within the mucous coating on the intestine. They don’t damage their range-land. Dr. Gilmore along with B. Faecalis‚ the research exhibited "that it devotes many of its genetic material to digestive enzymes in which take place on the surface of the cell.” That suggests it can be breaking substances for other additional organism‚ most likely aiding certain sorts of microbial neighbors. Enterococcus might be getting the chance to move nearer to the small intestine or even the urinary tract that is to
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REVIEW OF RELATED LITERATURE This investigatory project aims to make use of rambutan peeling extracts that is high in phenolic compounds such as tannin and saponin that exhibits antioxidant activity and antibacterial activity against pathogenic bacteria. Although the seeds contain such compounds the peeling exhibit greater amount of the said compounds. Rambutan is a very popular fruit cultivated in the Philippines and it is eaten in large amounts. The rambutans rind is quickly discarded and
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Introduction The main purpose of root canal treatment is the elimination of microorganism from root canal system (1‚2). Microorganisms and their byproducts are regarded as the primary etiological factors of periapical diseases and pulpal necrosis (3‚4‚5). Microbial reduction obtained by mechanical instrumentation‚ irrigation‚ using intracanal medicaments and adequate filling of root canal (6). Failure of the root canal treatment is the result of persisting microorgansims in the root canal system
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The Results from the Gram positive tests indicated that the unknown #4 was Streptococcus pyogenes. All seven tests on the unknown matched S. pyogenes perfectly. The blood agar plate proved the unknown to be β hemolytic‚ meaning the unknown bacteria was capable of complete hemolysis. This test separated the unknown into the β Streptococcus group‚ narrowing the possible bacteria to S. aureus or S. pyogenes. The Catalase test was used to determine if the unknown could break down hydrogen peroxide
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quantification of indigenous intestinal microbiota. The success of such analysis requires effective methods for the extraction of faecal bacterial DNA. Three extraction methods were assessed for their effectiveness in extracting Escherichia coli and Enterococcus spp. bacterial DNA from human faecal samples: an in-house phenol/chloroform extraction‚ and two commercially available kits‚ ExtractMaster (Epicentre Biotechnologies) and UltraClean (Mo Bio Laboratories) faecal DNA extraction kits. Real-time PCR
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Pure Culture Techniques In this first lab‚ you will be learning some very fundamental and important techniques. As is the case with most things‚ shorts cuts usually get you in trouble. This is especially true in Microbiology. The techniques you will be learning tonight‚ if mastered correctly‚ will make your life and learning experience in Microbiology much easier‚ if you don’t pay attention and practice these techniques incorrectly‚ well then……? Today you will be learning the following techniques:
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Oxygen Requirements of Bacteria BACKGROUND The GasPak system is useful for culturing anaerobic bacteria on standard microbiological media because the GasPak generates carbon dioxide and hydrogen. The hydrogen will combine with oxygen present in an anaerobic jar to produce water. This system can reproducibly attain oxygen levels in the parts per million range if used correctly. This is the best method for determining the oxygen requirements of unknown organisms. A candle jar is useful
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who has previously damaged cardiac valves E. faecium may lead to endocarditis. Although Enterococci are part of the normal flora in humans and animals there are only a few of its species that cause clinical infections. The most common being Enterococcus faecalis‚ which accounts for up to 90% of clinical isolates. However the other species‚ E. faecium‚ particularly the vancomycin-resistant strain that was once nearly 10%‚ is beginning to increase in the amount of clinical isolates. This increase may
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Lab Report: Antibiotic Evaluation by the Kirby-Bauer Method Introduction Chemical antimicrobial agents are chemical compounds capable of either inhibiting the growth of microorganisms or killing them outright. Those which are taken internally to alleviate the symptoms of or promote healing from disease are called chemotherapeutic drugs‚ and among these is a class of compounds called antibiotics. In order for a chemotherapeutic drug to be classed as an antibiotic‚ it must be produced by
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Lab Management Assignment Hand Sanitizer 2012 xxx [Pick the date] HAND SANITISER * * A hand sanitizer or hand antiseptic is a supplement or alternative to hand washing with soap and water. * Various preparations are available‚ including gel‚ foam‚ and liquid solutions. * The active ingredient in hand sanitizers may be isopropanol‚ ethanol‚ n-propanol‚ povidone-iodine. * Inactive ingredients in alcohol rubs typically include a thickening agent such as polyacrylic
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