Identification of A Mixed Culture Unknown An experiment such as this one serves the purpose of allowing us‚ the students‚ to apply what we already know about any organism and any laboratory procedure to the difficult task at hand. It is possible to identify a mixed culture by running familiar experiments on the unknown bacteria and taking information already known about specific bacteria and applying it to the results. This helps to slowly eliminate any bacteria that do not correspond with the
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As a result of the tests ran‚ I was able to identify the unknown bacteria. For Unknown A‚ I ran six tests. I first isolated the bacterium from the second bacterium and found a clear growth (Table 1‚ Figure 1). Secondly‚ I ran a gram stain and found a gram positive‚ cocci bacterium (Table 1‚ Figure 2). Third‚ I ran a catalase test in which was negative (Table 1). From here‚ I determined a starch hydrolysis test would be necessary to distinguish between different bacteria. The result was negative (Table
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Biochemical Test – Enterobacteriaceae Bacterial spp incapable of fermenting glucose cannot utilize lactose. 2 enzymes necessary for a bacterium to take up lactose: A. β-galactoside permease – facilitates the entry of lactose molecules to bac cell wall B. β-galactosidase – breaks down lactose into β-D glucose and β-D galacatose LF possess both enzymes NLF do not possess β-galactosidase LLF do not possess β-galactoside permease Glucose fermenters only (true enteric pathogen)
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treat and cure the symptoms. Introduction In a lab today‚ I am to identify an unknown bacterium that is causing a patient lower abdominal pain. There are six different organisms that the unknown bacteria could be. They are Eschericha coli‚ Enterobacter aerogenes‚ Klebsiella pneumoniae‚ Proteus mirabilis‚ Pseudomonas aerogenosa‚ and Salmonella typhimurium. I will be using several different types of biochemical testing. I am using this type of testing because it differentiate and sometimes identify
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iLab #2 Carbohydrate Tests INTRODUCTION - For this experiment we will be testing four different bacteria with four different tests‚ using glucose‚ lactose‚ and sucrose. Hopefully we will use the information from those test to be able to identify the organisms in each of the samples from the case studies. We will use the results from the four different tests along with the information of how different bacteria react to match up to the case
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Food Fermentation Microbiology sasimalani@msu.edu.my INTRODUCTION • Industrial microbiology uses microorganisms‚ typically grown on a large scale‚ to produce valuable commercial products or to carry out important chemical transformations. • This process is commonly referred to as Fermentation DEFINITION OF FERMENTATION “The process of deriving energy from the oxidation of organic compounds‚ such as carbohydrates‚ using an endogenous electron acceptor‚ which is usually an organic
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Introduction To date‚ the bacterial resistance to antibiotics is one of the most important global health threat introduced by the World Health Organization (WHO) and most annual mortality due to hospital infections occurs because of this challenge (1). According to the US Centers for Disease Control and Prevention (CDC) report‚ carbapenem-resistant Enterobacteriaceae (CRE) are one of the three main antibiotic resistance threats. (2). Enterobacteriaceae are Gram-negative bacilli which cause a wide
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Bacterial Fermentation Secondary article Article Contents Volker Mu¨ller‚ Ludwig-Maximilians-Universita¨t Mu¨nchen‚ Munich‚ Germany . Introduction Under anaerobic conditions‚ in the dark and in the absence of electron acceptors‚ organic compounds are catabolized by strictly anaerobic or facultatively anaerobic bacteria by internally balanced oxidation–reduction reactions‚ a process called fermentation. In fermentation‚ the organic compound serves as both electron donor and acceptor‚ and adenosine
Free Cellular respiration Adenosine triphosphate
Microbial Diversity and Ubiquity Microorganisms are microscopic organisms that are so small that that they can only be visualized by the aid of a compound-brightfield microscope. While we generally cannot see individual microorganisms with the naked eye‚ they are present in virtually every habitat known to man. Microorganisms can be prokaryotic—the bacteria or eukaryotic—the algae‚ protozoa or fungi. While viruses are acellular they are also studied in the scope of microbiology because
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David Kennedy Bio 210 Lab Report 1 10/11/13 Microbial Growth Background Information: This lab was conducted in order to understand basic differences among differential and selective media‚ while recognizing how each media is used to isolate and identify microorganisms (Wistreich‚ 2003). The first microorganism analyzed was Staphylococcus epidermidis. This organism is gram-positive‚ single celled‚ arranged in grape-like clusters‚ and cocci in shape (Bukhari‚ 2004). S. epidermidis is approximately
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