Enzyme Catalysis Lab: Laboratory Write Up Problem/Question: What happens to the amounts of gas produced in a catalyzed reaction of Hydrogen Peroxide when the catalyst is mixed with an acid? Theory/Hypothesis: In this experiment the researcher and their team will be performing tests on a catalyst (proteins found in potato) to see what will happen when the catalyst in a Hydrogen Peroxide reaction is treated with an acid before the reaction occurs. The researcher hypothesizes that the after treating
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that assays of alkaline phosphatase activity in homogenised tissue samples will give better responses if both Mg2+ and Zn2+ ions are included in the reactions Keywords: Alkaline phosphatase; kinetics; Enzyme-cofactor interaction; synergism * corresponding author. Email: femijohn@gmail.com 43 INTRODUCTION The roles of metal ions in metalloenzymes include direct participation in catalysis‚ stabilization of protein structure and regulation of enzymatic activity. Membrane alkaline phosphatase (ALP)
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Discussion: From the experimental data‚ the concentration can be found. There were a few discrepancies during the experiment which could throw off the data but in all everything went correctly. Discussion for errors: There were some parts during the experiments that the group could not distinguish when the color change occurred so there was more solute
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Sang Kim Enzyme Catalyst Purpose/Problem: There are four parts to the Enzyme Catalyst lab - Activity A‚ B‚ C‚ and D. In activity A‚ the characteristics of enzyme actions will be observed. The main purposes are to determine the rate of an enzyme catalyzed reaction‚ to study the characteristics of an enzyme mediated reaction‚ and to observe the effect of heat on enzyme activity. The purpose of activity B is to use the Titration Protocol to determine the initial amount of H 2O2 present
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What is Enzyme? Enzymes are protein that acts as speed up reactions and break down molecules in our body. However‚ different enzymes only work on certain types of molecules. Enzymes can accelerate the reactions by more than one million times.(3) In our human body‚ there are a total about forty thousand types of enzymes and each catalyzes different kind of molecule.(3) The molecules that enzymes help to accelerate is called substrates‚ and when enzyme is combined together with the substrate‚ it
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CHAPTER 4: ENZYMES Enzymes are biological catalysts. There are about 40‚000 different enzymes in human cells‚ each controlling a different chemical reaction. They increase the rate of reactions by a factor of between 106 to 1012 times‚ allowing the chemical reactions that make life possible to take place at normal temperatures. They were discovered in fermenting yeast in 1900 by Buchner‚ and the name enzyme means "in yeast". As well as catalysing all the metabolic reactions of cells (such as respiration
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Structure: Enzymes are globular proteins that act as catalysts‚ they have a specific 3D shape that is the result of their amino acid sequence. There is a specific region of the enzyme that is functional‚ this is called the active site. The active site is made up of a small number of amino acids and forms a small depression within the larger enzyme molecule. Moreover‚ the molecule that the enzyme acts upon (substrate) fits precisely into the depression and forms an enzyme-substrate complex. The substrate
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Conclusion Following the countries accession to the World Trade Organization‚ financial liberalisation has picked up considerable momentum. Compared to other studies on financial liberalisation‚ this study focuses on the impact of financial liberalization on Vietnam’s stock market. After collecting data‚ calculating and running models‚ in comparision with previous studies‚ it shows us that there are some points to be mentioned as following. For the contribution to the literature‚ there is very
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in these reactions‚ resource‚ products and enzyme are three most mainly things. In all chemical reactions‚ there is a activation energy(EA) needed for them. However‚ if the rate of reactions are too low‚ they will not be able to sustain lives. In this case‚ they have enzyme as a protein catalyst. As reaction catalysts‚ enzymes are proteins that adjust the process by initiating the reaction. In this case‚ there are also many elements that influence enzyme working rates. For example‚ most living things
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ASSAYING PLANT SUBSTANCES FOR ANTIBACTERIAL ACTIVITY PURPOSE To test plant extracts to see if they possess antibacterial activity. PROCEDURE First create a list of plant extracts being tested and choose 2 extracts per group. The plants being tested are Oregano‚ Tea tree‚ Eucalyptus and Colloidal Silver. Obtain two nutrient agar plates and using a permanent marker draw four quadrants on the bottom of each agar plate. Using a sterile pipet transfer 250 ml of E. coli broth to the middle of
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