Enzyme Lab Name ___________________________ Assignment 1: Getting to Know Enzyme Lab: Setting Up an Experiment The first screen that appears in Enzyme Lab shows you a biochemistry lab containing all the reagents and equipment you will need to perform your experiments. Click on each item in the lab to learn more about its purpose. Once you are familiar with the lab‚ click on the Experiment button to begin the first assignment. This assignment is designed to help you become
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Matthew Saldanha Bio DCP lab-Catalase experiment Aim: To investigate enzyme kinetics‚ using different concentration of the enzyme. Hypothesis: The assay system used in the lab consists of a filter paper disc coated with the enzyme and the dropped into a papercup of substrate (Hydrogen Peroxide). As the hydrogen breaks down the hydrogen peroxide into hydrogen and oxygen gas‚ the bubbles of oxygen gas collect underneath the filter and make it
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Interpretation and Evaluation of Results Interpretation: The results from the experiment determining the effects of pH on enzyme activity show that as the independent variable‚ pH‚ increases the dependent variable‚ percentage transmission‚ decreases. This is shown in the results as at the lowest pH‚ pH 4.0 the average percentage transmission is at its highest at 97%. At the highest pH‚ pH 8.0 the average transmission is 78.5%. This is also supported from the graph as it produces a negative gradient
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containing these four unknown solutions as flask A‚ flask B‚ flask C‚ and flask D. Design an experiment‚ based on the principles of diffusion and osmosis‚ that the assistant could use to determine which of the flasks contains each of the four unknown solutions. Include in your answer: a. a description of how you would set up and perform the experiment; b. the results you would expect from your experiment; and c. an explanation of those results based on the principles involved. Be sure to clearly
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Aim To investigate the effect of different temperatures on the activity of rennin in milk Introduction Enzymes are globular protein‚ responsible for most of the chemical activities of living organisms. They are made up of long chains of amino acids containing carbon‚ hydrogen‚ oxygen and nitrogen (Gunsch‚ 2012). The role of enzyme is to act as catalysts‚ substances that speed up chemical reactions without being chemically altered during the process. The speeding up of chemical reactions is done
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being carried out is to observe the effects of temperature on starch break down using a synthesized version of salivary amylase‚ this being Diastase. The starch will be placed into the Diastase and water and then placed in baths of water of different l. temperatures. The test tube containing water will have little or no reaction at all. However‚ the test tube containing the Diastase will have several different times to which the starch will break down. Once the Diastase reaches between 30
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Experiment 4: Enzyme Kinetics. Results/Discussion Week 1 Part A: Table 1. Enzyme activity for each assay of 4-nitroaniline formation. Rate of 4-nitroaniline formation Name of trial Abs/sec Abs/min M/min mol/min µmol/min #1 0.00003 0.0018 2.05x10-7 2.15 x10-10 2.15 x10-4 # 2 0.00010 0.0060 6.81x10-7 7.15x10-10 7.15x10-4 # 3 0.00020 0.0120 1.36x10-6 1.43x10-9 1.43x10-3 # 4 0.00030 0.0180 2.00x10-6 2.10x10-9 2.10x10-3
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Concentration On The Activity Of The Enzyme Catalase A Level Biology Project Aims This is an experiment to examine how the concentration of the substrate hydrogen peroxide affects the rate of reaction of the enzyme catalase. Background Information Enzymes such as Catalase are protein molecules which are found in living cells. They are used to speed up specific reactions in the cells. They are all very specific as each enzyme just performs one particular reaction. Catalase is an enzyme found in food
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Investigating the effect of pH on amylase activity Aim The aim of the experiment is to determine the effects of different pH and the rate of reaction on fungal amylase and starch. Introduction The enzyme amylase is found in the human body‚ it catalyses the hydrolosis of internal glycosidic bonds in polysaccharides‚ the breakdown of starch into sugars. Amylase is present in human saliva‚ where it initiates the chemical process of digestion. Enzymes work best at an optimum pH of 7 which is the bodies
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Lab Report (Effect of concentration on enzyme activity) Biology Noor Alawadhi 11- KC Introduction: An Enzyme is a protein‚ which is capable of starting a chemical reaction‚ which involves the formation or breakage of chemical bonds. A substrate is the surface or material on or from which an organism lives‚ grows‚ or obtains its nourishment. In this case it is hydrogen peroxide. This lab report will be explaining the experiment held to understand the effects of the changes in the amount of
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