Received July 18‚ 1997X A radiochemical enzyme assay for studying cyclooxygenase (COX)-catalyzed prostaglandin biosynthesis in vitro was optimized with respect to both COX-1 and COX-2 activity. The assay can be used to assess the relative selectivity of plant-derived inhibitors on COX-1 and COX-2. Assay conditions were optimized for both enzymes with respect to concentration of cofactors (l-epinephrine‚ reduced glutathione‚ and hematin)‚ activation time (enzyme and cofactors)‚ reaction time‚ and
Premium Prostaglandin
| Peroxisome in animal cell | Life sciences‚ HAN university of applied science‚ Nijmegen‚ Netherlands | | Mohsen Ghanbari | 30-12-2012 | Peroxisome in animal cell Author: Mohsen Ghanbari Abstract Peroxisomes have an essential function in cell metabolism. [1] They are small‚ membrane bounded organelles that have at least fifty different enzymes for several metabolic reactions. [2] Their appearance is like lysosomes; however‚ their main function is oxidation reactions especially oxidation
Premium Cell Metabolism Eukaryote
structure of these cells is a flying disk shaped like a donut‚ this is so maximum haemoglobin can be carried allowing maximum amount of oxygen to be transported. Sperm cells A sperm cell is the male reproductive cell that carries the male portion of chromosomes and deoxyribonucleic acid (DNA) information to be fused with the female egg or ovum. The sperm cell carries various amount of heredity information inside the cell nucleus. There are various ways in which sperm can be transmitted to the
Free Cell Eukaryote Cell nucleus
The act of wagering on the outcome of an event is one of mankind’s oldest activities. Gambling has been present in ancient civilizations and gained its prevalence during the Ancient Olympic Games. But when it comes to the oldest form of gambling‚ Sports betting‚ there are just a handful of locations where it is legal to wager on the outcome of amateur or professional sports. The fact that wagering on sports is illegal in the majority of the United States is puzzling when it plays such a large role
Premium
pH‚ and concentration of substrate‚ on the activity of the enzymes. By conducting these three separate experiments also‚ three graphs are able to be obtained where the trend of each factor affecting on the enzyme activity is shown and described clearly. II. Hypothesis Experiment 1 (Effect of Temperature): As the temperature increases‚ the height of the bubble will increase too‚ indicating a faster rate of reaction. Experiment 2 (Effect of pH): Enzymes are affected by changes in pH. Extremely
Premium Enzyme Chemical reaction Chemistry
Introduction Enzymes are key players in metabolism. A metabolism is the organic processes in a cell or an organism that are necessary for life. An enzyme affects the rate at which a reaction occurs when the activation energy is lowered. In this reaction the reactant is called the substrate which is that combine with enzymes molecules to form a temporary enzyme substrate complex. During this products are formed and the enzyme molecules released is unchanged. For the substrate complex to form the
Premium Enzyme Metabolism Chemical reaction
Cells of the body are organized into four fundamental tissues; epithelial‚ connective‚ muscle and nerve. Epithelial tissues line all surfaces of the body: the skin‚ cavities‚ ducts‚ vessels. They are named according to the cell shape on the free surface. Cells of a tissue are held together by the basement membrane and intercellular fibbers. These tissues receive their nutrition by diffusion‚ for they are without blood vessels. Simple squamous epithelium lines all blood and lymphatic vessel‚ including
Premium Epithelium Ureter Urinary bladder
study of the concern performance . * Major project :project on ’SRI GARMENTS IN YARN MANAGEMENT SYSTEM’‚V L B janakiammal college of arts and science * POST GRADUATION * Mini project: Summer internship program on HRM and PRODUCTION activities at SRI DHARRN INDUSTRIES‚ Coimbatore‚ June 2011. In this summer internship program the project done on two departments HRM and
Premium Human resource management Cricket Coimbatore
STUDY GUIDE WITH CELL QUIZ Cell wall: Cell membrane: All cells have cell membrane Allows only essential things to come in. Double layer of lipids. Double layer=bilayer Golgi body Process and package macromolecules There is a cis end and a Trans end. Cells secrete things that have been packages from apparatus. Modify by adding carbs and phosphates. Imports substances Endoplasmic retulum Ribosome Cytoplasmic granules-small particle that floats in cytoplasm Made of ribosomal DNA and
Premium Cell Protein Organelle
CHAPTER 12- THE CELL CYCLE I. Concept 12.1- Cell division results in genetically identical daughter cells A. Overview 1) The continuity of life is based on the reproduction of cells or cell division. 2) The cell division process is an integral part of the cell cycle‚ the life of a cell from the time it is first formed from a dividing parent cell until its own division into two cells. B. Cellular Organization of the Genetic Material 1) A cell’s endowment of DNA‚ its genetic
Premium Cell cycle Mitosis Chromosome