bacteria culture using differential tests. The identification of the unknown culture was accomplished by identifying the bacteria based on its specific metabolic characteristics and morphology. It is suggested that culture 11 is a sample of Enterobacter aerogenes. Introduction: This experiment was centered on metabolic and biochemical testing procedures. The rationale of performing these tests was to distinguish six different microbes from one another and to compare how their metabolic and biochemical
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food spoilage(Other than Canned Foods) with some examples of causative organisms Food Type of Spoilage Microorganisms involved Bread Mouldy Rhizopus nigricans Penicillium Aspergillus niger Ropy Bacillus subtilis Maple sap and syrup Ropy Enterobacter aerogenes Yeasty Saccharomyces Zygosaccharomyces Pink Micrococcus roseus Mouldy Aspergillus Penicillium Fresh fruits and vegetables Soft rot Rhizopus Erwinia Gray mold rot Botrytis Black mold rot A. niger Pickles‚ sauerkraut Film yeasts‚ pink
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References: 1. R. S. Horvath and M. E. Ropp. 1974 “Mechanism of Action of Eosin-Methylene Blue Agar in the Differentiation of Escherichia coli and Enterobacter aerogenes”.International journal of systemic bacteriology. April 1974‚ p. 221-224 2. Dagny Jayne Leininger‚ Jerry Russel Roberson‚ Franc¸ois Elvinger. 2001 “Use of eosin methylene blue agar to differentiate Escherichia coli from othergram-negative mastitis
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Pure Culture Techniques In this first lab‚ you will be learning some very fundamental and important techniques. As is the case with most things‚ shorts cuts usually get you in trouble. This is especially true in Microbiology. The techniques you will be learning tonight‚ if mastered correctly‚ will make your life and learning experience in Microbiology much easier‚ if you don’t pay attention and practice these techniques incorrectly‚ well then……? Today you will be learning the following techniques:
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HOW TO WRITE AN UNKNOWN LAB REPORT IN MICROBIOLOGY GENERAL Unknown reports in microbiology are written in scientific format. Scientific writing is written differently from other types of writing. The results of the exercise or experiment are what are being showcased‚ not the writing. The purpose of scientific writing is not to entertain‚ but to inform. The writing should be simple and easy to understand. There is a specific style that must be followed when writing scientific reports.
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Determining an Unknown Through Deferential Stains and Biochemical Tests Introduction There are many reasons for knowing the identity of microorganisms. The reasons range from knowing the causative agent of a disease in a patient‚ so as to know how it can be treated‚ to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that have been learned so far in the microbiology laboratory class for the identification
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antibiotic zone size in the chart‚ determine if it is sensitive‚ intermediate‚ or resistant; and report to TA. Procedure: Selective and Differential Media 1. Label the MacConkey agar plates. Streak the plates for the following organisms: Enterobacter aerogenes‚ Salmonella enterica‚ and Staphylococcus aureus‚ and sterilize the loop after streaking each quadrant. 2. Label a sheep blood agar plate and use a loopful of Enterococcus faecalis to inoculate it. Streak the plate. 3. Repeat step
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Determining the Unknown Microorganism from a Mixed Culture Mylah Ferland‚ Rivier University MICRO 206‚ March 16th‚ 2016 Introduction: An unknown bacterium experiment‚ this experiment was done to discover what bacterium was in a mixed culture sample. This experiment worked with enteric bacteria which are members of the Enterobacteriaceae and live in the intestinal tract (Willey et al.‚ 2014). Also found in Willey et al (2014) is some characteristics of enterics such as they all degrade sugars by
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Exercise 5 Introduction: Many different species of bacteria look similar under the microscope and also have the same staining results (ex. Gram stain). To be able to differentiate between the different species‚ one can look at the metabolic differences (fermentation)‚ as well as the environmental condition differences (temperature‚ pH‚ oxygen requirements). Being able to manipulate these conditions in a controlled environment can help to correctly identify the exact bacteria. Different media
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MICROBIOLOGY 3444-007 Gram Negative Unknown Unknown Bacteria #14 LeNaiya Kydd 4/2/2014 Abstract In order to be able to identify the unknown organism that was given to us‚ we had to conduct a number of different tests. These biological tests are used because they help us be able to identify the properties of the unknown we have and be able to compare our observed results with actual results of all the potential organisms. When all of the data of the test are put together it is easy to
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