Affect of enzyme concentration to the rate of reaction Aim: With the experiment of protein solution‚ in this case egg white added to different pepsin concentrations (0%‚ 0.2%‚ 0.4%‚ 0.6%‚ 0.8%‚ 1.0%) shows‚ as the egg white is a protein and the pepsin works as an enzyme‚ how a higher pepsin concentration and therefore a larger amount of enzymes effect the rate of reaction. Hypothesis: An increased concentration of pepsin speeds up the time the mixture needs to come clear. Introduction:
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of this lab was to measure the extent of enzyme reaction on given substrates by means of color change. The reaction followed is given below: Tyrosinase³ Enzyme Pyrocatechol Hydroxyquinone Oxidation/Reduction Pink ³ Brown E+S + [ES] = E+P Enzyme Reaction Hypothesis: If there is an increase in enzyme concentration‚ an increase in reaction temperature‚ or an increase in buffer pH‚ then greater intensity in a given reaction
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Experiment 10 – Enzymes Enzymes are proteins that act as catalysts for biological reactions. Enzymes‚ like all catalysts‚ speed up reactions without being used up themselves. They do this by lowering the activation energy of a reaction. All biochemical reactions are catalyzed by enzymes. Since enzymes are proteins‚ they can be denatured in a variety of ways‚ so they are most active under mild conditions. Most enzymes have optimum activity at a neutral pH and at body temperature. Enzymes are
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process of distillation. Distillation is the separation of volatile substances mixed with nonvolatile substances by boiling the mixture to alter the phase of the volatile substance. The researchers used the simple distillation set up to conduct the experiment. 25 ml of The Bar Vodka was used and 7 ml of distillate was collected before a temperature of 95 degrees Celsius was reached. Collected distillate was then subjected to a flammability test to confirm ethanol content. The percent ethanol was computed
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Purpose: The purpose of this lab was to determine the freezing point of pure water‚ the experimental van’t Hoff factor for a substance and the molar mass of an unknown solution. Reagents: 25mL deionized Water ¼ to ½ inches of rock salt 25mL Sodium Nitrate (NaNO3 ) 25mL unknown solution – handle unknown solution carefully Procedure: For part 1‚ the freezing point of deionized water was determined. An ice bath was prepared by filling a 600mL beaker ¾ full of ice and covered with ¼ to
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relationship between substrate concentration and initial reaction rate provided that substrate concentration is much greater than enzyme concentration. Enzymes are essential to life as they are required for many vital metabolic reactions to occur. To adequately explain the properties of enzymes‚ it is assumed that an enzyme-controlled reaction takes place through an enzyme-substrate complex by the lock and key mechanism. It is hypothesized that a greater concentration of product is achieved through
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AIR POLLUTION IN THE PHILIPPINES Guides and Tips Home / Blog / Air Pollution in the Philippines Air pollution is a common and frequent problem nowadays. When you are on your way to work‚ you pass by your neighbors’ backyard where he is currently burning their garbage from yesterday‚ then you come across a smoke-belching public utility vehicle‚ then a guy beside you lighted a cigarette and started suffocating you with second-hand smoke. You cannot always depend on your handkerchief on covering
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Abstract: The Enzyme Lab results where when the liver was frozen‚ its reaction was fast‚ and when it was hot‚ it was slow‚ and the liver that was at room temperature reacted slowly to medium. Introduction: The Enzyme Lab is to conduct investigations to determine the most favorable conditions for the most efficient enzyme activity. Variables to be used testing include temperature‚ pH values and surface area. Enzymes are proteins that speed up the rate of chemical reactions‚ which would otherwise
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Enzymes are organic catalysts; where a substance speeds up the rate of chemical reactions without changing being changed by the reactions. In lab they tested this by seeing how H2O2 and the catalysts from the banana and liver react to make H2O+O2. Depending on different conditions; like decomposition of H2O2 (surface area)‚ temperature on function‚ reusing the catalase‚ reaction rate of iced liver returned to room temperature‚ and effects of pH on enzyme activity‚ to see how much O2 was released
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Name: _____________________ Date: _____________ Student Exploration: pH Analysis Vocabulary: acid‚ acidic‚ alkaline‚ base‚ indicator‚ neutral‚ pH Prior Knowledge Questions (Do these BEFORE using the Gizmo.) 1. Acids are substances that produce hydrogen ions (H+) when dissolved in water. Lemon juice is an example of an acid. A. What does lemon juice taste like? __Sour.______________________________ B. What does it feel like if lemon juice gets in your eye? ___It
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