DISCUSSION In this experiment‚ we will extract plasmid DNA that carrying pKan and pAmp from E. coli that have been cultured overnight in LB media containing antibiotic kanamycin and ampicillin respectively. So‚ the DNA that will be introduced later will make the bacteria resistance to antibiotic kanamycin or ampicillin. A plasmid is a small‚ circular‚ double stranded DNA molecules and cloning vector that are widely used for recombinant DNA technology. It can be physically separated from chromosomal
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Investigation of action of saliva and hydrochloric acid in two carbohydrate solutions. Objective: To determine the action of amylase and hydrochloric acid in two carbohydrates solutions Apparatus and equipments: 1. Boiling tube 2. Metal test tube racks 3. Beaker 4. Graduated plastic dropper 5. Water bath‚ ~37ᵒC 6. Water bath‚ ~95ᵒC Materials: 1. Carbohydrate solution A 2. Carbohydrate solution B 3. Benedict’s solution 4. 3M Hydrochloric acid 5. 3M Sodium hydroxide Procedures: 1. Two boiling tubes
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this experiment‚ t-pentyl chloride was synthesis by reacting concentrated hydrochloric acid using nucleophilic substitution sn1. In the substitution reaction the Hydrogen in HCl protonated the alcohol group in t-pentyl alcohol and turned it to a good leaving group (H2O). a tertiary carbon cation was formed. a nucleophilic attach of the negative Cl- attacked the carbon cation forming t-pentyl chloride [7]. As the reaction proceeded‚ extraction and distillation techniques were used to get a pure product
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b) Write the anode and cathode half-reactions‚ and the net cell reaction for the above diagram. [ T/I / 2 ] Oxidation: Zn (s) Zn2+ (aq) + 2e- Reduction: Cu2+ (aq) +2e- Cu(s) Zn (s) + Cu2+ (aq) +2e- Zn2+ (aq) + 2e- + Cu(s) Zn (s) + Cu2+ (aq) Zn2+ (aq) + Cu(s) c) Write the cell notation for the above galvanic cell drawn. [ T/I / 1 ] Cu(s) | Cu2+(aq) || Zn 2+(aq) | Zn (s) d) Calculate the percent error of the cell potential for the above galvanic cell. Show all
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Report Submitted: June 12‚ 2013 Title: Observations of Chemical Changes Purpose: The purpose of this experiment is to predict and examine chemical changes/reactions among common chemicals found in consumer products at a macroscopic level. Procedure: The procedure of this experiment was to carefully mix different chemicals together using the chemical filled pipettes and the 96-well plate and determining what chemical reactions occurred. Data Table: Observations of Chemical Changes Experiment
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This is then reduced by means of iron powder or sodium sulphide to get para-aminophenol. The recovery of para-aminophenol is completed by dissolving the para-aminonphenol hydrochloride salt in water‚ decolourising the aqua solution and crystallising the para-aminophenol by neutralizing the salt. The resulting para-aminophenol is reacted with acetic anhydride to produce paracetamol. The reactions are summarized as shown below: Process using Paranitrochlorobenzene
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chemical reaction that will turn the liquid in the intestine (Dialysis tubing) blue-black. If the amylase successfully digests the starch‚ what results would you expect? If the amylase successfully digests the starch‚ I would expect a no color change occur as well as a presence of glucose in the intestine (Dialysis tubing). If the amylase only partly digests the starch‚ what would you expect to happen? If the amylase only partly digests the starch‚ I would expect to see a faint color change as
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experiment the objective was to perform a Diels-Alder reaction using cyclopentadiene and maleic anhydride to synthesize the product‚ cis-Norbornene-5‚6-endo-dicarboxylic anhydride. The Diels-Alder reaction is one of the most important reactions in organic chemistry and was first investigated by Otto Diels and Kurt Alder in Germany. It is a [4+2] concerted cycloaddition reaction which involves a diene and a dienophile. The Diel-Alder reaction are mainly used for creating new carbon-carbon bonds‚ mostly
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Report Submitted: September 4‚ 2013 Title: Observations of Chemical Changes Purpose: To observe some properties of chemical reactions and to associate chemical properties with household products. Procedure: In this experiment I used a 96-well plate to hold each of the chemical reactions that occurred during this lab. Using a pipet‚ I added two drops of one chemical with two drops of another chemical and observed the effects of each reaction. Data Table 1: Experimental Data Well # |
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DKFLSDFKLFKJ | Acid-Base Titration. | kfjhdkjhvdkfj | | April Jowers | 12/19/2012 | DKFJSDKJFHDSKJHF | Introduction In this lab we will use basic titrating skills and techniques in order to titrate HCl. We will also be practicing how to prepare the solution. Using the titration data‚ we can practice our stoichiometric skills and also become more familiar with using lab equipment. Titration is the process of measuring the exact volume of a solution of known concentration that is
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