbiology Lab Assignment Laboratory Assignment Outline 1. Check in & The Microscope a. Review of proper lab etiquette. i. Review laboratory syllabus and b. Review of the Parts of a Microscope ii. Review of lab exercises about different types of Microscopes 2. Survey of Microorganisms c. Viewing‚ drawing‚ and describing several types of fungi‚ algae‚ and Bacteria iii. Chlamydomonas iv. Spirogyra
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Microbiology Exam 1 Name_______________________ 1/30/07 1. (1 pt) Who was the first person to observe bacteria using a microscope? a. Lister b. van Leeuvenhoek c. Pastuer d. Koch 2. (2 pts) Which two of the following contribute to the opportunistic and infectious nature of bacteria? a. flagella b. ability to persist in unfavorable environments c. selectively permeable membranes d. fast growth e. ability to sense chemical gradients f. peptidoglycan 3. (1 pt) Capsules‚ sheaths‚ and
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