Transformation of E. coli by plasmid DNA 1. Table showing the results from the selective plates |Plate |Plasmid |Contents of plates |Number of colony | | | | |White |Blue | |1 |Ligation mixture |Ampiclillin + X- gal + IPTG |10 |0
Premium Transformation Trigraph Escherichia coli
Research Question How will the addition of different pH buffers to amylase affect the rate of starch digestion measured using starch and iodine? Introduction Amylase is an enzyme found in human saliva and pancreas. It is the digestive enzyme that is needed to breakdown starch molecules. Amylase must be kept at certain conditions to function at its optimum level. This experiment will explore the effect of pH (1‚ 4‚ 7‚ 10‚ and 14) on the function of amylase by using starch and iodine. Usually
Premium Enzyme PH Buffer solution
Determination of presence of protein in a variety of food substances by the use of Biuret Reagent Biol. 1040 Section 1005 Introduction The human body consumes carbohydrates‚ lipids‚ and proteins each day by eating a variety of foods. These foods are required for energy and human growth. Proteins are a diverse group of macromolecules with many different functions (Mbuthia‚ 2012). Many are structural components of bone‚ muscle‚ hair‚ tissue‚ etc. Others are enzymes that speed up cellular
Premium Protein Amino acid
Page 1/3 09/2010 SE 110.22 Forces in an Overdeterminate Truss The picture shows SE 110.22 in a frame similar to SE 112. * Comparison of forces in statically determinate and overdeterminate trusses1 * Bars with strain gauge full bridges to measure bar force1 * Computerised evaluation of experiments Technical Description Overdeterminate trusses are employed where overdimensioning is purposely required because safety must be maintained in the event of failure of an element‚ such as in aircraft
Premium Beam Force Truss
Purpose To check whether mass is gained or lost during a Chemical reaction. Hypothesis Equipments and Materials • Eye protection • Test tube • 250 ml Erlenmeyer flask and stopper • Weight Balance • Antacid tablet • Dilute solutions of o Sodium Hydroxide‚ NaOH(aq) o Iron(III) Nitrate‚ Fe(NO3)3 • Funnel • Cylinder Procedures 1. Put eye protection on for safety purposes. Part A: Reaction between Iron(III) Nitrate and Sodium Hydroxide. 2. Take two cylinders and fill one (full)
Free Sodium hydroxide Chemistry Sodium
Experiment #2 “Density Determinations” Report The objective for experiment #2 was “to determine densities of objects/salt solutions with different concentrations of salt‚ to see how density changes as a function of concentration.” In experiment #2‚ part II‚ calculations of Density of NaCL solutions were made from 0%-25% NaCL concentration. My hypothesis was that as the % increased‚ so would the density‚ because adding weight would increase the density of each solution. The density calculations in
Premium Measurement Density Standard deviation
In November 1994‚ John Wells‚ a customer service representative of Bayfield Mud Company‚ was summoned to the Houston warehouse of wet land Drilling‚ Inc.‚ to inspect three boxcars of mud-treating agents that Bayfield Mud Company had shipped to the Houston firm. (Bayfield’s Corporate Offices and its largest plant are located in orange‚ Texas‚ which is just west of the Louisiana-Texas border.) Wet-land Drilling had filed a complaint that the 50 Pound bags of treating agents that it had just received
Free Weight Mass Bag
Second (s) 2. Give the correct SI derived units of the following quantities. Density Speed Acceleration Force Pressure kg/m3 Energy 3. Convert the following: μm (Ans. 1.22) a. 1.22 x 10-9 km = b. 6.523 x 10-4 nm = c. 2.5x 10-9 nm3 = d. 30.5 cm/s = e. 9.6 ng/mm2 = 4. Mm (Ans. 6.523 x 10-19) mm3 (Ans. 2.5 x 10-27) km/h (Ans. 1.09 x 106) kg/m2 (Ans. 9.6 x 10-6) Perform the following calculations and report each answer with the correct number of significant figures and units. b. 2.457
Premium Kilogram Gold Mass
carbon dioxide‚ helium‚ and lab gas. We took a Ziplock bag‚ and turned into a non-stretchy balloon‚ and filled with each gas and measured the apparent mass then calculated the actual mass‚ then find a ratio between the mass of oxygen and the other gasses. We tried to keep the pressure about the same each time so our calculations would be more accurate. We found out that the heaviest was carbon dioxide‚ and the lightest was helium. In fact‚ helium and lab gas had a density below 0. Anyways‚ it turned
Premium Oxygen Carbon dioxide
A Report into the concept and effects of Merchandise Display Densities 1. Define the Concept of store merchandise display densities. Store merchandise display density describes the amount of product per foot on display. A foot is the approximate size of the display rail‚ it is perceived as a unit‚ rather than a measurement. The product is counted in styles‚ the unique design of the product‚ and options‚ its available colour variations. Primark has on one foot only one stile in one option
Premium Clothing Decision making Unsolved problems in neuroscience