Purpose: To identify an unknown bacterial specimen using basic laboratory technique and biochemical tests. The unknown bacteria will be one of the following: Enterococcus faecalis‚ Staphylococcus saprophyticus‚ Escherichia coli‚ Enterobacter aerogenes‚ Proteus vulgaris‚ Salmonella [I assume typhimurium]‚ or Shigella [either flexneri or sonnei‚ we used both in our lab during the semester]. Procedure {and observations}: Observe bacterial colony morphology. {Colonies are large‚ beige or
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Microbiology UNKNOWN LAB REPORT Kateryna Petrakova INTRODUCTION Microorganisms cause a great amount of diseases. For healthcare providers it is very important to be aware of what organisms are pathogenic and cause a disease and‚ therefore‚ to find an appropriate treatment. Different microorganisms require various environments in order to replicate and to become dangerous for a person’s health. An integral part of any medical treatment is to be able to recognize and
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medium is also known as an "indicator medium" and a "low selective medium". Absence of electrolytes serves to inhibit swarming by Proteus species. Lac+ By utilizing the lactose available in the medium‚ Lac+ bacteria such as Escherichia coli‚ Enterobacter and Klebsiella will produce acid‚ which lowers the pH of the agar below 6.8 and results in the appearance of red/pink colonies. Eosin methylene blue (EMB) agar – Eosin methylene blue (EMB) is a selective stain for Gram-negative bacteria.
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Discussion The possible identity of the unknown organisms in the mixed culture was limited to bacteria that we had worked with previously in lab. Initially a Gram stain was conducted in order to distinguish the unknown bacterium as a Gram-positive and/or a Gram-negative organism (Lancaster and Bennett‚ 2012; Kellenberger‚ 2001). Based upon the results‚ both Gram-negative and Gram-positive bacteria were observed in the unknown mixed culture (Table 1 and Table 2; Kellenberger‚ 2001). In order
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Out of 28 Unknown microorganisms‚ slant 6O was randomly selected‚ and subjected to testing in order to identify it’s species. Seventeen of the unknown bacteria were classified as Gram-negative bacilli: Corynebacterium pseudodiphtheriticum‚ Enterobacter aerogenes‚ Eschirichia coli‚ Flavobacter capsulatum‚ Helicobacter pylori‚ Klebsiella pneumonia‚ Klebsiella oxytoca‚ Proteus hauseri‚ Proteus mirabilis‚ Proteus vulgaris‚ Pseudomonas aeroginsoa‚ Alcaligenes faecalis‚ Rhizobium radiobacter‚ Salmonella
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Unknown Lab Report Dr. Nathan Cahoone Microbiology 204 December 9‚ 2010 Introduction There are many reasons for knowing the identity of microorganisms. The study and test was done by applying all of the methods that have been learned so far in the microbiology laboratory class for the identification of an unknown bacterium which I was using unknown #25. Results Unknown #25 had the following morphology on a streak plate: medium sized butyrous cream colored colony. Gram-staining was utilized
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The results show that the unknown and E. aerogenes were positive for this test while P. aeruginosa was negative. Results for P. vulgaris were negative for this test‚ but this bacteria can show positive or negative for the Ornithine‚ The lactose fermentation test (MAC) showed that the unknown was
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Title: Catalase enzyme detection Objective: To understand the function of catalase in cells that produce the enzyme‚ interpret the results of a catalase test and know their value in differentiating bacteria. Materials: 1 clean microscopic slide‚ 3% H2O2 solution‚ swabs. Micrococcus luteus‚ Enterococcus faecalis‚ patient G Procedure: 1) Scrape some cells off from each bateria to the slant and place them on glass slide. 2) Place one or two drops of H2O2. Watch for bubbling as an indication of
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pp. vii of the lab manual for more information on academic misconduct. TASK: This assignment is based on your investigation on the tolerance of a bacterium (Enterobacter aerogenes) to a saline gradient. Use the pooled data for your lab day (posted on D2L) to complete this assignment. FORMAT: The page limit for this assignment is 3
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Name: Madison Gullatt Sect/Table: !/D Experiment 8 Report: Extracellular Enzymes Purpose: The purpose of this experiment is to perform a variety of different test and record the data from each test in relation to extracellular enzymes. Results: 1. Fill in the following tables with your observations. Note if the test is positive or negative for the enzyme. Also note the appearance of the colonies and/or media. This will include: the color of the medium immediately surrounding
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