For this experiment‚ we started off by taking tubes numbered 1-4 and started adding one scoop of our enzyme catalyst‚ in this case‚ the yeast. We then proceeded to measure and add 1 mL of distilled water to test tubes A-D. To get a more accurate measure of 1 mL of distilled water‚ we used the dropper labeled “W” to drop distilled water into the 5 mL graduated cylinder until we saw that the bottom of the water line reached closely to 1 mL. Next‚ we took the four tubes with the scoop of yeast and
Premium Chemistry Water Distillation
Enzyme assay lab report Health and safety: 4-nitrophenol is harmful. Introduction: Enzymes are quaternary structured proteins that are specific biological catalysts that speed up a reaction without being used up. They contain an active site that allows substrate to bind to a specific area on the enzyme which is of a complimentary shape of the substrate. There are two models of enzyme action‚ the Lock and Key model and the Induced Fit model. The Lock and Key model states that the enzyme has a specific
Premium Enzyme
Enzyme Lab 6 03/13/2013 Report by Mary Jo Anthony I. Introduction II. Materials and Methods III. Results IV. Conclusion and Discussion Introduction Background Information: This lab allowed us to study chemical reactions and how catalysts will affect the rate of these reactions. The reaction we studied is the breakdown of hydrogen peroxide to water and oxygen and it is vital to life. The molecule hydrogen peroxide is a molecule that is toxic
Premium PH Enzyme Chemical reaction
Abstract Enzyme catalysis was observed in order to analyze how changes in temperature‚ pH‚ enzyme concentration‚ and substrate concentration affected an enzyme-catalyzed reaction. This experiment analyzed the rate of enzyme-catalyzed reactions and observed the correlation between catalase activity and products formed. It was found out that the rate of an enzyme-catalyzed reaction starts off rapidly‚ decreases‚ and levels off or completely stops‚ and can be further affected by environmental factors
Premium Enzyme Catalysis Chemical reaction
Catalase Enzyme Lab Samiya Hussein March 9‚ 2012 Introduction In order to receive the necessary amounts of energy required for daily function‚ the digestive system must break down proteins‚ fats and carbohydrates. In doing so‚ the body produces poisonous chemicals; however‚ the cells aren’t harmed. This is because enzymes are used to break down these chemicals. The name of the enzyme that was the main focus of the lab is catalase. Catalase is responsible for catalyzing hydrogen peroxide
Premium PH Enzyme Hydrogen peroxide
Purpose: The purpose of this lab was to observe and understand the effects of changes in temperature‚ pH‚ enzyme concentration‚ and substrate concentration on the reaction rate of an enzyme-catalyzed reaction. Another purpose of the lab was to explain how environmental factors affect the rate of enzyme-catalyzed reactions. Hypothesis: I believe that if there is an increase in enzyme concentration‚ an increase in temperature‚ or an increase in pH‚ then the intensity of the reaction will
Premium Chemistry Chemical reaction Enzyme
NAME: Aleema Chelsea Chinchamee LAB PARTNERS: Karishma Ramrattan‚ Vishma Ramsumair and Sharona Badree ID #: 814003081 DATE (of lab session): Tuesday 24th March‚ 2015 DEMONSTRATOR: Maurissa Course Code & Title: BIOL 1362- Biochemistry I Title of Lab: Investigating Enzymatic Activity in Sweet Potato‚ Irish Potato Extract and Milk. Aims: 1. To determine the effect of ascorbic acid on Polyphenol Oxidase (Phenolase). 2. To determine the level of specificity of Phenolase using the
Premium Enzyme
Sang Kim Enzyme Catalyst Purpose/Problem: There are four parts to the Enzyme Catalyst lab - Activity A‚ B‚ C‚ and D. In activity A‚ the characteristics of enzyme actions will be observed. The main purposes are to determine the rate of an enzyme catalyzed reaction‚ to study the characteristics of an enzyme mediated reaction‚ and to observe the effect of heat on enzyme activity. The purpose of activity B is to use the Titration Protocol to determine the initial amount of H 2O2 present
Premium Enzyme Catalysis Catalase
Enzyme Catalysis Lab Problem: Before the lab‚ one should understand: • The general functions and activities of enzymes; • The relationship between the structure and function of enzymes • The concept of initial reaction rates of enzymes; • How the concept of free energy relates to enzyme activity; • That change in temperature‚ pH‚ enzyme concentration‚ and substrate concentration can affect the initial reaction rates of enzyme-catalyzed reactions; and • Catalyst‚ catalysis‚ and catalase
Premium Enzyme Chemical reaction Catalysis
pH on Enzyme Activity Athenaa Jeyachandran Mr.Menes Course Code January 21st‚ 2013 The Effect on Enzyme Activity Due to Temperatures Purpose: The purpose of this lab experiment is to examine how different temperatures affect enzyme activity using hydrogen peroxide. Hypothesis: My hypothesis for this experiment is that temperatures near body temperature is when enzyme activity will be at its highest. I believe this will occur because in our body‚ enzymes are very
Premium Temperature Celsius Fahrenheit