ACC is then converted to ethylene by ACC oxidase (ACO)‚ a member of the oxygenase/oxidase superfamily of enzymes6.The ACS plays a central role to regulate ethylene production through changes in ACS gene expression levels and the stability/activity of the enzyme. ACS proteins can be divided into 3 groups (type-1‚ 2 & 3) based on their C-terminal domain and the presence of phosphorylation sites. Type-1 and -2 ACS isoforms contain phosphorylation sites for kinases‚ but type-3 lacks of the phosphorylation
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concentration increases‚ the rate of reaction will increase respectively‚ until the enzyme reaches its optimum point of saturation‚ after which any increase in the substrate concentration will no longer affect the rate of reaction. The independent variable in this investigation is the varying concentrations of the substrate (Hydrogen Peroxide: 1%‚ 3%‚ 5% and 6%)The dependent variable was the rate of enzyme catalase activity‚ which was measured by the volume of froth produced after one minute.The concentration
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Microscope lab report Introduction Microscope is a tool used to enlarge images of small objects that are hard to study with bare eyes. The compound light microscope‚ which is going to be used in this lab activity‚ is an instrument with two lenses and various knobs to focus the image. In this lab‚ we will learn about the proper use and handling of the microscope. Objectives: •Demonstrate the appropriate procedures used while using the compound light microscope correctly. •Make and use a wet mount
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Discussion Our results indicate that the presence of iron increases the rate of the peroxidase’s activity‚ judging from the increase in absorbance with increasing amount of iron. This result supports our hypothesis that the iron acts as a cofactor in peroxidase’s activity and results in the faster rate of the reaction. Also‚ we assumed that the more we put iron in the solutions‚ the faster the rate is. This assumption is also supported by the results that higher concentration of iron cofactors shows
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6 November 2012 SUBJECT: Lab Report Analysis This memo proposes the observations and claims I collected from reviewing three different lab reports. Three fields of study are composed within this memo that includes Electrical Engineering‚ Environmental Engineering‚ and Petroleum Engineering. “Electrical Filters‚” (Electrical)‚ written by Joe Schmoe‚ is a lab report made by a student at a university. The College Board produced an environmental lab report named‚ “Monitoring Air Quality‚”
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when the enzyme and buffer in the assay are held constant were experimented. We analyzed the change in absorbencies over time for varying substrate concentrations. There were four experimental assays which contained 1% enzyme solution‚ substrate solution of 0%‚ 1%‚ 2%‚ and 3% concentrations‚ guaiacol‚ and pH 7 buffer. At 2% concentration there was a greater enzymatic activity and at 3% concentration enzymatic activity decreased. The results were supported of our hypothesis. INTRODUCTION Enzymes are proteins
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the mouth‚ where an enzyme‚ salivary amylase (α-amylase;ptyalin) starts to breaking the polysaccharides (starch) into short polysaccharides (dextrin). Dextrin is a partial degradation of starch‚ shorter chains of maltose units. Salivary amylase is inactivated by stomach acid in the stomach and to a small extent‚ it continues breaking down starch (but there’s no enzymatic activities on carbohydrates in the stomach). An intestinal enzyme‚ pancreatic amylase‚ continues the activity
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the acyl-enzyme is then hydrolysed by nucleophilic attack of serine on the peptide carbonyl group‚ collapse of the tetrahedral intermediate and the release of the amine component. Histidine 57 then acts as a general acid catalyst and draws a proton away from the water molecule. The OH- ion then attacks the carbonyl carbon atom of the acyl group which forms another tetrahedral intermediate which breaks down to form the carboxylic acid product. The release of this acid then frees up the enzyme to continue
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diseases (Labbe et al.‚ 2008). Also‚ many drugs produced contain drug classes that can inhibit the function of mitochondria by preventing or altering a stage in ATP production in the Krebs cycle or electron transport chain or acting as an inhibitor of an enzyme that carries out a certain reaction in citric acid cycle or ETC (Dykens and Will‚ 2007). These drugs are therefore pretested
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INTRODUCTION As for the visual documentation of a living or dead specimen‚ digital photography has largely replaced traditional illustrations of the living specimen as the standard method of recording the colour and anatomy of the specimen in the field today. Earlier traditional illustration (coloured and black and white) are still considered scientifically important because they can stress fine anatomical features that are often obscured by liquid. Even today‚ these earlier traditional illustrations
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